Sex Hormones in Hemolymph of Red King Crabs from the Barents Sea

SIMPLE SUMMARY: Well-known sex hormones, testosterone and 17β-estradiol, play a crucial role in the reproduction of vertebrates. Biochemical assays have detected these substances in a few crustaceans, and it has been hypothesized that these hormones are involved in the regulation of crustacean reproduction. Red king crab is a large commercially important species harvested both in their native areas (North Pacific) and in the area of its introduction (Barents Sea). The presence of 17β-estradiol and testosterone and fluctuations of their concentrations in relation to different factors have not yet been investigated. For this reason, we provided a pilot study to reveal the levels of sex hormones in hemolymph of red king crabs captured in the coastal Barents Sea. These hormones were detected in the crabs and we compared our data with previously published data involving a wide range of crustaceans. We found seasonal variations in the level of testosterone with the maximum in the spawning period. Our data expand the current knowledge about the red king crab physiology and may be used for the development of its aquaculture. ABSTRACT: The presence of vertebrate-related steroid sex hormones has been reported in both freshwater and marine crustaceans. However, despite the commercial importance of king crabs, many aspects of their endocrinology are still unknown. For this reason, we examined hemolymph samples of the red king crab Paralithodes camtschaticus from the Barents Sea population for the presence of testosterone and 17β-estradiol using radioimmunoassay. The mean testosterone concentration was 0.46 ± 0.04 (range 0.08–1.39) ng mL(–1), whereas the mean 17β-estradiol concentration was 1248.9 ± 91.4 (range 217.7–4100.1) pg mL(–1). In general, the levels of 17β-estradiol and testosterone in red king crabs were higher than reported for the hemolymph of amphipods, crabs, and shrimps from warm and temperate waters, probably because the king crabs analyzed were larger and heavier than the other crustaceans. The concentrations of sex steroids did not differ significantly between males and females and between immature and mature red king crabs. Seasonal variations in the level of testosterone with the maximum value in the spawning period (May) indicate a potential role of the sex hormones in the maturation and reproduction processes of red king crab. Taking into account the slow growth rate in P. camtschaticus, our data could be useful not only for further physiological studies but also for the development of reliable techniques for red king crab aquaculture.