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Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma

Esophageal cancer is one of the most aggressive malignant neoplasms, with low survival rates and limited treatment options. In this study we analyzed the microbiome composition and the phenotype of inflammatory tumor infiltrate in squamous cell carcinoma of esophagus (ESCC) and examined possible rel...

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Autores principales: Kovaleva, Olga, Podlesnaya, Polina, Rashidova, Madina, Samoilova, Daria, Petrenko, Anatoly, Mochalnikova, Valeria, Kataev, Vladimir, Khlopko, Yuri, Plotnikov, Andrey, Gratchev, Alexei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301468/
https://www.ncbi.nlm.nih.gov/pubmed/34203319
http://dx.doi.org/10.3390/biomedicines9070743
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author Kovaleva, Olga
Podlesnaya, Polina
Rashidova, Madina
Samoilova, Daria
Petrenko, Anatoly
Mochalnikova, Valeria
Kataev, Vladimir
Khlopko, Yuri
Plotnikov, Andrey
Gratchev, Alexei
author_facet Kovaleva, Olga
Podlesnaya, Polina
Rashidova, Madina
Samoilova, Daria
Petrenko, Anatoly
Mochalnikova, Valeria
Kataev, Vladimir
Khlopko, Yuri
Plotnikov, Andrey
Gratchev, Alexei
author_sort Kovaleva, Olga
collection PubMed
description Esophageal cancer is one of the most aggressive malignant neoplasms, with low survival rates and limited treatment options. In this study we analyzed the microbiome composition and the phenotype of inflammatory tumor infiltrate in squamous cell carcinoma of esophagus (ESCC) and examined possible relationships between them and their prognostic significance. We found that the predominant phyla of microorganisms found in both tumors and adjacent normal tissues were Firmicutes, Proteobacteria, Actinobacteria, Gemmatimonadetes and Bacteroidetes. We established that only bacteria of the genus Staphylococcus differ between tumors and normal tissues. We found a significant correlation between bacterial burden and the phenotype of the tumor stroma. Namely, a group of tumors characterized by a high expression of CD206 (r = −0.3976, p = 0.0056) in the stroma and iNOS (r = −0.2953, p = 0.0439) in tumor cells is characterized by a higher bacterial burden. Further, we established that in the group with a high content of CD206+ macrophages, there is also a predominance of gram-positive bacteria over gram-negative ones. We found that gram-positive bacterial burden is associated with disease prognosis in ESCC showing high content of CD206+ macrophages. In conclusion we established that the tumor microbiome, can be prognostically significant for ESCC when combined with other stromal markers.
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spelling pubmed-83014682021-07-24 Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma Kovaleva, Olga Podlesnaya, Polina Rashidova, Madina Samoilova, Daria Petrenko, Anatoly Mochalnikova, Valeria Kataev, Vladimir Khlopko, Yuri Plotnikov, Andrey Gratchev, Alexei Biomedicines Article Esophageal cancer is one of the most aggressive malignant neoplasms, with low survival rates and limited treatment options. In this study we analyzed the microbiome composition and the phenotype of inflammatory tumor infiltrate in squamous cell carcinoma of esophagus (ESCC) and examined possible relationships between them and their prognostic significance. We found that the predominant phyla of microorganisms found in both tumors and adjacent normal tissues were Firmicutes, Proteobacteria, Actinobacteria, Gemmatimonadetes and Bacteroidetes. We established that only bacteria of the genus Staphylococcus differ between tumors and normal tissues. We found a significant correlation between bacterial burden and the phenotype of the tumor stroma. Namely, a group of tumors characterized by a high expression of CD206 (r = −0.3976, p = 0.0056) in the stroma and iNOS (r = −0.2953, p = 0.0439) in tumor cells is characterized by a higher bacterial burden. Further, we established that in the group with a high content of CD206+ macrophages, there is also a predominance of gram-positive bacteria over gram-negative ones. We found that gram-positive bacterial burden is associated with disease prognosis in ESCC showing high content of CD206+ macrophages. In conclusion we established that the tumor microbiome, can be prognostically significant for ESCC when combined with other stromal markers. MDPI 2021-06-28 /pmc/articles/PMC8301468/ /pubmed/34203319 http://dx.doi.org/10.3390/biomedicines9070743 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kovaleva, Olga
Podlesnaya, Polina
Rashidova, Madina
Samoilova, Daria
Petrenko, Anatoly
Mochalnikova, Valeria
Kataev, Vladimir
Khlopko, Yuri
Plotnikov, Andrey
Gratchev, Alexei
Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma
title Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma
title_full Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma
title_fullStr Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma
title_full_unstemmed Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma
title_short Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma
title_sort prognostic significance of the microbiome and stromal cells phenotype in esophagus squamous cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301468/
https://www.ncbi.nlm.nih.gov/pubmed/34203319
http://dx.doi.org/10.3390/biomedicines9070743
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