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In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium

Ca-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (MAO). Subsequently, the MAO-coated zirconium surfaces were covered with an antimicrobial chitosan layer via the dip coating method to develop an antimicrobial, bioactive, and biocompatible composite biop...

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Autores principales: Aktug, Salim Levent, Durdu, Salih, Kalkan, Selin, Cavusoglu, Kultigin, Usta, Metin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302640/
https://www.ncbi.nlm.nih.gov/pubmed/34302008
http://dx.doi.org/10.1038/s41598-021-94502-z
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author Aktug, Salim Levent
Durdu, Salih
Kalkan, Selin
Cavusoglu, Kultigin
Usta, Metin
author_facet Aktug, Salim Levent
Durdu, Salih
Kalkan, Selin
Cavusoglu, Kultigin
Usta, Metin
author_sort Aktug, Salim Levent
collection PubMed
description Ca-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (MAO). Subsequently, the MAO-coated zirconium surfaces were covered with an antimicrobial chitosan layer via the dip coating method to develop an antimicrobial, bioactive, and biocompatible composite biopolymer and bioceramic layer for implant applications. Cubic ZrO(2), metastable Ca(0.15)Zr(0.85)O(1.85), and Ca(3)(PO(4))(2) were detected on the MAO surface by powder-XRD. The existence of chitosan on the MAO-coated Zr surfaces was verified by FTIR. The micropores and thermal cracks on the bioceramic MAO surface were sealed using a chitosan coating, where the MAO surface was porous and rough. All elements such as Zr, O, Ca, P, and C were homogenously distributed across both surfaces. Moreover, both surfaces indicated hydrophobic properties. However, the contact angle of the MAO surface was lower than that of the chitosan-based MAO surface. In vitro bioactivity on both surfaces was investigated via XRD, SEM, and EDX analyses post-immersion in simulated body fluid (SBF) for 14 days. In vitro bioactivity was significantly enhanced on the chitosan-based MAO surface with respect to the MAO surface. In vitro microbial adhesions on the chitosan-based MAO surfaces were lower than the MAO surfaces for Staphylococcus aureus and Escherichia coli.
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spelling pubmed-83026402021-07-27 In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium Aktug, Salim Levent Durdu, Salih Kalkan, Selin Cavusoglu, Kultigin Usta, Metin Sci Rep Article Ca-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (MAO). Subsequently, the MAO-coated zirconium surfaces were covered with an antimicrobial chitosan layer via the dip coating method to develop an antimicrobial, bioactive, and biocompatible composite biopolymer and bioceramic layer for implant applications. Cubic ZrO(2), metastable Ca(0.15)Zr(0.85)O(1.85), and Ca(3)(PO(4))(2) were detected on the MAO surface by powder-XRD. The existence of chitosan on the MAO-coated Zr surfaces was verified by FTIR. The micropores and thermal cracks on the bioceramic MAO surface were sealed using a chitosan coating, where the MAO surface was porous and rough. All elements such as Zr, O, Ca, P, and C were homogenously distributed across both surfaces. Moreover, both surfaces indicated hydrophobic properties. However, the contact angle of the MAO surface was lower than that of the chitosan-based MAO surface. In vitro bioactivity on both surfaces was investigated via XRD, SEM, and EDX analyses post-immersion in simulated body fluid (SBF) for 14 days. In vitro bioactivity was significantly enhanced on the chitosan-based MAO surface with respect to the MAO surface. In vitro microbial adhesions on the chitosan-based MAO surfaces were lower than the MAO surfaces for Staphylococcus aureus and Escherichia coli. Nature Publishing Group UK 2021-07-23 /pmc/articles/PMC8302640/ /pubmed/34302008 http://dx.doi.org/10.1038/s41598-021-94502-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Aktug, Salim Levent
Durdu, Salih
Kalkan, Selin
Cavusoglu, Kultigin
Usta, Metin
In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium
title In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium
title_full In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium
title_fullStr In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium
title_full_unstemmed In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium
title_short In vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium
title_sort in vitro biological and antimicrobial properties of chitosan-based bioceramic coatings on zirconium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302640/
https://www.ncbi.nlm.nih.gov/pubmed/34302008
http://dx.doi.org/10.1038/s41598-021-94502-z
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