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Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome

Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with t...

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Autores principales: Marrazzo, Pasquale, Pizzuti, Valeria, Zia, Silvia, Sargenti, Azzurra, Gazzola, Daniele, Roda, Barbara, Bonsi, Laura, Alviano, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300685/
https://www.ncbi.nlm.nih.gov/pubmed/34206190
http://dx.doi.org/10.3390/antibiotics10070750
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author Marrazzo, Pasquale
Pizzuti, Valeria
Zia, Silvia
Sargenti, Azzurra
Gazzola, Daniele
Roda, Barbara
Bonsi, Laura
Alviano, Francesco
author_facet Marrazzo, Pasquale
Pizzuti, Valeria
Zia, Silvia
Sargenti, Azzurra
Gazzola, Daniele
Roda, Barbara
Bonsi, Laura
Alviano, Francesco
author_sort Marrazzo, Pasquale
collection PubMed
description Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms.
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spelling pubmed-83006852021-07-24 Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome Marrazzo, Pasquale Pizzuti, Valeria Zia, Silvia Sargenti, Azzurra Gazzola, Daniele Roda, Barbara Bonsi, Laura Alviano, Francesco Antibiotics (Basel) Perspective Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms. MDPI 2021-06-22 /pmc/articles/PMC8300685/ /pubmed/34206190 http://dx.doi.org/10.3390/antibiotics10070750 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Perspective
Marrazzo, Pasquale
Pizzuti, Valeria
Zia, Silvia
Sargenti, Azzurra
Gazzola, Daniele
Roda, Barbara
Bonsi, Laura
Alviano, Francesco
Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome
title Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome
title_full Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome
title_fullStr Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome
title_full_unstemmed Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome
title_short Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome
title_sort microfluidic tools for enhanced characterization of therapeutic stem cells and prediction of their potential antimicrobial secretome
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300685/
https://www.ncbi.nlm.nih.gov/pubmed/34206190
http://dx.doi.org/10.3390/antibiotics10070750
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