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Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men

Aging causes oxidative stress, endothelial dysfunction and a reduction in the bioavailability of nitric oxide. The study aim was to determine whether, as a result of repeated whole-body exposure to cryogenic temperature (3 min −130 °C), there is an increase of inducible nitric oxide synthase (iNOS)...

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Autores principales: Wiecek, Magdalena, Szygula, Zbigniew, Gradek, Joanna, Kusmierczyk, Justyna, Szymura, Jadwiga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301999/
https://www.ncbi.nlm.nih.gov/pubmed/34356664
http://dx.doi.org/10.3390/biom11071041
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author Wiecek, Magdalena
Szygula, Zbigniew
Gradek, Joanna
Kusmierczyk, Justyna
Szymura, Jadwiga
author_facet Wiecek, Magdalena
Szygula, Zbigniew
Gradek, Joanna
Kusmierczyk, Justyna
Szymura, Jadwiga
author_sort Wiecek, Magdalena
collection PubMed
description Aging causes oxidative stress, endothelial dysfunction and a reduction in the bioavailability of nitric oxide. The study aim was to determine whether, as a result of repeated whole-body exposure to cryogenic temperature (3 min −130 °C), there is an increase of inducible nitric oxide synthase (iNOS) concentration in senior subjects (59 ± 6 years), and if this effect is stronger in athletes. In 10 long-distance runners (RUN) and 10 untraining (UTR) men, 24 whole-body cryotherapy (WBC) procedures were performed. Prior to WBC, after 12th and 24th treatments and 7 days later, the concentration of iNOS, asymmetric dimethylarginine (ADMA), 3-nitrotyrosine (3-NTR), homocysteine (HCY), C-reactive protein (CRP) and interleukins such as: IL-6, IL-1β, IL-10 were measured. In the RUN and UTR groups, after 24 WBC, iNOS concentration was found to be comparable and significantly higher (F = 5.95, p < 0.01) (large clinical effect size) compared to before 1st WBC and after 12th WBC sessions. There were no changes in the concentration of the remaining markers as a result of WBC (p > 0.05). As a result of applying 24 WBC treatments, using the every-other-day model, iNOS concentration increased in the group of older men, regardless of their physical activity level. Along with this increase, there were no changes in nitro-oxidative stress or inflammation marker levels.
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spelling pubmed-83019992021-07-24 Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men Wiecek, Magdalena Szygula, Zbigniew Gradek, Joanna Kusmierczyk, Justyna Szymura, Jadwiga Biomolecules Article Aging causes oxidative stress, endothelial dysfunction and a reduction in the bioavailability of nitric oxide. The study aim was to determine whether, as a result of repeated whole-body exposure to cryogenic temperature (3 min −130 °C), there is an increase of inducible nitric oxide synthase (iNOS) concentration in senior subjects (59 ± 6 years), and if this effect is stronger in athletes. In 10 long-distance runners (RUN) and 10 untraining (UTR) men, 24 whole-body cryotherapy (WBC) procedures were performed. Prior to WBC, after 12th and 24th treatments and 7 days later, the concentration of iNOS, asymmetric dimethylarginine (ADMA), 3-nitrotyrosine (3-NTR), homocysteine (HCY), C-reactive protein (CRP) and interleukins such as: IL-6, IL-1β, IL-10 were measured. In the RUN and UTR groups, after 24 WBC, iNOS concentration was found to be comparable and significantly higher (F = 5.95, p < 0.01) (large clinical effect size) compared to before 1st WBC and after 12th WBC sessions. There were no changes in the concentration of the remaining markers as a result of WBC (p > 0.05). As a result of applying 24 WBC treatments, using the every-other-day model, iNOS concentration increased in the group of older men, regardless of their physical activity level. Along with this increase, there were no changes in nitro-oxidative stress or inflammation marker levels. MDPI 2021-07-16 /pmc/articles/PMC8301999/ /pubmed/34356664 http://dx.doi.org/10.3390/biom11071041 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wiecek, Magdalena
Szygula, Zbigniew
Gradek, Joanna
Kusmierczyk, Justyna
Szymura, Jadwiga
Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men
title Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men
title_full Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men
title_fullStr Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men
title_full_unstemmed Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men
title_short Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men
title_sort whole-body cryotherapy increases the activity of nitric oxide synthase in older men
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301999/
https://www.ncbi.nlm.nih.gov/pubmed/34356664
http://dx.doi.org/10.3390/biom11071041
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