Cargando…

Enrichment of leukocytes in peripheral blood using 3D printed tubes

Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Li-fang, Wang, Liu, Ren, Sai, Su, Ning, Wei, Kun, Sun, Xian-Ge, Ren, Xiao-Dong, Huang, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301617/
https://www.ncbi.nlm.nih.gov/pubmed/34297742
http://dx.doi.org/10.1371/journal.pone.0254615
_version_ 1783726710777184256
author Guo, Li-fang
Wang, Liu
Ren, Sai
Su, Ning
Wei, Kun
Sun, Xian-Ge
Ren, Xiao-Dong
Huang, Qing
author_facet Guo, Li-fang
Wang, Liu
Ren, Sai
Su, Ning
Wei, Kun
Sun, Xian-Ge
Ren, Xiao-Dong
Huang, Qing
author_sort Guo, Li-fang
collection PubMed
description Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure label-free initial leukocyte enrichment based on cell density and size. The internal structure of leukocyte sorting accumulator (revealed here in a new design, leukocyte sorting accumulator-3, upgraded from earlier models), optimizes localization of the buffy coat fraction and the length of the period allocated for a second centrifugation step to deliver a higher recovery of buffy coats than earlier models. Established methodological parameters were evaluated for reliability by calculating leukocyte recovery rates and erythrocyte depletion rates by both pushing and pulling methods of cell displacement. Results indicate that leukocyte sorting accumulator-3 achieves a mean leukocytes recovery fraction of 96.2 ± 2.38% by the pushing method of layer displacement. By the pulling method, the leukocyte sorting accumulator-3 yield a mean leukocytes recovery fraction of 94.4 ± 0.8%. New procedures for preliminary enrichment of leukocytes from peripheral blood that avoid cellular damage, as well as avert metabolic and phase cycle intervention, are required as the first step in many modern clinical and basic research assays.
format Online
Article
Text
id pubmed-8301617
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-83016172021-07-31 Enrichment of leukocytes in peripheral blood using 3D printed tubes Guo, Li-fang Wang, Liu Ren, Sai Su, Ning Wei, Kun Sun, Xian-Ge Ren, Xiao-Dong Huang, Qing PLoS One Research Article Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure label-free initial leukocyte enrichment based on cell density and size. The internal structure of leukocyte sorting accumulator (revealed here in a new design, leukocyte sorting accumulator-3, upgraded from earlier models), optimizes localization of the buffy coat fraction and the length of the period allocated for a second centrifugation step to deliver a higher recovery of buffy coats than earlier models. Established methodological parameters were evaluated for reliability by calculating leukocyte recovery rates and erythrocyte depletion rates by both pushing and pulling methods of cell displacement. Results indicate that leukocyte sorting accumulator-3 achieves a mean leukocytes recovery fraction of 96.2 ± 2.38% by the pushing method of layer displacement. By the pulling method, the leukocyte sorting accumulator-3 yield a mean leukocytes recovery fraction of 94.4 ± 0.8%. New procedures for preliminary enrichment of leukocytes from peripheral blood that avoid cellular damage, as well as avert metabolic and phase cycle intervention, are required as the first step in many modern clinical and basic research assays. Public Library of Science 2021-07-23 /pmc/articles/PMC8301617/ /pubmed/34297742 http://dx.doi.org/10.1371/journal.pone.0254615 Text en © 2021 Guo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Guo, Li-fang
Wang, Liu
Ren, Sai
Su, Ning
Wei, Kun
Sun, Xian-Ge
Ren, Xiao-Dong
Huang, Qing
Enrichment of leukocytes in peripheral blood using 3D printed tubes
title Enrichment of leukocytes in peripheral blood using 3D printed tubes
title_full Enrichment of leukocytes in peripheral blood using 3D printed tubes
title_fullStr Enrichment of leukocytes in peripheral blood using 3D printed tubes
title_full_unstemmed Enrichment of leukocytes in peripheral blood using 3D printed tubes
title_short Enrichment of leukocytes in peripheral blood using 3D printed tubes
title_sort enrichment of leukocytes in peripheral blood using 3d printed tubes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301617/
https://www.ncbi.nlm.nih.gov/pubmed/34297742
http://dx.doi.org/10.1371/journal.pone.0254615
work_keys_str_mv AT guolifang enrichmentofleukocytesinperipheralbloodusing3dprintedtubes
AT wangliu enrichmentofleukocytesinperipheralbloodusing3dprintedtubes
AT rensai enrichmentofleukocytesinperipheralbloodusing3dprintedtubes
AT suning enrichmentofleukocytesinperipheralbloodusing3dprintedtubes
AT weikun enrichmentofleukocytesinperipheralbloodusing3dprintedtubes
AT sunxiange enrichmentofleukocytesinperipheralbloodusing3dprintedtubes
AT renxiaodong enrichmentofleukocytesinperipheralbloodusing3dprintedtubes
AT huangqing enrichmentofleukocytesinperipheralbloodusing3dprintedtubes