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Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra
An application of solid (13)C nuclear magnetic resonance (NMR) spectroscopy for the determination of macronutrients, total polyphenols content, antioxidant activity, N C S elements, and pH in commercially available bee pollens is reported herein. Solid-state (13)C NMR spectra were recorded for homog...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301116/ https://www.ncbi.nlm.nih.gov/pubmed/34356356 http://dx.doi.org/10.3390/antiox10071123 |
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author | Mazurek, Sylwester Szostak, Roman Kondratowicz, Mateusz Węglińska, Magdalena Kita, Agnieszka Nemś, Agnieszka |
author_facet | Mazurek, Sylwester Szostak, Roman Kondratowicz, Mateusz Węglińska, Magdalena Kita, Agnieszka Nemś, Agnieszka |
author_sort | Mazurek, Sylwester |
collection | PubMed |
description | An application of solid (13)C nuclear magnetic resonance (NMR) spectroscopy for the determination of macronutrients, total polyphenols content, antioxidant activity, N C S elements, and pH in commercially available bee pollens is reported herein. Solid-state (13)C NMR spectra were recorded for homogenized pollen granules without chemical treatment or dissolution of samples. By combining spectral data with the results of reference analyses, partial least squares models were constructed and validated separately for each of the studied parameters. To characterize and compare the models’ quality, the relative standard errors of prediction (RSEP) were calculated for calibration and validation sets. In the case of the analysis of protein, fat and reducing sugars, these errors were in the 1.8–2.5% range. Modeling the elemental composition of bee pollen on the basis of (13)C NMR spectra resulted in RSEP(cal)/RSEP(val) values of 0.3/0.6% for the sum of NHCS elements, 0.3/0.4% for C, 1.8/1.9% for N, and 4.2/6.1% for S quantification. Analyses of total phenolics and ABTS antioxidant activity resulted in RSEP values in the 2.7–3.5% and 2.8–3.8% ranges, respectively, whereas they were 1.4–2.1% for pH. The obtained results demonstrate the usefulness of (13)C solid-state NMR spectroscopy for direct determination of various important physiochemical parameters of bee pollen. |
format | Online Article Text |
id | pubmed-8301116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83011162021-07-24 Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra Mazurek, Sylwester Szostak, Roman Kondratowicz, Mateusz Węglińska, Magdalena Kita, Agnieszka Nemś, Agnieszka Antioxidants (Basel) Article An application of solid (13)C nuclear magnetic resonance (NMR) spectroscopy for the determination of macronutrients, total polyphenols content, antioxidant activity, N C S elements, and pH in commercially available bee pollens is reported herein. Solid-state (13)C NMR spectra were recorded for homogenized pollen granules without chemical treatment or dissolution of samples. By combining spectral data with the results of reference analyses, partial least squares models were constructed and validated separately for each of the studied parameters. To characterize and compare the models’ quality, the relative standard errors of prediction (RSEP) were calculated for calibration and validation sets. In the case of the analysis of protein, fat and reducing sugars, these errors were in the 1.8–2.5% range. Modeling the elemental composition of bee pollen on the basis of (13)C NMR spectra resulted in RSEP(cal)/RSEP(val) values of 0.3/0.6% for the sum of NHCS elements, 0.3/0.4% for C, 1.8/1.9% for N, and 4.2/6.1% for S quantification. Analyses of total phenolics and ABTS antioxidant activity resulted in RSEP values in the 2.7–3.5% and 2.8–3.8% ranges, respectively, whereas they were 1.4–2.1% for pH. The obtained results demonstrate the usefulness of (13)C solid-state NMR spectroscopy for direct determination of various important physiochemical parameters of bee pollen. MDPI 2021-07-14 /pmc/articles/PMC8301116/ /pubmed/34356356 http://dx.doi.org/10.3390/antiox10071123 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mazurek, Sylwester Szostak, Roman Kondratowicz, Mateusz Węglińska, Magdalena Kita, Agnieszka Nemś, Agnieszka Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra |
title | Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra |
title_full | Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra |
title_fullStr | Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra |
title_full_unstemmed | Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra |
title_short | Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State (13)C NMR Spectra |
title_sort | modeling of antioxidant activity, polyphenols and macronutrients content of bee pollen applying solid-state (13)c nmr spectra |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301116/ https://www.ncbi.nlm.nih.gov/pubmed/34356356 http://dx.doi.org/10.3390/antiox10071123 |
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