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Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings
Brassinosteroids (BRs) are known to be essential regulators for wood formation in herbaceous plants and poplar, but their roles in secondary growth and xylem development are still not well-defined, especially in pines. Here, we treated Pinus massoniana seedlings with different concentrations of exog...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303313/ https://www.ncbi.nlm.nih.gov/pubmed/34299234 http://dx.doi.org/10.3390/ijms22147615 |
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author | Fan, Fuhua Zhou, Zijing Qin, Huijuan Tan, Jianhui Ding, Guijie |
author_facet | Fan, Fuhua Zhou, Zijing Qin, Huijuan Tan, Jianhui Ding, Guijie |
author_sort | Fan, Fuhua |
collection | PubMed |
description | Brassinosteroids (BRs) are known to be essential regulators for wood formation in herbaceous plants and poplar, but their roles in secondary growth and xylem development are still not well-defined, especially in pines. Here, we treated Pinus massoniana seedlings with different concentrations of exogenous BRs, and assayed the effects on plant growth, xylem development, endogenous phytohormone contents and gene expression within stems. Application of exogenous BR resulted in improving development of xylem more than phloem, and promoting xylem development in a dosage-dependent manner in a certain concentration rage. Endogenous hormone determination showed that BR may interact with other phytohormones in regulating xylem development. RNA-seq analysis revealed that some conventional phenylpropanoid biosynthesis- or lignin synthesis-related genes were downregulated, but the lignin content was elevated, suggesting that new lignin synthesis pathways or other cell wall components should be activated by BR treatment in P. massoniana. The results presented here reveal the foundational role of BRs in regulating plant secondary growth, and provide the basis for understanding molecular mechanisms of xylem development in P. massoniana. |
format | Online Article Text |
id | pubmed-8303313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83033132021-07-25 Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings Fan, Fuhua Zhou, Zijing Qin, Huijuan Tan, Jianhui Ding, Guijie Int J Mol Sci Article Brassinosteroids (BRs) are known to be essential regulators for wood formation in herbaceous plants and poplar, but their roles in secondary growth and xylem development are still not well-defined, especially in pines. Here, we treated Pinus massoniana seedlings with different concentrations of exogenous BRs, and assayed the effects on plant growth, xylem development, endogenous phytohormone contents and gene expression within stems. Application of exogenous BR resulted in improving development of xylem more than phloem, and promoting xylem development in a dosage-dependent manner in a certain concentration rage. Endogenous hormone determination showed that BR may interact with other phytohormones in regulating xylem development. RNA-seq analysis revealed that some conventional phenylpropanoid biosynthesis- or lignin synthesis-related genes were downregulated, but the lignin content was elevated, suggesting that new lignin synthesis pathways or other cell wall components should be activated by BR treatment in P. massoniana. The results presented here reveal the foundational role of BRs in regulating plant secondary growth, and provide the basis for understanding molecular mechanisms of xylem development in P. massoniana. MDPI 2021-07-16 /pmc/articles/PMC8303313/ /pubmed/34299234 http://dx.doi.org/10.3390/ijms22147615 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fan, Fuhua Zhou, Zijing Qin, Huijuan Tan, Jianhui Ding, Guijie Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings |
title | Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings |
title_full | Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings |
title_fullStr | Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings |
title_full_unstemmed | Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings |
title_short | Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings |
title_sort | exogenous brassinosteroid facilitates xylem development in pinus massoniana seedlings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303313/ https://www.ncbi.nlm.nih.gov/pubmed/34299234 http://dx.doi.org/10.3390/ijms22147615 |
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