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Circulating Extracellular Vesicle Proteins and MicroRNA Profiles in Subcortical and Cortical-Subcortical Ischaemic Stroke

In order to investigate the role of circulating extracellular vesicles (EVs), proteins, and microRNAs as damage and repair markers in ischaemic stroke depending on its topography, subcortical (SC), and cortical-subcortical (CSC) involvement, we quantified the total amount of EVs using an enzyme-link...

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Autores principales: Otero-Ortega, Laura, Alonso-López, Elisa, Pérez-Mato, María, Laso-García, Fernando, Gómez-de Frutos, Mari Carmen, Diekhorst, Luke, García-Bermejo, María Laura, Conde-Moreno, Elisa, Fuentes, Blanca, de Leciñana, María Alonso, Bravo, Susana B., Díez-Tejedor, Exuperio, Gutiérrez-Fernández, María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301391/
https://www.ncbi.nlm.nih.gov/pubmed/34356850
http://dx.doi.org/10.3390/biomedicines9070786
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author Otero-Ortega, Laura
Alonso-López, Elisa
Pérez-Mato, María
Laso-García, Fernando
Gómez-de Frutos, Mari Carmen
Diekhorst, Luke
García-Bermejo, María Laura
Conde-Moreno, Elisa
Fuentes, Blanca
de Leciñana, María Alonso
Bravo, Susana B.
Díez-Tejedor, Exuperio
Gutiérrez-Fernández, María
author_facet Otero-Ortega, Laura
Alonso-López, Elisa
Pérez-Mato, María
Laso-García, Fernando
Gómez-de Frutos, Mari Carmen
Diekhorst, Luke
García-Bermejo, María Laura
Conde-Moreno, Elisa
Fuentes, Blanca
de Leciñana, María Alonso
Bravo, Susana B.
Díez-Tejedor, Exuperio
Gutiérrez-Fernández, María
author_sort Otero-Ortega, Laura
collection PubMed
description In order to investigate the role of circulating extracellular vesicles (EVs), proteins, and microRNAs as damage and repair markers in ischaemic stroke depending on its topography, subcortical (SC), and cortical-subcortical (CSC) involvement, we quantified the total amount of EVs using an enzyme-linked immunosorbent assay technique and analysed their global protein content using proteomics. We also employed a polymerase chain reaction to evaluate the circulating microRNA profile. The study included 81 patients with ischaemic stroke (26 SC and 55 CSC) and 22 healthy controls (HCs). No differences were found in circulating EV levels between the SC, CSC, and HC groups. We detected the specific expression of C1QA and Casp14 in the EVs of patients with CSC ischaemic stroke and the specific expression of ANXA2 in the EVs of patients with SC involvement. Patients with CSC ischaemic stroke showed a lower expression of miR-15a, miR-424, miR-100, and miR-339 compared with those with SC ischaemic stroke, and the levels of miR-339, miR-100, miR-199a, miR-369a, miR-424, and miR-15a were lower than those of the HCs. Circulating EV proteins and microRNAs from patients with CSC ischaemic stroke could be considered markers of neurite outgrowth, neurogenesis, inflammation process, and atherosclerosis. On the other hand, EV proteins and microRNAs from patients with SC ischaemic stroke might be markers of an anti-inflammatory process and blood–brain barrier disruption reduction.
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spelling pubmed-83013912021-07-24 Circulating Extracellular Vesicle Proteins and MicroRNA Profiles in Subcortical and Cortical-Subcortical Ischaemic Stroke Otero-Ortega, Laura Alonso-López, Elisa Pérez-Mato, María Laso-García, Fernando Gómez-de Frutos, Mari Carmen Diekhorst, Luke García-Bermejo, María Laura Conde-Moreno, Elisa Fuentes, Blanca de Leciñana, María Alonso Bravo, Susana B. Díez-Tejedor, Exuperio Gutiérrez-Fernández, María Biomedicines Article In order to investigate the role of circulating extracellular vesicles (EVs), proteins, and microRNAs as damage and repair markers in ischaemic stroke depending on its topography, subcortical (SC), and cortical-subcortical (CSC) involvement, we quantified the total amount of EVs using an enzyme-linked immunosorbent assay technique and analysed their global protein content using proteomics. We also employed a polymerase chain reaction to evaluate the circulating microRNA profile. The study included 81 patients with ischaemic stroke (26 SC and 55 CSC) and 22 healthy controls (HCs). No differences were found in circulating EV levels between the SC, CSC, and HC groups. We detected the specific expression of C1QA and Casp14 in the EVs of patients with CSC ischaemic stroke and the specific expression of ANXA2 in the EVs of patients with SC involvement. Patients with CSC ischaemic stroke showed a lower expression of miR-15a, miR-424, miR-100, and miR-339 compared with those with SC ischaemic stroke, and the levels of miR-339, miR-100, miR-199a, miR-369a, miR-424, and miR-15a were lower than those of the HCs. Circulating EV proteins and microRNAs from patients with CSC ischaemic stroke could be considered markers of neurite outgrowth, neurogenesis, inflammation process, and atherosclerosis. On the other hand, EV proteins and microRNAs from patients with SC ischaemic stroke might be markers of an anti-inflammatory process and blood–brain barrier disruption reduction. MDPI 2021-07-07 /pmc/articles/PMC8301391/ /pubmed/34356850 http://dx.doi.org/10.3390/biomedicines9070786 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Otero-Ortega, Laura
Alonso-López, Elisa
Pérez-Mato, María
Laso-García, Fernando
Gómez-de Frutos, Mari Carmen
Diekhorst, Luke
García-Bermejo, María Laura
Conde-Moreno, Elisa
Fuentes, Blanca
de Leciñana, María Alonso
Bravo, Susana B.
Díez-Tejedor, Exuperio
Gutiérrez-Fernández, María
Circulating Extracellular Vesicle Proteins and MicroRNA Profiles in Subcortical and Cortical-Subcortical Ischaemic Stroke
title Circulating Extracellular Vesicle Proteins and MicroRNA Profiles in Subcortical and Cortical-Subcortical Ischaemic Stroke
title_full Circulating Extracellular Vesicle Proteins and MicroRNA Profiles in Subcortical and Cortical-Subcortical Ischaemic Stroke
title_fullStr Circulating Extracellular Vesicle Proteins and MicroRNA Profiles in Subcortical and Cortical-Subcortical Ischaemic Stroke
title_full_unstemmed Circulating Extracellular Vesicle Proteins and MicroRNA Profiles in Subcortical and Cortical-Subcortical Ischaemic Stroke
title_short Circulating Extracellular Vesicle Proteins and MicroRNA Profiles in Subcortical and Cortical-Subcortical Ischaemic Stroke
title_sort circulating extracellular vesicle proteins and microrna profiles in subcortical and cortical-subcortical ischaemic stroke
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301391/
https://www.ncbi.nlm.nih.gov/pubmed/34356850
http://dx.doi.org/10.3390/biomedicines9070786
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