DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids

Here, we report the controlled assembly of SWCNT–GFP hybrids employing DNA as a linker. Two distinct, enriched SWCNTs chiralities, (6,5), (7,6), and an unsorted SWCNT solution, were selectively functionalized with DNA and hybridized to a complementary GFP(DNA) conjugate. Atomic force microscopy imag...

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Detalles Bibliográficos
Autores principales: Freeley, Mark, Gwyther, Rebecca E. A., Jones, D. Dafydd, Palma, Matteo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301810/
https://www.ncbi.nlm.nih.gov/pubmed/34209628
http://dx.doi.org/10.3390/biom11070955
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author Freeley, Mark
Gwyther, Rebecca E. A.
Jones, D. Dafydd
Palma, Matteo
author_facet Freeley, Mark
Gwyther, Rebecca E. A.
Jones, D. Dafydd
Palma, Matteo
author_sort Freeley, Mark
collection PubMed
description Here, we report the controlled assembly of SWCNT–GFP hybrids employing DNA as a linker. Two distinct, enriched SWCNTs chiralities, (6,5), (7,6), and an unsorted SWCNT solution, were selectively functionalized with DNA and hybridized to a complementary GFP(DNA) conjugate. Atomic force microscopy images confirmed that GFP attachment occurred predominantly at the terminal ends of the nanotubes, as designed. The electronic coupling of the proteins to the nanotubes was confirmed via in-solution fluorescence spectroscopy, that revealed an increase in the emission intensity of GFP when linked to the CNTs.
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spelling pubmed-83018102021-07-24 DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids Freeley, Mark Gwyther, Rebecca E. A. Jones, D. Dafydd Palma, Matteo Biomolecules Communication Here, we report the controlled assembly of SWCNT–GFP hybrids employing DNA as a linker. Two distinct, enriched SWCNTs chiralities, (6,5), (7,6), and an unsorted SWCNT solution, were selectively functionalized with DNA and hybridized to a complementary GFP(DNA) conjugate. Atomic force microscopy images confirmed that GFP attachment occurred predominantly at the terminal ends of the nanotubes, as designed. The electronic coupling of the proteins to the nanotubes was confirmed via in-solution fluorescence spectroscopy, that revealed an increase in the emission intensity of GFP when linked to the CNTs. MDPI 2021-06-29 /pmc/articles/PMC8301810/ /pubmed/34209628 http://dx.doi.org/10.3390/biom11070955 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Freeley, Mark
Gwyther, Rebecca E. A.
Jones, D. Dafydd
Palma, Matteo
DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids
title DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids
title_full DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids
title_fullStr DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids
title_full_unstemmed DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids
title_short DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids
title_sort dna-directed assembly of carbon nanotube–protein hybrids
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301810/
https://www.ncbi.nlm.nih.gov/pubmed/34209628
http://dx.doi.org/10.3390/biom11070955
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AT palmamatteo dnadirectedassemblyofcarbonnanotubeproteinhybrids

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