Cargando…
Enrichment of leukocytes in peripheral blood using 3D printed tubes
Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301617/ https://www.ncbi.nlm.nih.gov/pubmed/34297742 http://dx.doi.org/10.1371/journal.pone.0254615 |
_version_ | 1783726710777184256 |
---|---|
author | Guo, Li-fang Wang, Liu Ren, Sai Su, Ning Wei, Kun Sun, Xian-Ge Ren, Xiao-Dong Huang, Qing |
author_facet | Guo, Li-fang Wang, Liu Ren, Sai Su, Ning Wei, Kun Sun, Xian-Ge Ren, Xiao-Dong Huang, Qing |
author_sort | Guo, Li-fang |
collection | PubMed |
description | Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure label-free initial leukocyte enrichment based on cell density and size. The internal structure of leukocyte sorting accumulator (revealed here in a new design, leukocyte sorting accumulator-3, upgraded from earlier models), optimizes localization of the buffy coat fraction and the length of the period allocated for a second centrifugation step to deliver a higher recovery of buffy coats than earlier models. Established methodological parameters were evaluated for reliability by calculating leukocyte recovery rates and erythrocyte depletion rates by both pushing and pulling methods of cell displacement. Results indicate that leukocyte sorting accumulator-3 achieves a mean leukocytes recovery fraction of 96.2 ± 2.38% by the pushing method of layer displacement. By the pulling method, the leukocyte sorting accumulator-3 yield a mean leukocytes recovery fraction of 94.4 ± 0.8%. New procedures for preliminary enrichment of leukocytes from peripheral blood that avoid cellular damage, as well as avert metabolic and phase cycle intervention, are required as the first step in many modern clinical and basic research assays. |
format | Online Article Text |
id | pubmed-8301617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-83016172021-07-31 Enrichment of leukocytes in peripheral blood using 3D printed tubes Guo, Li-fang Wang, Liu Ren, Sai Su, Ning Wei, Kun Sun, Xian-Ge Ren, Xiao-Dong Huang, Qing PLoS One Research Article Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure label-free initial leukocyte enrichment based on cell density and size. The internal structure of leukocyte sorting accumulator (revealed here in a new design, leukocyte sorting accumulator-3, upgraded from earlier models), optimizes localization of the buffy coat fraction and the length of the period allocated for a second centrifugation step to deliver a higher recovery of buffy coats than earlier models. Established methodological parameters were evaluated for reliability by calculating leukocyte recovery rates and erythrocyte depletion rates by both pushing and pulling methods of cell displacement. Results indicate that leukocyte sorting accumulator-3 achieves a mean leukocytes recovery fraction of 96.2 ± 2.38% by the pushing method of layer displacement. By the pulling method, the leukocyte sorting accumulator-3 yield a mean leukocytes recovery fraction of 94.4 ± 0.8%. New procedures for preliminary enrichment of leukocytes from peripheral blood that avoid cellular damage, as well as avert metabolic and phase cycle intervention, are required as the first step in many modern clinical and basic research assays. Public Library of Science 2021-07-23 /pmc/articles/PMC8301617/ /pubmed/34297742 http://dx.doi.org/10.1371/journal.pone.0254615 Text en © 2021 Guo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Guo, Li-fang Wang, Liu Ren, Sai Su, Ning Wei, Kun Sun, Xian-Ge Ren, Xiao-Dong Huang, Qing Enrichment of leukocytes in peripheral blood using 3D printed tubes |
title | Enrichment of leukocytes in peripheral blood using 3D printed tubes |
title_full | Enrichment of leukocytes in peripheral blood using 3D printed tubes |
title_fullStr | Enrichment of leukocytes in peripheral blood using 3D printed tubes |
title_full_unstemmed | Enrichment of leukocytes in peripheral blood using 3D printed tubes |
title_short | Enrichment of leukocytes in peripheral blood using 3D printed tubes |
title_sort | enrichment of leukocytes in peripheral blood using 3d printed tubes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301617/ https://www.ncbi.nlm.nih.gov/pubmed/34297742 http://dx.doi.org/10.1371/journal.pone.0254615 |
work_keys_str_mv | AT guolifang enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT wangliu enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT rensai enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT suning enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT weikun enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT sunxiange enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT renxiaodong enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT huangqing enrichmentofleukocytesinperipheralbloodusing3dprintedtubes |