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Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease

We report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization...

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Detalles Bibliográficos
Autores principales: Li, Dingdong, Odessey, Rachel, Li, Dongfang, Pacifici, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301914/
https://www.ncbi.nlm.nih.gov/pubmed/34356688
http://dx.doi.org/10.3390/bios11070217
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author Li, Dingdong
Odessey, Rachel
Li, Dongfang
Pacifici, Domenico
author_facet Li, Dingdong
Odessey, Rachel
Li, Dongfang
Pacifici, Domenico
author_sort Li, Dingdong
collection PubMed
description We report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization scheme to design, fabricate, and assess a biochemical sensing platform based on plasmonic interferometry that is able to detect physiological concentrations of TREM2 in solution. These findings open up opportunities for label-free biosensing of TREM2 in its soluble form in various bodily fluids as an early indicator of the onset of clinical dementia in AD. We also show that plasmonic interferometry can be a powerful tool to monitor and optimize surface immobilization schemes, which could be applied to develop other relevant antibody tests.
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spelling pubmed-83019142021-07-24 Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease Li, Dingdong Odessey, Rachel Li, Dongfang Pacifici, Domenico Biosensors (Basel) Article We report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization scheme to design, fabricate, and assess a biochemical sensing platform based on plasmonic interferometry that is able to detect physiological concentrations of TREM2 in solution. These findings open up opportunities for label-free biosensing of TREM2 in its soluble form in various bodily fluids as an early indicator of the onset of clinical dementia in AD. We also show that plasmonic interferometry can be a powerful tool to monitor and optimize surface immobilization schemes, which could be applied to develop other relevant antibody tests. MDPI 2021-07-01 /pmc/articles/PMC8301914/ /pubmed/34356688 http://dx.doi.org/10.3390/bios11070217 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Dingdong
Odessey, Rachel
Li, Dongfang
Pacifici, Domenico
Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease
title Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease
title_full Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease
title_fullStr Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease
title_full_unstemmed Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease
title_short Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease
title_sort plasmonic interferometers as trem2 sensors for alzheimer’s disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301914/
https://www.ncbi.nlm.nih.gov/pubmed/34356688
http://dx.doi.org/10.3390/bios11070217
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AT lidongfang plasmonicinterferometersastrem2sensorsforalzheimersdisease
AT pacificidomenico plasmonicinterferometersastrem2sensorsforalzheimersdisease