Appetite Regulation, Growth Performances and Fish Quality Are Modulated by Alternative Dietary Protein Ingredients in Gilthead Sea Bream (Sparus aurata) Culture

SIMPLE SUMMARY: In a world dramatically harassed by climate changes and overexploitation of resources, the aquaculture expansion poses several challenges. Among the others, aquafeed formulations need to be rethought in a circular economy vision, avoiding food-feed competition, and possibly valorizing one or more ingredients for their functionality. In this context, the present trial showed that Hermetia illucens prepupae, poultry-by products, and red swamp crayfish meals can effectively replace a substantial proportion of vegetable proteins in the diet for gilthead seabream (Sparus aurata) without impairing fish growth and fillet quality. On the contrary, a blend of Tisochrysis lutea and Tetraselmis suecica negatively impacted fish growth and further studies are thus necessary to better valorize their natural content in pigments in fish farming. ABSTRACT: By answering the need for increasing sustainability in aquaculture, the present study aimed to compare growth, gene expression involved in appetite regulation, physical characteristics, and chemical composition of Sparus aurata fed alternative protein sources. Fish were fed ten iso-proteic, iso-lipidic, and isoenergetic diets: a vegetable-based (CV) and a marine ingredient-rich (CF) diet were set as control diets. The others were prepared by replacing graded levels (10, 20 or 40%) of the vegetable proteins in the CV with proteins from a commercial defatted Hermetia illucens pupae meal (H), poultry by-product meal (PBM) singly (H10, H20, H40, P20, P40) or in combination (H10P30), red swamp crayfish meal (RC10) and from a blend (2:1, w:w) of Tisochrysis lutea and Tetraselmis suecica (MA10) dried biomasses. The increase in ghre gene expression observed in MA10 fed fish matched with increased feed intake and increased feed conversion ratio. Besides, the MA10 diet conferred a lighter aspect to the fish skin (p < 0.05) than the others. Overall, no detrimental effects of H, PBM, and RC meal included in the diets were observed, and fish fatty acid profile resulted as comparable among these groups and CV, thus demonstrating the possibility to introduce H, PBM, and RC in partial replacement of vegetable proteins in the diet for Sparus aurata.