Cargando…

Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic

Recently, as is evident with the COVID-19 pandemic, virus-containing aerosols can rapidly spread worldwide. As a consequence, filtering facepieces (FFP) are essential tools to protect against airborne viral particles. Incorrect donning and doffing of masks and a lack of hand-hygiene cause contagion...

Descripción completa

Detalles Bibliográficos
Autores principales: Tatzber, Franz, Wonisch, Willibald, Balka, Gyula, Marosi, Andras, Rusvai, Miklós, Resch, Ulrike, Lindschinger, Meinrad, Moerkl, Sabrina, Cvirn, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303125/
https://www.ncbi.nlm.nih.gov/pubmed/34299856
http://dx.doi.org/10.3390/ijerph18147406
_version_ 1783727011781410816
author Tatzber, Franz
Wonisch, Willibald
Balka, Gyula
Marosi, Andras
Rusvai, Miklós
Resch, Ulrike
Lindschinger, Meinrad
Moerkl, Sabrina
Cvirn, Gerhard
author_facet Tatzber, Franz
Wonisch, Willibald
Balka, Gyula
Marosi, Andras
Rusvai, Miklós
Resch, Ulrike
Lindschinger, Meinrad
Moerkl, Sabrina
Cvirn, Gerhard
author_sort Tatzber, Franz
collection PubMed
description Recently, as is evident with the COVID-19 pandemic, virus-containing aerosols can rapidly spread worldwide. As a consequence, filtering facepieces (FFP) are essential tools to protect against airborne viral particles. Incorrect donning and doffing of masks and a lack of hand-hygiene cause contagion by the wearers’ own hands. This study aimed to prove that hypertonic saline effectively reduces the infectious viral load on treated masks. Therefore, a hypertonic salt solution´s protective effect on surgical masks was investigated, specifically analyzing the infectivity of aerosolized Alphacoronavirus 1 in pigs (Transmissible Gastroenteritis Virus (TGEV)). Uncoated and hypertonic salt pre-coated FFPs were sprayed with TGEV. After drying, a defined part of the mask was rinsed with the medium, and the eluent was used for the infection of a porcine testicular cell line. Additionally, airborne microorganisms´ long-term infectivity of sodium-chloride in phosphate-buffered saline comprising 5% saccharose was investigated. In the results from an initial Median Tissue Culture Infectious Dose, infection rate of TGEV was minimally reduced by untreated FFP. In contrast, this could be reduced by a factor of 10(4) if FFPs were treated with hypertonic salt solutions. Airborne pathogens did not contaminate the growth medium if salt concentrations exceeded 5%. We conclude that hypertonic saline is a vital tool for anti-virus protection, exponentially improving the impact of FFPs.
format Online
Article
Text
id pubmed-8303125
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83031252021-07-25 Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic Tatzber, Franz Wonisch, Willibald Balka, Gyula Marosi, Andras Rusvai, Miklós Resch, Ulrike Lindschinger, Meinrad Moerkl, Sabrina Cvirn, Gerhard Int J Environ Res Public Health Brief Report Recently, as is evident with the COVID-19 pandemic, virus-containing aerosols can rapidly spread worldwide. As a consequence, filtering facepieces (FFP) are essential tools to protect against airborne viral particles. Incorrect donning and doffing of masks and a lack of hand-hygiene cause contagion by the wearers’ own hands. This study aimed to prove that hypertonic saline effectively reduces the infectious viral load on treated masks. Therefore, a hypertonic salt solution´s protective effect on surgical masks was investigated, specifically analyzing the infectivity of aerosolized Alphacoronavirus 1 in pigs (Transmissible Gastroenteritis Virus (TGEV)). Uncoated and hypertonic salt pre-coated FFPs were sprayed with TGEV. After drying, a defined part of the mask was rinsed with the medium, and the eluent was used for the infection of a porcine testicular cell line. Additionally, airborne microorganisms´ long-term infectivity of sodium-chloride in phosphate-buffered saline comprising 5% saccharose was investigated. In the results from an initial Median Tissue Culture Infectious Dose, infection rate of TGEV was minimally reduced by untreated FFP. In contrast, this could be reduced by a factor of 10(4) if FFPs were treated with hypertonic salt solutions. Airborne pathogens did not contaminate the growth medium if salt concentrations exceeded 5%. We conclude that hypertonic saline is a vital tool for anti-virus protection, exponentially improving the impact of FFPs. MDPI 2021-07-11 /pmc/articles/PMC8303125/ /pubmed/34299856 http://dx.doi.org/10.3390/ijerph18147406 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Tatzber, Franz
Wonisch, Willibald
Balka, Gyula
Marosi, Andras
Rusvai, Miklós
Resch, Ulrike
Lindschinger, Meinrad
Moerkl, Sabrina
Cvirn, Gerhard
Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic
title Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic
title_full Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic
title_fullStr Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic
title_full_unstemmed Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic
title_short Coating with Hypertonic Saline Improves Virus Protection of Filtering Facepiece Manyfold—Benefit of Salt Impregnation in Times of Pandemic
title_sort coating with hypertonic saline improves virus protection of filtering facepiece manyfold—benefit of salt impregnation in times of pandemic
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303125/
https://www.ncbi.nlm.nih.gov/pubmed/34299856
http://dx.doi.org/10.3390/ijerph18147406
work_keys_str_mv AT tatzberfranz coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic
AT wonischwillibald coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic
AT balkagyula coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic
AT marosiandras coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic
AT rusvaimiklos coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic
AT reschulrike coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic
AT lindschingermeinrad coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic
AT moerklsabrina coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic
AT cvirngerhard coatingwithhypertonicsalineimprovesvirusprotectionoffilteringfacepiecemanyfoldbenefitofsaltimpregnationintimesofpandemic