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Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells

Human pluripotent stem cell (hPSC)-derived organoids and cells have similar characteristics to human organs and tissues. Thus, in vitro human organoids and cells serve as a superior alternative to conventional cell lines and animal models in drug development and regenerative medicine. For a simple a...

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Autores principales: Lee, Mi-Ok, Lee, Su-gi, Jung, Cho-Rok, Son, Ye Seul, Ryu, Jae-Woon, Jung, Kwang Bo, Ahn, Jun-Ho, Oh, Jung-Hwa, Lee, Hyang-Ae, Lim, Jung Hwa, Kim, Janghwan, Jang, Insu, Choi, Jinhyuk, Jung, Jaeeun, Park, Kunhyang, Lee, Byungwook, Kim, Dae-Soo, Son, Mi-Young, Cho, Hyun-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302568/
https://www.ncbi.nlm.nih.gov/pubmed/34301945
http://dx.doi.org/10.1038/s41467-021-24746-w
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author Lee, Mi-Ok
Lee, Su-gi
Jung, Cho-Rok
Son, Ye Seul
Ryu, Jae-Woon
Jung, Kwang Bo
Ahn, Jun-Ho
Oh, Jung-Hwa
Lee, Hyang-Ae
Lim, Jung Hwa
Kim, Janghwan
Jang, Insu
Choi, Jinhyuk
Jung, Jaeeun
Park, Kunhyang
Lee, Byungwook
Kim, Dae-Soo
Son, Mi-Young
Cho, Hyun-Soo
author_facet Lee, Mi-Ok
Lee, Su-gi
Jung, Cho-Rok
Son, Ye Seul
Ryu, Jae-Woon
Jung, Kwang Bo
Ahn, Jun-Ho
Oh, Jung-Hwa
Lee, Hyang-Ae
Lim, Jung Hwa
Kim, Janghwan
Jang, Insu
Choi, Jinhyuk
Jung, Jaeeun
Park, Kunhyang
Lee, Byungwook
Kim, Dae-Soo
Son, Mi-Young
Cho, Hyun-Soo
author_sort Lee, Mi-Ok
collection PubMed
description Human pluripotent stem cell (hPSC)-derived organoids and cells have similar characteristics to human organs and tissues. Thus, in vitro human organoids and cells serve as a superior alternative to conventional cell lines and animal models in drug development and regenerative medicine. For a simple and reproducible analysis of the quality of organoids and cells to compensate for the shortcomings of existing experimental validation studies, a quantitative evaluation method should be developed. Here, using the GTEx database, we construct a quantitative calculation system to assess similarity to the human organs. To evaluate our system, we generate hPSC-derived organoids and cells, and detected organ similarity. To facilitate the access of our system by researchers, we develop a web-based user interface presenting similarity to the appropriate organs as percentages. Thus, this program could provide valuable information for the generation of high-quality organoids and cells and a strategy to guide proper lineage-oriented differentiation.
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spelling pubmed-83025682021-08-12 Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells Lee, Mi-Ok Lee, Su-gi Jung, Cho-Rok Son, Ye Seul Ryu, Jae-Woon Jung, Kwang Bo Ahn, Jun-Ho Oh, Jung-Hwa Lee, Hyang-Ae Lim, Jung Hwa Kim, Janghwan Jang, Insu Choi, Jinhyuk Jung, Jaeeun Park, Kunhyang Lee, Byungwook Kim, Dae-Soo Son, Mi-Young Cho, Hyun-Soo Nat Commun Article Human pluripotent stem cell (hPSC)-derived organoids and cells have similar characteristics to human organs and tissues. Thus, in vitro human organoids and cells serve as a superior alternative to conventional cell lines and animal models in drug development and regenerative medicine. For a simple and reproducible analysis of the quality of organoids and cells to compensate for the shortcomings of existing experimental validation studies, a quantitative evaluation method should be developed. Here, using the GTEx database, we construct a quantitative calculation system to assess similarity to the human organs. To evaluate our system, we generate hPSC-derived organoids and cells, and detected organ similarity. To facilitate the access of our system by researchers, we develop a web-based user interface presenting similarity to the appropriate organs as percentages. Thus, this program could provide valuable information for the generation of high-quality organoids and cells and a strategy to guide proper lineage-oriented differentiation. Nature Publishing Group UK 2021-07-23 /pmc/articles/PMC8302568/ /pubmed/34301945 http://dx.doi.org/10.1038/s41467-021-24746-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lee, Mi-Ok
Lee, Su-gi
Jung, Cho-Rok
Son, Ye Seul
Ryu, Jae-Woon
Jung, Kwang Bo
Ahn, Jun-Ho
Oh, Jung-Hwa
Lee, Hyang-Ae
Lim, Jung Hwa
Kim, Janghwan
Jang, Insu
Choi, Jinhyuk
Jung, Jaeeun
Park, Kunhyang
Lee, Byungwook
Kim, Dae-Soo
Son, Mi-Young
Cho, Hyun-Soo
Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells
title Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells
title_full Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells
title_fullStr Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells
title_full_unstemmed Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells
title_short Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells
title_sort development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302568/
https://www.ncbi.nlm.nih.gov/pubmed/34301945
http://dx.doi.org/10.1038/s41467-021-24746-w
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