Cargando…
Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients
Type 1 CD4(+) T helper (Th1) cells mediate resistance to Mycobacterium tuberculosis (Mtb), and Th2 immunity generates specific immunoglobulin E upon allergen exposure. We investigated the impact of active tuberculosis (TB), atopic status, and anti-TB treatment on the balance between Th1 and Th2 (typ...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301453/ https://www.ncbi.nlm.nih.gov/pubmed/34202662 http://dx.doi.org/10.3390/biomedicines9070724 |
_version_ | 1783726673389158400 |
---|---|
author | Lo, Chun-Yu Huang, Yu-Chen Huang, Hung-Yu Chung, Fu-Tsai Lin, Chang-Wei Chung, Kian Fan Wang, Chun-Hua |
author_facet | Lo, Chun-Yu Huang, Yu-Chen Huang, Hung-Yu Chung, Fu-Tsai Lin, Chang-Wei Chung, Kian Fan Wang, Chun-Hua |
author_sort | Lo, Chun-Yu |
collection | PubMed |
description | Type 1 CD4(+) T helper (Th1) cells mediate resistance to Mycobacterium tuberculosis (Mtb), and Th2 immunity generates specific immunoglobulin E upon allergen exposure. We investigated the impact of active tuberculosis (TB), atopic status, and anti-TB treatment on the balance between Th1 and Th2 (type 2 CD4(+) T helper) immunity. CD4(+)/interferon (IFN)-γ(+) Th1 cells (%Th1) and CD4(+)/interleukin-4(+) Th2 cells (%Th2) in bronchoalveolar lavage (BAL) fluid and peripheral blood mononuclear cells (PBMCs) were measured by flow cytometry. The BAL %Th1 was higher in TB patients at baseline, compared to that in non-TB subjects, and was further increased in TB patients after stimulation with phorbol myristate acetate and ionomycin. The stimulated BAL %Th1 was inversely correlated with the severity score of chest radiography in TB patients. Heat-killed Mtb triggered more IFN-γ and nitrite production, as determined by enzyme-linked immunosorbent assay and the Griess reaction, respectively, from the alveolar macrophages of TB patients than that of non-TB subjects. Non-atopic TB participants had a higher %Th1 in PBMCs, compared to atopic individuals, and their %Th1 decreased after 3-month anti-TB treatment. Th1 response is provoked by active TB infection, is associated with less severe radiographic changes, is reduced in atopic patients with active TB infection, and is attenuated after anti-TB treatment. |
format | Online Article Text |
id | pubmed-8301453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83014532021-07-24 Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients Lo, Chun-Yu Huang, Yu-Chen Huang, Hung-Yu Chung, Fu-Tsai Lin, Chang-Wei Chung, Kian Fan Wang, Chun-Hua Biomedicines Article Type 1 CD4(+) T helper (Th1) cells mediate resistance to Mycobacterium tuberculosis (Mtb), and Th2 immunity generates specific immunoglobulin E upon allergen exposure. We investigated the impact of active tuberculosis (TB), atopic status, and anti-TB treatment on the balance between Th1 and Th2 (type 2 CD4(+) T helper) immunity. CD4(+)/interferon (IFN)-γ(+) Th1 cells (%Th1) and CD4(+)/interleukin-4(+) Th2 cells (%Th2) in bronchoalveolar lavage (BAL) fluid and peripheral blood mononuclear cells (PBMCs) were measured by flow cytometry. The BAL %Th1 was higher in TB patients at baseline, compared to that in non-TB subjects, and was further increased in TB patients after stimulation with phorbol myristate acetate and ionomycin. The stimulated BAL %Th1 was inversely correlated with the severity score of chest radiography in TB patients. Heat-killed Mtb triggered more IFN-γ and nitrite production, as determined by enzyme-linked immunosorbent assay and the Griess reaction, respectively, from the alveolar macrophages of TB patients than that of non-TB subjects. Non-atopic TB participants had a higher %Th1 in PBMCs, compared to atopic individuals, and their %Th1 decreased after 3-month anti-TB treatment. Th1 response is provoked by active TB infection, is associated with less severe radiographic changes, is reduced in atopic patients with active TB infection, and is attenuated after anti-TB treatment. MDPI 2021-06-24 /pmc/articles/PMC8301453/ /pubmed/34202662 http://dx.doi.org/10.3390/biomedicines9070724 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lo, Chun-Yu Huang, Yu-Chen Huang, Hung-Yu Chung, Fu-Tsai Lin, Chang-Wei Chung, Kian Fan Wang, Chun-Hua Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients |
title | Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients |
title_full | Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients |
title_fullStr | Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients |
title_full_unstemmed | Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients |
title_short | Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients |
title_sort | increased th1 cells with disease resolution of active pulmonary tuberculosis in non-atopic patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301453/ https://www.ncbi.nlm.nih.gov/pubmed/34202662 http://dx.doi.org/10.3390/biomedicines9070724 |
work_keys_str_mv | AT lochunyu increasedth1cellswithdiseaseresolutionofactivepulmonarytuberculosisinnonatopicpatients AT huangyuchen increasedth1cellswithdiseaseresolutionofactivepulmonarytuberculosisinnonatopicpatients AT huanghungyu increasedth1cellswithdiseaseresolutionofactivepulmonarytuberculosisinnonatopicpatients AT chungfutsai increasedth1cellswithdiseaseresolutionofactivepulmonarytuberculosisinnonatopicpatients AT linchangwei increasedth1cellswithdiseaseresolutionofactivepulmonarytuberculosisinnonatopicpatients AT chungkianfan increasedth1cellswithdiseaseresolutionofactivepulmonarytuberculosisinnonatopicpatients AT wangchunhua increasedth1cellswithdiseaseresolutionofactivepulmonarytuberculosisinnonatopicpatients |