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The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids

Nonviral vectors for gene therapy such as lipoplexes are characterized by low toxicity, high biocompatibility, and good transfection efficiency. Specifically, lipoplexes based on polymeric surfactants and phospholipids have great potential as gene carriers due to the increased ability to bind geneti...

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Autores principales: Polańska, Żaneta, Pietralik-Molińska, Zuzanna, Wojciechowska, Daria, Moliński, Augustyn, Weiss, Marek, Skrzypczak, Andrzej, Kozak, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303235/
https://www.ncbi.nlm.nih.gov/pubmed/34299360
http://dx.doi.org/10.3390/ijms22147744
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author Polańska, Żaneta
Pietralik-Molińska, Zuzanna
Wojciechowska, Daria
Moliński, Augustyn
Weiss, Marek
Skrzypczak, Andrzej
Kozak, Maciej
author_facet Polańska, Żaneta
Pietralik-Molińska, Zuzanna
Wojciechowska, Daria
Moliński, Augustyn
Weiss, Marek
Skrzypczak, Andrzej
Kozak, Maciej
author_sort Polańska, Żaneta
collection PubMed
description Nonviral vectors for gene therapy such as lipoplexes are characterized by low toxicity, high biocompatibility, and good transfection efficiency. Specifically, lipoplexes based on polymeric surfactants and phospholipids have great potential as gene carriers due to the increased ability to bind genetic material (multiplied positive electric charge) while lowering undesirable effects (the presence of lipids makes the system more like natural membranes). This study aimed to test the ability to bind and release genetic material by lipoplexes based on trimeric surfactants and lipid formulations of different compositions and to characterize formed complexes by circular dichroism (CD) spectroscopy and atomic force microscopy (AFM). The cytotoxicity of studied lipoplexes was tested on HeLa cells by the MTT cell viability assay and the dye exclusion test (trypan blue). The presence of lipids in the system lowered the surfactant concentration required for complexation (higher efficiency) and reduced the cytotoxicity of lipoplexes. Surfactant/lipids/DNA complexes were more stable than surfactant/DNA complexes. Surfactant molecules induced the genetic material condensation, but the presence of lipids significantly intensified this process. Systems based on trimeric surfactants and lipid formulations, particularly TRI_N and TRI_IMI systems, could be used as delivery carrier, and have proven to be highly effective, nontoxic, and universal for DNA of various lengths.
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spelling pubmed-83032352021-07-25 The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids Polańska, Żaneta Pietralik-Molińska, Zuzanna Wojciechowska, Daria Moliński, Augustyn Weiss, Marek Skrzypczak, Andrzej Kozak, Maciej Int J Mol Sci Article Nonviral vectors for gene therapy such as lipoplexes are characterized by low toxicity, high biocompatibility, and good transfection efficiency. Specifically, lipoplexes based on polymeric surfactants and phospholipids have great potential as gene carriers due to the increased ability to bind genetic material (multiplied positive electric charge) while lowering undesirable effects (the presence of lipids makes the system more like natural membranes). This study aimed to test the ability to bind and release genetic material by lipoplexes based on trimeric surfactants and lipid formulations of different compositions and to characterize formed complexes by circular dichroism (CD) spectroscopy and atomic force microscopy (AFM). The cytotoxicity of studied lipoplexes was tested on HeLa cells by the MTT cell viability assay and the dye exclusion test (trypan blue). The presence of lipids in the system lowered the surfactant concentration required for complexation (higher efficiency) and reduced the cytotoxicity of lipoplexes. Surfactant/lipids/DNA complexes were more stable than surfactant/DNA complexes. Surfactant molecules induced the genetic material condensation, but the presence of lipids significantly intensified this process. Systems based on trimeric surfactants and lipid formulations, particularly TRI_N and TRI_IMI systems, could be used as delivery carrier, and have proven to be highly effective, nontoxic, and universal for DNA of various lengths. MDPI 2021-07-20 /pmc/articles/PMC8303235/ /pubmed/34299360 http://dx.doi.org/10.3390/ijms22147744 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Polańska, Żaneta
Pietralik-Molińska, Zuzanna
Wojciechowska, Daria
Moliński, Augustyn
Weiss, Marek
Skrzypczak, Andrzej
Kozak, Maciej
The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids
title The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids
title_full The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids
title_fullStr The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids
title_full_unstemmed The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids
title_short The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids
title_sort process of binding and releasing of genetic material from lipoplexes based on trimeric surfactants and phospholipids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303235/
https://www.ncbi.nlm.nih.gov/pubmed/34299360
http://dx.doi.org/10.3390/ijms22147744
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