Comparison of the Effect of High-Intensity Laser Therapy (HILT) on Skin Surface Temperature and Vein Diameter in Pigmented and Non-Pigmented Skin in Healthy Racehorses

SIMPLE SUMMARY: High-intensity laser therapy (HILT) is used in the treatment of horses, but little is known about the differences in the impact of HILT performed on pigmented and non-pigmented skin. The aim of this study was to assess differences in the influence of HILT on skin surface temperature and vein diameter in a group of healthy racehorses with pigmented and non-pigmented skin in the treatment area. The hypothesis was that HILT would cause a greater increase in skin surface temperature and vein diameter in horses with pigmented skin compared to non-pigmented skin. Ten Thoroughbreds with pigmented skin and ten Thoroughbreds with non-pigmented skin in the treatment area received HILT. Changes in the vein diameter and skin surface temperature of the irradiated area were measured before and after HILT. The HILT treatment caused an increase in the pigmented skin surface temperature and a decrease in the non-pigmented skin surface temperature, while the vein diameter increased in both groups. In conclusion, melanin content in the epidermis plays an important role in light energy absorption and photothermal effects. Determining the physiological and clinical effects of HILT performed on pigmented and non-pigmented skin will help clinicians choose appropriate HILT parameters. ABSTRACT: The aim of the study was to assess differences in the influence of high-intensity laser therapy (HILT) on the skin surface temperature and vein diameter of the lateral fetlock joint region in a group of racehorses with pigmented and non-pigmented skin in the treatment area. Twenty Thoroughbreds were divided into two equal groups: pigmented and non-pigmented skin groups. Each horse received the same HILT treatment. Just before and immediately after HILT, thermographic examination was performed to measure the skin surface temperature and ultrasonographic examination assessed the lateral digital palmar vein diameter. After HILT, the pigmented skin surface temperature increased, while the non-pigmented skin surface temperature decreased, and the difference between both groups was significant (p < 0.001). The vein diameter increased after HILT in horses with pigmented and non-pigmented skin, but the difference between both groups was not significant (p = 0.14). In conclusion, melanin content in the epidermis plays an important role in light energy absorption and photothermal effects. The vein diameter changes after HILT application indicated that the increase in vessel diameter may partly depend on photothermal mechanisms occurring in irradiated tissue. Further research is necessary to describe the physiological and clinical effects of HILT performed on pigmented and non-pigmented skin.