Introduction to Single-Cell DNA Methylation Profiling Methods

DNA methylation is an epigenetic mechanism that is related to mammalian cellular differentiation, gene expression regulation, and disease. In several studies, DNA methylation has been identified as an effective marker to identify differences between cells. In this review, we introduce single-cell DN...

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Detalles Bibliográficos
Autores principales: Ahn, Jongseong, Heo, Sunghoon, Lee, Jihyun, Bang, Duhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301785/
https://www.ncbi.nlm.nih.gov/pubmed/34356635
http://dx.doi.org/10.3390/biom11071013
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author Ahn, Jongseong
Heo, Sunghoon
Lee, Jihyun
Bang, Duhee
author_facet Ahn, Jongseong
Heo, Sunghoon
Lee, Jihyun
Bang, Duhee
author_sort Ahn, Jongseong
collection PubMed
description DNA methylation is an epigenetic mechanism that is related to mammalian cellular differentiation, gene expression regulation, and disease. In several studies, DNA methylation has been identified as an effective marker to identify differences between cells. In this review, we introduce single-cell DNA-methylation profiling methods, including experimental strategies and approaches to computational data analysis. Furthermore, the blind spots of the basic analysis and recent alternatives are briefly described. In addition, we introduce well-known applications and discuss future development.
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spelling pubmed-83017852021-07-24 Introduction to Single-Cell DNA Methylation Profiling Methods Ahn, Jongseong Heo, Sunghoon Lee, Jihyun Bang, Duhee Biomolecules Review DNA methylation is an epigenetic mechanism that is related to mammalian cellular differentiation, gene expression regulation, and disease. In several studies, DNA methylation has been identified as an effective marker to identify differences between cells. In this review, we introduce single-cell DNA-methylation profiling methods, including experimental strategies and approaches to computational data analysis. Furthermore, the blind spots of the basic analysis and recent alternatives are briefly described. In addition, we introduce well-known applications and discuss future development. MDPI 2021-07-10 /pmc/articles/PMC8301785/ /pubmed/34356635 http://dx.doi.org/10.3390/biom11071013 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ahn, Jongseong
Heo, Sunghoon
Lee, Jihyun
Bang, Duhee
Introduction to Single-Cell DNA Methylation Profiling Methods
title Introduction to Single-Cell DNA Methylation Profiling Methods
title_full Introduction to Single-Cell DNA Methylation Profiling Methods
title_fullStr Introduction to Single-Cell DNA Methylation Profiling Methods
title_full_unstemmed Introduction to Single-Cell DNA Methylation Profiling Methods
title_short Introduction to Single-Cell DNA Methylation Profiling Methods
title_sort introduction to single-cell dna methylation profiling methods
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301785/
https://www.ncbi.nlm.nih.gov/pubmed/34356635
http://dx.doi.org/10.3390/biom11071013
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