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Construction of a Three-Color Prism-Based TIRF Microscope to Study the Interactions and Dynamics of Macromolecules

SIMPLE SUMMARY: Prism-based single-molecule total internal reflection fluorescence (prismTIRF) microscopes are excellent tools for studying macromolecular dynamics and interactions. Here, we provide an easy-to-follow guide for the design, assembly, and operation of a three-color prismTIRF microscope...

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Detalles Bibliográficos
Autores principales:	Fairlamb, Max S., Whitaker, Amy M., Bain, Fletcher E., Spies, Maria, Freudenthal, Bret D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301196/ https://www.ncbi.nlm.nih.gov/pubmed/34201434 http://dx.doi.org/10.3390/biology10070571

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