Cargando…

Measuring the absolute abundance of the microbiome by adding yeast containing 16S rRNA gene from a hyperthermophile

High‐throughput sequencing (HTS) of 16S rRNA gene amplicons provides compositional information regarding the microbial community, but not the absolute abundance of the bacteria. We aimed to develop a standardized method for quantifying the absolute abundance of bacteria in microbiome studies. To dem...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kim, Ju Yeong, Yi, Myung‐hee, Kim, Myungjun, Lee, Seogwon, Moon, Hye Su, Yong, Dongeun, Yong, Tai‐Soon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302012/ https://www.ncbi.nlm.nih.gov/pubmed/34459541 http://dx.doi.org/10.1002/mbo3.1220

Ejemplares similares

Microbiome of Haemaphysalis longicornis Tick in Korea
por: Kim, Myungjun, et al.
Publicado: (2021)

Comparative Microbiome Analysis of Three Species of Laboratory-Reared Periplaneta Cockroaches
por: Lee, Seogwon, et al.
Publicado: (2020)

Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin
por: Kim, Ju Yeong, et al.
Publicado: (2023)

Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment
por: Lee, Seogwon, et al.
Publicado: (2021)

Investigating the microbiome of house dust mites in South Korea
por: Yi, Myung-hee, et al.
Publicado: (2023)

Comparative microbiomes of ticks collected from a black rhino and its surrounding environment
por: Lee, Seogwon, et al.
Publicado: (2019)

Ampicillin treated German cockroach extract leads to reduced inflammation in human lung cells and a mouse model of Asthma
por: Lee, Seogwon, et al.
Publicado: (2023)

Metabarcoding of bacteria and parasites in the gut of Apodemus agrarius
por: Kim, Soo Lim, et al.
Publicado: (2022)

Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System
por: Choi, Jun Ho, et al.
Publicado: (2021)

Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike‐in standard
por: Zemb, Olivier, et al.
Publicado: (2020)

Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome
por: Tettamanti Boshier, Florencia A., et al.
Publicado: (2020)

Diagnosis of Balamuthia mandrillaris Encephalitis by Thymine-Adenine Cloning Using Universal Eukaryotic Primers
por: Kim, Ju Yeong, et al.
Publicado: (2022)

Review of the Current State of Freely Accessible Web Tools for the Analysis of 16S rRNA Sequencing of the Gut Microbiome
por: Ibal, Jerald Conrad, et al.
Publicado: (2022)

Comprehensive 16S rRNA and metagenomic data from the gut microbiome of aging and rejuvenation mouse models
por: Shin, Jongoh, et al.
Publicado: (2022)

Bioconversion of CO to formate by artificially designed carbon monoxide:formate oxidoreductase in hyperthermophilic archaea
por: Lim, Jae Kyu, et al.
Publicado: (2022)

The Genboree Microbiome Toolset and the analysis of 16S rRNA microbial sequences
por: Riehle, Kevin, et al.
Publicado: (2012)

Exploring the Hospital Microbiome by High-Resolution 16S rRNA Profiling
por: Rampelotto, Pabulo H., et al.
Publicado: (2019)

Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing
por: Espiritu, Hector M., et al.
Publicado: (2020)

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility
por: Wang, Jian-Mei, et al.
Publicado: (2020)

Absolute Isotopic Abundance of Terrestrial Silver
por: Shields, William R., et al.
Publicado: (1962)

Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing
por: Shin, Jongoh, et al.
Publicado: (2016)

Oral Microbiome Profiles: 16S rRNA Pyrosequencing and Microarray Assay Comparison
por: Ahn, Jiyoung, et al.
Publicado: (2011)

Metaproteomic and 16S rRNA Gene Sequencing Analysis of the Infant Fecal Microbiome
por: Cortes, Laetitia, et al.
Publicado: (2019)

Gut Microbiome Dysbiosis in Patients with Endometrial Cancer vs. Healthy Controls Based on 16S rRNA Gene Sequencing
por: Li, Yue, et al.
Publicado: (2023)

MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons
por: Rozas, Miquel, et al.
Publicado: (2022)

The role of disulfide bond in hyperthermophilic endocellulase
por: Kim, Han-Woo, et al.
Publicado: (2013)

Population Abundance of Potentially Pathogenic Organisms in Intestinal Microbiome of Jungle Crow (Corvus macrorhynchos) Shown with 16S rRNA Gene-Based Microbial Community Analysis
por: Maeda, Isamu, et al.
Publicado: (2013)

Bypassing rRNA methylation by RsmA/Dim1during ribosome maturation in the hyperthermophilic archaeon Nanoarchaeum equitans
por: Seistrup, Kenneth H., et al.
Publicado: (2017)

A Core Human Microbiome as Viewed through 16S rRNA Sequence Clusters
por: Huse, Susan M., et al.
Publicado: (2012)

Intrinsic challenges in ancient microbiome reconstruction using 16S rRNA gene amplification
por: Ziesemer, Kirsten A., et al.
Publicado: (2015)

The impact of storage conditions on human stool 16S rRNA microbiome composition and diversity
por: Carruthers, Lauren V., et al.
Publicado: (2019)

Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
por: Johnson, Jethro S., et al.
Publicado: (2019)

Characterization of the Duodenal Mucosal Microbiome in Obese Adult Subjects by 16S rRNA Sequencing
por: Nardelli, Carmela, et al.
Publicado: (2020)

Comparative Analysis of 16S rRNA Gene and Metagenome Sequencing in Pediatric Gut Microbiomes
por: Peterson, Danielle, et al.
Publicado: (2021)

Absolute Quantification of Endogenous Ras Isoform Abundance
por: Mageean, Craig J., et al.
Publicado: (2015)

Absolute quantitation of microbiota abundance in environmental samples
por: Tkacz, Andrzej, et al.
Publicado: (2018)

Human reference gut microbiome catalog including newly assembled genomes from under-represented Asian metagenomes
por: Kim, Chan Yeong, et al.
Publicado: (2021)

Characterization of the Relative Abundance of the Citrus Pathogen Ca. Liberibacter Asiaticus in the Microbiome of Its Insect Vector, Diaphorina citri, using High Throughput 16S rRNA Sequencing
por: Fagen, Jennie R, et al.
Publicado: (2012)

Characterization of copy numbers of 16S rDNA and 16S rRNA of Candidatus Liberibacter asiaticus and the implication in detection in planta using quantitative PCR
por: Kim, Jeong-soon, et al.
Publicado: (2009)

Corrigendum: Intrinsic challenges in ancient microbiome reconstruction using 16S rRNA gene amplification
por: Ziesemer, Kirsten A., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_d3dsg5jpci4p902tj31irt769c