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miR-208b Reduces the Expression of Kcnj5 in a Cardiomyocyte Cell Line
MicroRNAs (miRs) contribute to different aspects of cardiovascular pathology, among them cardiac hypertrophy and atrial fibrillation. Cardiac miR expression was analyzed in a mouse model with structural and electrical remodeling. Next-generation sequencing revealed that miR-208b-3p was ~25-fold upre...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301481/ https://www.ncbi.nlm.nih.gov/pubmed/34201741 http://dx.doi.org/10.3390/biomedicines9070719 |
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author | Hupfeld, Julia Ernst, Maximilian Knyrim, Maria Binas, Stephanie Kloeckner, Udo Rabe, Sindy Quarch, Katja Misiak, Danny Fuszard, Matthew Grossmann, Claudia Gekle, Michael Schreier, Barbara |
author_facet | Hupfeld, Julia Ernst, Maximilian Knyrim, Maria Binas, Stephanie Kloeckner, Udo Rabe, Sindy Quarch, Katja Misiak, Danny Fuszard, Matthew Grossmann, Claudia Gekle, Michael Schreier, Barbara |
author_sort | Hupfeld, Julia |
collection | PubMed |
description | MicroRNAs (miRs) contribute to different aspects of cardiovascular pathology, among them cardiac hypertrophy and atrial fibrillation. Cardiac miR expression was analyzed in a mouse model with structural and electrical remodeling. Next-generation sequencing revealed that miR-208b-3p was ~25-fold upregulated. Therefore, the aim of our study was to evaluate the impact of miR-208b on cardiac protein expression. First, an undirected approach comparing whole RNA sequencing data to miR-walk 2.0 miR-208b 3′-UTR targets revealed 58 potential targets of miR-208b being regulated. We were able to show that miR-208b mimics bind to the 3′ untranslated region (UTR) of voltage-gated calcium channel subunit alpha1 C and Kcnj5, two predicted targets of miR-208b. Additionally, we demonstrated that miR-208b mimics reduce GIRK1/4 channel-dependent thallium ion flux in HL-1 cells. In a second undirected approach we performed mass spectrometry to identify the potential targets of miR-208b. We identified 40 potential targets by comparison to miR-walk 2.0 3′-UTR, 5′-UTR and CDS targets. Among those targets, Rock2 and Ran were upregulated in Western blots of HL-1 cells by miR-208b mimics. In summary, miR-208b targets the mRNAs of proteins involved in the generation of cardiac excitation and propagation, as well as of proteins involved in RNA translocation (Ran) and cardiac hypertrophic response (Rock2). |
format | Online Article Text |
id | pubmed-8301481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83014812021-07-24 miR-208b Reduces the Expression of Kcnj5 in a Cardiomyocyte Cell Line Hupfeld, Julia Ernst, Maximilian Knyrim, Maria Binas, Stephanie Kloeckner, Udo Rabe, Sindy Quarch, Katja Misiak, Danny Fuszard, Matthew Grossmann, Claudia Gekle, Michael Schreier, Barbara Biomedicines Article MicroRNAs (miRs) contribute to different aspects of cardiovascular pathology, among them cardiac hypertrophy and atrial fibrillation. Cardiac miR expression was analyzed in a mouse model with structural and electrical remodeling. Next-generation sequencing revealed that miR-208b-3p was ~25-fold upregulated. Therefore, the aim of our study was to evaluate the impact of miR-208b on cardiac protein expression. First, an undirected approach comparing whole RNA sequencing data to miR-walk 2.0 miR-208b 3′-UTR targets revealed 58 potential targets of miR-208b being regulated. We were able to show that miR-208b mimics bind to the 3′ untranslated region (UTR) of voltage-gated calcium channel subunit alpha1 C and Kcnj5, two predicted targets of miR-208b. Additionally, we demonstrated that miR-208b mimics reduce GIRK1/4 channel-dependent thallium ion flux in HL-1 cells. In a second undirected approach we performed mass spectrometry to identify the potential targets of miR-208b. We identified 40 potential targets by comparison to miR-walk 2.0 3′-UTR, 5′-UTR and CDS targets. Among those targets, Rock2 and Ran were upregulated in Western blots of HL-1 cells by miR-208b mimics. In summary, miR-208b targets the mRNAs of proteins involved in the generation of cardiac excitation and propagation, as well as of proteins involved in RNA translocation (Ran) and cardiac hypertrophic response (Rock2). MDPI 2021-06-23 /pmc/articles/PMC8301481/ /pubmed/34201741 http://dx.doi.org/10.3390/biomedicines9070719 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hupfeld, Julia Ernst, Maximilian Knyrim, Maria Binas, Stephanie Kloeckner, Udo Rabe, Sindy Quarch, Katja Misiak, Danny Fuszard, Matthew Grossmann, Claudia Gekle, Michael Schreier, Barbara miR-208b Reduces the Expression of Kcnj5 in a Cardiomyocyte Cell Line |
title | miR-208b Reduces the Expression of Kcnj5 in a Cardiomyocyte Cell Line |
title_full | miR-208b Reduces the Expression of Kcnj5 in a Cardiomyocyte Cell Line |
title_fullStr | miR-208b Reduces the Expression of Kcnj5 in a Cardiomyocyte Cell Line |
title_full_unstemmed | miR-208b Reduces the Expression of Kcnj5 in a Cardiomyocyte Cell Line |
title_short | miR-208b Reduces the Expression of Kcnj5 in a Cardiomyocyte Cell Line |
title_sort | mir-208b reduces the expression of kcnj5 in a cardiomyocyte cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301481/ https://www.ncbi.nlm.nih.gov/pubmed/34201741 http://dx.doi.org/10.3390/biomedicines9070719 |
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