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Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra

An application of solid (13)C nuclear magnetic resonance (NMR) spectroscopy for the determination of macronutrients, total polyphenols content, antioxidant activity, N C S elements, and pH in commercially available bee pollens is reported herein. Solid-state (13)C NMR spectra were recorded for homog...

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Autores principales: Mazurek, Sylwester, Szostak, Roman, Kondratowicz, Mateusz, Węglińska, Magdalena, Kita, Agnieszka, Nemś, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301116/
https://www.ncbi.nlm.nih.gov/pubmed/34356356
http://dx.doi.org/10.3390/antiox10071123
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author Mazurek, Sylwester
Szostak, Roman
Kondratowicz, Mateusz
Węglińska, Magdalena
Kita, Agnieszka
Nemś, Agnieszka
author_facet Mazurek, Sylwester
Szostak, Roman
Kondratowicz, Mateusz
Węglińska, Magdalena
Kita, Agnieszka
Nemś, Agnieszka
author_sort Mazurek, Sylwester
collection PubMed
description An application of solid (13)C nuclear magnetic resonance (NMR) spectroscopy for the determination of macronutrients, total polyphenols content, antioxidant activity, N C S elements, and pH in commercially available bee pollens is reported herein. Solid-state (13)C NMR spectra were recorded for homogenized pollen granules without chemical treatment or dissolution of samples. By combining spectral data with the results of reference analyses, partial least squares models were constructed and validated separately for each of the studied parameters. To characterize and compare the models’ quality, the relative standard errors of prediction (RSEP) were calculated for calibration and validation sets. In the case of the analysis of protein, fat and reducing sugars, these errors were in the 1.8–2.5% range. Modeling the elemental composition of bee pollen on the basis of (13)C NMR spectra resulted in RSEP(cal)/RSEP(val) values of 0.3/0.6% for the sum of NHCS elements, 0.3/0.4% for C, 1.8/1.9% for N, and 4.2/6.1% for S quantification. Analyses of total phenolics and ABTS antioxidant activity resulted in RSEP values in the 2.7–3.5% and 2.8–3.8% ranges, respectively, whereas they were 1.4–2.1% for pH. The obtained results demonstrate the usefulness of (13)C solid-state NMR spectroscopy for direct determination of various important physiochemical parameters of bee pollen.
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spelling pubmed-83011162021-07-24 Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra Mazurek, Sylwester Szostak, Roman Kondratowicz, Mateusz Węglińska, Magdalena Kita, Agnieszka Nemś, Agnieszka Antioxidants (Basel) Article An application of solid (13)C nuclear magnetic resonance (NMR) spectroscopy for the determination of macronutrients, total polyphenols content, antioxidant activity, N C S elements, and pH in commercially available bee pollens is reported herein. Solid-state (13)C NMR spectra were recorded for homogenized pollen granules without chemical treatment or dissolution of samples. By combining spectral data with the results of reference analyses, partial least squares models were constructed and validated separately for each of the studied parameters. To characterize and compare the models’ quality, the relative standard errors of prediction (RSEP) were calculated for calibration and validation sets. In the case of the analysis of protein, fat and reducing sugars, these errors were in the 1.8–2.5% range. Modeling the elemental composition of bee pollen on the basis of (13)C NMR spectra resulted in RSEP(cal)/RSEP(val) values of 0.3/0.6% for the sum of NHCS elements, 0.3/0.4% for C, 1.8/1.9% for N, and 4.2/6.1% for S quantification. Analyses of total phenolics and ABTS antioxidant activity resulted in RSEP values in the 2.7–3.5% and 2.8–3.8% ranges, respectively, whereas they were 1.4–2.1% for pH. The obtained results demonstrate the usefulness of (13)C solid-state NMR spectroscopy for direct determination of various important physiochemical parameters of bee pollen. MDPI 2021-07-14 /pmc/articles/PMC8301116/ /pubmed/34356356 http://dx.doi.org/10.3390/antiox10071123 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mazurek, Sylwester
Szostak, Roman
Kondratowicz, Mateusz
Węglińska, Magdalena
Kita, Agnieszka
Nemś, Agnieszka
Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra
title Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra
title_full Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra
title_fullStr Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra
title_full_unstemmed Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra
title_short Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra
title_sort modeling of antioxidant activity, polyphenols and macronutrients content of bee pollen applying solid-state (13)c nmr spectra
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301116/
https://www.ncbi.nlm.nih.gov/pubmed/34356356
http://dx.doi.org/10.3390/antiox10071123
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