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Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease
We report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301914/ https://www.ncbi.nlm.nih.gov/pubmed/34356688 http://dx.doi.org/10.3390/bios11070217 |
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author | Li, Dingdong Odessey, Rachel Li, Dongfang Pacifici, Domenico |
author_facet | Li, Dingdong Odessey, Rachel Li, Dongfang Pacifici, Domenico |
author_sort | Li, Dingdong |
collection | PubMed |
description | We report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization scheme to design, fabricate, and assess a biochemical sensing platform based on plasmonic interferometry that is able to detect physiological concentrations of TREM2 in solution. These findings open up opportunities for label-free biosensing of TREM2 in its soluble form in various bodily fluids as an early indicator of the onset of clinical dementia in AD. We also show that plasmonic interferometry can be a powerful tool to monitor and optimize surface immobilization schemes, which could be applied to develop other relevant antibody tests. |
format | Online Article Text |
id | pubmed-8301914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83019142021-07-24 Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease Li, Dingdong Odessey, Rachel Li, Dongfang Pacifici, Domenico Biosensors (Basel) Article We report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization scheme to design, fabricate, and assess a biochemical sensing platform based on plasmonic interferometry that is able to detect physiological concentrations of TREM2 in solution. These findings open up opportunities for label-free biosensing of TREM2 in its soluble form in various bodily fluids as an early indicator of the onset of clinical dementia in AD. We also show that plasmonic interferometry can be a powerful tool to monitor and optimize surface immobilization schemes, which could be applied to develop other relevant antibody tests. MDPI 2021-07-01 /pmc/articles/PMC8301914/ /pubmed/34356688 http://dx.doi.org/10.3390/bios11070217 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Dingdong Odessey, Rachel Li, Dongfang Pacifici, Domenico Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
title | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
title_full | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
title_fullStr | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
title_full_unstemmed | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
title_short | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
title_sort | plasmonic interferometers as trem2 sensors for alzheimer’s disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301914/ https://www.ncbi.nlm.nih.gov/pubmed/34356688 http://dx.doi.org/10.3390/bios11070217 |
work_keys_str_mv | AT lidingdong plasmonicinterferometersastrem2sensorsforalzheimersdisease AT odesseyrachel plasmonicinterferometersastrem2sensorsforalzheimersdisease AT lidongfang plasmonicinterferometersastrem2sensorsforalzheimersdisease AT pacificidomenico plasmonicinterferometersastrem2sensorsforalzheimersdisease |