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Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
Glucose is the primary fuel for the brain; its metabolism is linked with cerebral function. Different magnetic resonance spectroscopy (MRS) techniques are available to assess glucose metabolism, providing complementary information. Our first aim was to investigate the difference between hyperpolariz...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303162/ https://www.ncbi.nlm.nih.gov/pubmed/34201777 http://dx.doi.org/10.3390/metabo11070413 |
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author | Flatt, Emmanuelle Lanz, Bernard Pilloud, Yves Capozzi, Andrea Lerche, Mathilde Hauge Gruetter, Rolf Mishkovsky, Mor |
author_facet | Flatt, Emmanuelle Lanz, Bernard Pilloud, Yves Capozzi, Andrea Lerche, Mathilde Hauge Gruetter, Rolf Mishkovsky, Mor |
author_sort | Flatt, Emmanuelle |
collection | PubMed |
description | Glucose is the primary fuel for the brain; its metabolism is linked with cerebral function. Different magnetic resonance spectroscopy (MRS) techniques are available to assess glucose metabolism, providing complementary information. Our first aim was to investigate the difference between hyperpolarized (13)C-glucose MRS and non-hyperpolarized (2)H-glucose MRS to interrogate cerebral glycolysis. Isoflurane anesthesia is commonly employed in preclinical MRS, but it affects cerebral hemodynamics and functional connectivity. A combination of low doses of isoflurane and medetomidine is routinely used in rodent functional magnetic resonance imaging (fMRI) and shows similar functional connectivity, as in awake animals. As glucose metabolism is tightly linked to neuronal activity, our second aim was to assess the impact of these two anesthetic conditions on the cerebral metabolism of glucose. Brain metabolism of hyperpolarized (13)C-glucose and non-hyperpolaized (2)H-glucose was monitored in two groups of mice in a 9.4 T MRI system. We found that the very different duration and temporal resolution of the two techniques enable highlighting the different aspects in glucose metabolism. We demonstrate (by numerical simulations) that hyperpolarized (13)C-glucose reports on de novo lactate synthesis and is sensitive to cerebral metabolic rate of glucose (CMR(Glc)). We show that variations in cerebral glucose metabolism, under different anesthesia, are reflected differently in hyperpolarized and non-hyperpolarized X-nuclei glucose MRS. |
format | Online Article Text |
id | pubmed-8303162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83031622021-07-25 Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions Flatt, Emmanuelle Lanz, Bernard Pilloud, Yves Capozzi, Andrea Lerche, Mathilde Hauge Gruetter, Rolf Mishkovsky, Mor Metabolites Article Glucose is the primary fuel for the brain; its metabolism is linked with cerebral function. Different magnetic resonance spectroscopy (MRS) techniques are available to assess glucose metabolism, providing complementary information. Our first aim was to investigate the difference between hyperpolarized (13)C-glucose MRS and non-hyperpolarized (2)H-glucose MRS to interrogate cerebral glycolysis. Isoflurane anesthesia is commonly employed in preclinical MRS, but it affects cerebral hemodynamics and functional connectivity. A combination of low doses of isoflurane and medetomidine is routinely used in rodent functional magnetic resonance imaging (fMRI) and shows similar functional connectivity, as in awake animals. As glucose metabolism is tightly linked to neuronal activity, our second aim was to assess the impact of these two anesthetic conditions on the cerebral metabolism of glucose. Brain metabolism of hyperpolarized (13)C-glucose and non-hyperpolaized (2)H-glucose was monitored in two groups of mice in a 9.4 T MRI system. We found that the very different duration and temporal resolution of the two techniques enable highlighting the different aspects in glucose metabolism. We demonstrate (by numerical simulations) that hyperpolarized (13)C-glucose reports on de novo lactate synthesis and is sensitive to cerebral metabolic rate of glucose (CMR(Glc)). We show that variations in cerebral glucose metabolism, under different anesthesia, are reflected differently in hyperpolarized and non-hyperpolarized X-nuclei glucose MRS. MDPI 2021-06-23 /pmc/articles/PMC8303162/ /pubmed/34201777 http://dx.doi.org/10.3390/metabo11070413 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Flatt, Emmanuelle Lanz, Bernard Pilloud, Yves Capozzi, Andrea Lerche, Mathilde Hauge Gruetter, Rolf Mishkovsky, Mor Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions |
title | Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions |
title_full | Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions |
title_fullStr | Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions |
title_full_unstemmed | Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions |
title_short | Measuring Glycolytic Activity with Hyperpolarized [(2)H(7), U-(13)C(6)] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions |
title_sort | measuring glycolytic activity with hyperpolarized [(2)h(7), u-(13)c(6)] d-glucose in the naive mouse brain under different anesthetic conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303162/ https://www.ncbi.nlm.nih.gov/pubmed/34201777 http://dx.doi.org/10.3390/metabo11070413 |
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