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Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis

Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered b...

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Autores principales: Budvytyte, Rima, Milasiute, Akvile, Vitkus, Dalius, Strupas, Kestutis, Gulla, Aiste, Sakinyte, Ieva, Razumiene, Julija
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301313/
https://www.ncbi.nlm.nih.gov/pubmed/34210023
http://dx.doi.org/10.3390/biomedicines9070755
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author Budvytyte, Rima
Milasiute, Akvile
Vitkus, Dalius
Strupas, Kestutis
Gulla, Aiste
Sakinyte, Ieva
Razumiene, Julija
author_facet Budvytyte, Rima
Milasiute, Akvile
Vitkus, Dalius
Strupas, Kestutis
Gulla, Aiste
Sakinyte, Ieva
Razumiene, Julija
author_sort Budvytyte, Rima
collection PubMed
description Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.
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spelling pubmed-83013132021-07-24 Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis Budvytyte, Rima Milasiute, Akvile Vitkus, Dalius Strupas, Kestutis Gulla, Aiste Sakinyte, Ieva Razumiene, Julija Biomedicines Article Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity. MDPI 2021-06-29 /pmc/articles/PMC8301313/ /pubmed/34210023 http://dx.doi.org/10.3390/biomedicines9070755 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Budvytyte, Rima
Milasiute, Akvile
Vitkus, Dalius
Strupas, Kestutis
Gulla, Aiste
Sakinyte, Ieva
Razumiene, Julija
Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis
title Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis
title_full Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis
title_fullStr Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis
title_full_unstemmed Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis
title_short Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis
title_sort tethered lipid membranes as a nanoscale arrangement towards non-invasive analysis of acute pancreatitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301313/
https://www.ncbi.nlm.nih.gov/pubmed/34210023
http://dx.doi.org/10.3390/biomedicines9070755
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