Use of Hydrolyzed Chinese Gallnut Tannic Acid in Weaned Piglets as an Alternative to Zinc Oxide: Overview on the Gut Microbiota

SIMPLE SUMMARY: The effects of dietary hydrolyzed Chinese gallnut tannic acid (GCT) as a replacement for ZnO were investigated on weaned piglets. A total of 72 piglets (31 ± 1 day) were selected and divided randomly into two groups: a control group, with a basal diet of + 1600 mg/kg ZnO; and a treated group, with a basal diet of + 1899.5 mg/kg GCT. The diarrhea rate of piglets in the treated group declined on days 14–21 than in the control group. Additionally, we found GCT can reduce the crypt depth of the ileum and improve antioxidant capacity. High throughput sequencing showed that the GCT increased the richness of bacteria (Lachnospiraceae, Prevotella, and Lactobacillus amylovorus) associated with the degradation of cellulose and hemicellulose. These data indicate that 1899.5 mg/kg GCT could be an alternative for 1600 mg/kg ZnO in the diet of piglets. ABSTRACT: The effects of dietary hydrolyzed Chinese gallnut tannic acid(GCT) as a replacement for ZnO were investigated on weaned piglets. A total of 72 weaned piglets at 31 ± 1 day (six replicate pens per treatment with six piglets per pen) were selected and divided randomly into two groups: a control group, with a basal diet of + 1600 mg/kg ZnO; and a treated group, with a basal diet of + 1899.5 mg/kg GCT. Data analysis showed that the significance of average daily gain and average daily feed intake between the two groups was p = 0.731 and p = 0.799, respectively. Compared with the control group, the diarrhea rate of piglets in the treated group underwent no noticeable change on days 0–7 (p = 0.383) and 7–14 (p = 0.263), but decreased significantly on days 14–21 (p < 0.05). Additionally, we found GCT can reduce the crypt depth of the ileum and improve its antioxidant capacity (p < 0.05). High throughput sequencing showed that GCT increased the richness of the bacteria Lachnospiraceae (p = 0.005), Prevotella_2 (p = 0.046) and Lactobacillus amylovorus (p = 0.081), which are associated with the degradation of cellulose and hemicellulose. The study indicated that 1899.5 mg/kg GCT could be an alternative for 1600 mg/kg ZnO in the diet of piglets.