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Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions

Glucose is the primary fuel for the brain; its metabolism is linked with cerebral function. Different magnetic resonance spectroscopy (MRS) techniques are available to assess glucose metabolism, providing complementary information. Our first aim was to investigate the difference between hyperpolariz...

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Autores principales: Flatt, Emmanuelle, Lanz, Bernard, Pilloud, Yves, Capozzi, Andrea, Lerche, Mathilde Hauge, Gruetter, Rolf, Mishkovsky, Mor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303162/
https://www.ncbi.nlm.nih.gov/pubmed/34201777
http://dx.doi.org/10.3390/metabo11070413
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author Flatt, Emmanuelle
Lanz, Bernard
Pilloud, Yves
Capozzi, Andrea
Lerche, Mathilde Hauge
Gruetter, Rolf
Mishkovsky, Mor
author_facet Flatt, Emmanuelle
Lanz, Bernard
Pilloud, Yves
Capozzi, Andrea
Lerche, Mathilde Hauge
Gruetter, Rolf
Mishkovsky, Mor
author_sort Flatt, Emmanuelle
collection PubMed
description Glucose is the primary fuel for the brain; its metabolism is linked with cerebral function. Different magnetic resonance spectroscopy (MRS) techniques are available to assess glucose metabolism, providing complementary information. Our first aim was to investigate the difference between hyperpolarized (13)C-glucose MRS and non-hyperpolarized (2)H-glucose MRS to interrogate cerebral glycolysis. Isoflurane anesthesia is commonly employed in preclinical MRS, but it affects cerebral hemodynamics and functional connectivity. A combination of low doses of isoflurane and medetomidine is routinely used in rodent functional magnetic resonance imaging (fMRI) and shows similar functional connectivity, as in awake animals. As glucose metabolism is tightly linked to neuronal activity, our second aim was to assess the impact of these two anesthetic conditions on the cerebral metabolism of glucose. Brain metabolism of hyperpolarized (13)C-glucose and non-hyperpolaized (2)H-glucose was monitored in two groups of mice in a 9.4 T MRI system. We found that the very different duration and temporal resolution of the two techniques enable highlighting the different aspects in glucose metabolism. We demonstrate (by numerical simulations) that hyperpolarized (13)C-glucose reports on de novo lactate synthesis and is sensitive to cerebral metabolic rate of glucose (CMR(Glc)). We show that variations in cerebral glucose metabolism, under different anesthesia, are reflected differently in hyperpolarized and non-hyperpolarized X-nuclei glucose MRS.
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spelling pubmed-83031622021-07-25 Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions Flatt, Emmanuelle Lanz, Bernard Pilloud, Yves Capozzi, Andrea Lerche, Mathilde Hauge Gruetter, Rolf Mishkovsky, Mor Metabolites Article Glucose is the primary fuel for the brain; its metabolism is linked with cerebral function. Different magnetic resonance spectroscopy (MRS) techniques are available to assess glucose metabolism, providing complementary information. Our first aim was to investigate the difference between hyperpolarized (13)C-glucose MRS and non-hyperpolarized (2)H-glucose MRS to interrogate cerebral glycolysis. Isoflurane anesthesia is commonly employed in preclinical MRS, but it affects cerebral hemodynamics and functional connectivity. A combination of low doses of isoflurane and medetomidine is routinely used in rodent functional magnetic resonance imaging (fMRI) and shows similar functional connectivity, as in awake animals. As glucose metabolism is tightly linked to neuronal activity, our second aim was to assess the impact of these two anesthetic conditions on the cerebral metabolism of glucose. Brain metabolism of hyperpolarized (13)C-glucose and non-hyperpolaized (2)H-glucose was monitored in two groups of mice in a 9.4 T MRI system. We found that the very different duration and temporal resolution of the two techniques enable highlighting the different aspects in glucose metabolism. We demonstrate (by numerical simulations) that hyperpolarized (13)C-glucose reports on de novo lactate synthesis and is sensitive to cerebral metabolic rate of glucose (CMR(Glc)). We show that variations in cerebral glucose metabolism, under different anesthesia, are reflected differently in hyperpolarized and non-hyperpolarized X-nuclei glucose MRS. MDPI 2021-06-23 /pmc/articles/PMC8303162/ /pubmed/34201777 http://dx.doi.org/10.3390/metabo11070413 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Flatt, Emmanuelle
Lanz, Bernard
Pilloud, Yves
Capozzi, Andrea
Lerche, Mathilde Hauge
Gruetter, Rolf
Mishkovsky, Mor
Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
title Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
title_full Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
title_fullStr Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
title_full_unstemmed Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
title_short Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
title_sort measuring glycolytic activity with hyperpolarized [(2)h(7), u-(13)c(6)] d-glucose in the naive mouse brain under different anesthetic conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303162/
https://www.ncbi.nlm.nih.gov/pubmed/34201777
http://dx.doi.org/10.3390/metabo11070413
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