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Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients

Type 1 CD4(+) T helper (Th1) cells mediate resistance to Mycobacterium tuberculosis (Mtb), and Th2 immunity generates specific immunoglobulin E upon allergen exposure. We investigated the impact of active tuberculosis (TB), atopic status, and anti-TB treatment on the balance between Th1 and Th2 (typ...

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Autores principales: Lo, Chun-Yu, Huang, Yu-Chen, Huang, Hung-Yu, Chung, Fu-Tsai, Lin, Chang-Wei, Chung, Kian Fan, Wang, Chun-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301453/
https://www.ncbi.nlm.nih.gov/pubmed/34202662
http://dx.doi.org/10.3390/biomedicines9070724
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author Lo, Chun-Yu
Huang, Yu-Chen
Huang, Hung-Yu
Chung, Fu-Tsai
Lin, Chang-Wei
Chung, Kian Fan
Wang, Chun-Hua
author_facet Lo, Chun-Yu
Huang, Yu-Chen
Huang, Hung-Yu
Chung, Fu-Tsai
Lin, Chang-Wei
Chung, Kian Fan
Wang, Chun-Hua
author_sort Lo, Chun-Yu
collection PubMed
description Type 1 CD4(+) T helper (Th1) cells mediate resistance to Mycobacterium tuberculosis (Mtb), and Th2 immunity generates specific immunoglobulin E upon allergen exposure. We investigated the impact of active tuberculosis (TB), atopic status, and anti-TB treatment on the balance between Th1 and Th2 (type 2 CD4(+) T helper) immunity. CD4(+)/interferon (IFN)-γ(+) Th1 cells (%Th1) and CD4(+)/interleukin-4(+) Th2 cells (%Th2) in bronchoalveolar lavage (BAL) fluid and peripheral blood mononuclear cells (PBMCs) were measured by flow cytometry. The BAL %Th1 was higher in TB patients at baseline, compared to that in non-TB subjects, and was further increased in TB patients after stimulation with phorbol myristate acetate and ionomycin. The stimulated BAL %Th1 was inversely correlated with the severity score of chest radiography in TB patients. Heat-killed Mtb triggered more IFN-γ and nitrite production, as determined by enzyme-linked immunosorbent assay and the Griess reaction, respectively, from the alveolar macrophages of TB patients than that of non-TB subjects. Non-atopic TB participants had a higher %Th1 in PBMCs, compared to atopic individuals, and their %Th1 decreased after 3-month anti-TB treatment. Th1 response is provoked by active TB infection, is associated with less severe radiographic changes, is reduced in atopic patients with active TB infection, and is attenuated after anti-TB treatment.
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spelling pubmed-83014532021-07-24 Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients Lo, Chun-Yu Huang, Yu-Chen Huang, Hung-Yu Chung, Fu-Tsai Lin, Chang-Wei Chung, Kian Fan Wang, Chun-Hua Biomedicines Article Type 1 CD4(+) T helper (Th1) cells mediate resistance to Mycobacterium tuberculosis (Mtb), and Th2 immunity generates specific immunoglobulin E upon allergen exposure. We investigated the impact of active tuberculosis (TB), atopic status, and anti-TB treatment on the balance between Th1 and Th2 (type 2 CD4(+) T helper) immunity. CD4(+)/interferon (IFN)-γ(+) Th1 cells (%Th1) and CD4(+)/interleukin-4(+) Th2 cells (%Th2) in bronchoalveolar lavage (BAL) fluid and peripheral blood mononuclear cells (PBMCs) were measured by flow cytometry. The BAL %Th1 was higher in TB patients at baseline, compared to that in non-TB subjects, and was further increased in TB patients after stimulation with phorbol myristate acetate and ionomycin. The stimulated BAL %Th1 was inversely correlated with the severity score of chest radiography in TB patients. Heat-killed Mtb triggered more IFN-γ and nitrite production, as determined by enzyme-linked immunosorbent assay and the Griess reaction, respectively, from the alveolar macrophages of TB patients than that of non-TB subjects. Non-atopic TB participants had a higher %Th1 in PBMCs, compared to atopic individuals, and their %Th1 decreased after 3-month anti-TB treatment. Th1 response is provoked by active TB infection, is associated with less severe radiographic changes, is reduced in atopic patients with active TB infection, and is attenuated after anti-TB treatment. MDPI 2021-06-24 /pmc/articles/PMC8301453/ /pubmed/34202662 http://dx.doi.org/10.3390/biomedicines9070724 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lo, Chun-Yu
Huang, Yu-Chen
Huang, Hung-Yu
Chung, Fu-Tsai
Lin, Chang-Wei
Chung, Kian Fan
Wang, Chun-Hua
Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients
title Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients
title_full Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients
title_fullStr Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients
title_full_unstemmed Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients
title_short Increased Th1 Cells with Disease Resolution of Active Pulmonary Tuberculosis in Non-Atopic Patients
title_sort increased th1 cells with disease resolution of active pulmonary tuberculosis in non-atopic patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301453/
https://www.ncbi.nlm.nih.gov/pubmed/34202662
http://dx.doi.org/10.3390/biomedicines9070724
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