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Preventing Extinction of the Critically Endangered Dactylorhiza incarnata subsp. ochroleuca in Britain Using Symbiotic Seedlings for Reintroduction

The yellow early marsh-orchid (Dactylorhiza incarnata subsp. ochroleuca) is critically endangered in the UK. Reintroduction of this threatened orchid to former haunts that have been restored is a long-term objective of this study. Identifying germination-specific mycorrhizal fungus lineages from clo...

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Autores principales: Sarasan, Viswambharan, Pankhurst, Tim, Yokoya, Kazutomo, Sriskandarajah, Sridevy, McDiarmid, Faye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303340/
https://www.ncbi.nlm.nih.gov/pubmed/34209355
http://dx.doi.org/10.3390/microorganisms9071421
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author Sarasan, Viswambharan
Pankhurst, Tim
Yokoya, Kazutomo
Sriskandarajah, Sridevy
McDiarmid, Faye
author_facet Sarasan, Viswambharan
Pankhurst, Tim
Yokoya, Kazutomo
Sriskandarajah, Sridevy
McDiarmid, Faye
author_sort Sarasan, Viswambharan
collection PubMed
description The yellow early marsh-orchid (Dactylorhiza incarnata subsp. ochroleuca) is critically endangered in the UK. Reintroduction of this threatened orchid to former haunts that have been restored is a long-term objective of this study. Identifying germination-specific mycorrhizal fungus lineages from closely related species is used as a method due to the extremely small number of plants left in the wild. A putative orchid mycorrhizal fungus of the family Tulasnellaceae, isolated from Dactylorhiza praetermissa, supported in vitro seed germination to produce reintroduction-ready seedlings. Reintroduced symbiotic seedlings survived over the winter months in the flooded reintroduction site (RS). The comparative soil analysis for key nutrients before reintroduction showed that phosphorus content in the RS is very low compared to the soil collected from the wild site (WS) where the last viable population exists. On the other hand, C:N ratio in the soil at the WS and RS were not significantly different. To our knowledge, this is the first-ever report on the reintroduction of symbiotic seedlings of a threatened orchid back to the wild in the UK.
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spelling pubmed-83033402021-07-25 Preventing Extinction of the Critically Endangered Dactylorhiza incarnata subsp. ochroleuca in Britain Using Symbiotic Seedlings for Reintroduction Sarasan, Viswambharan Pankhurst, Tim Yokoya, Kazutomo Sriskandarajah, Sridevy McDiarmid, Faye Microorganisms Article The yellow early marsh-orchid (Dactylorhiza incarnata subsp. ochroleuca) is critically endangered in the UK. Reintroduction of this threatened orchid to former haunts that have been restored is a long-term objective of this study. Identifying germination-specific mycorrhizal fungus lineages from closely related species is used as a method due to the extremely small number of plants left in the wild. A putative orchid mycorrhizal fungus of the family Tulasnellaceae, isolated from Dactylorhiza praetermissa, supported in vitro seed germination to produce reintroduction-ready seedlings. Reintroduced symbiotic seedlings survived over the winter months in the flooded reintroduction site (RS). The comparative soil analysis for key nutrients before reintroduction showed that phosphorus content in the RS is very low compared to the soil collected from the wild site (WS) where the last viable population exists. On the other hand, C:N ratio in the soil at the WS and RS were not significantly different. To our knowledge, this is the first-ever report on the reintroduction of symbiotic seedlings of a threatened orchid back to the wild in the UK. MDPI 2021-06-30 /pmc/articles/PMC8303340/ /pubmed/34209355 http://dx.doi.org/10.3390/microorganisms9071421 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sarasan, Viswambharan
Pankhurst, Tim
Yokoya, Kazutomo
Sriskandarajah, Sridevy
McDiarmid, Faye
Preventing Extinction of the Critically Endangered Dactylorhiza incarnata subsp. ochroleuca in Britain Using Symbiotic Seedlings for Reintroduction
title Preventing Extinction of the Critically Endangered Dactylorhiza incarnata subsp. ochroleuca in Britain Using Symbiotic Seedlings for Reintroduction
title_full Preventing Extinction of the Critically Endangered Dactylorhiza incarnata subsp. ochroleuca in Britain Using Symbiotic Seedlings for Reintroduction
title_fullStr Preventing Extinction of the Critically Endangered Dactylorhiza incarnata subsp. ochroleuca in Britain Using Symbiotic Seedlings for Reintroduction
title_full_unstemmed Preventing Extinction of the Critically Endangered Dactylorhiza incarnata subsp. ochroleuca in Britain Using Symbiotic Seedlings for Reintroduction
title_short Preventing Extinction of the Critically Endangered Dactylorhiza incarnata subsp. ochroleuca in Britain Using Symbiotic Seedlings for Reintroduction
title_sort preventing extinction of the critically endangered dactylorhiza incarnata subsp. ochroleuca in britain using symbiotic seedlings for reintroduction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303340/
https://www.ncbi.nlm.nih.gov/pubmed/34209355
http://dx.doi.org/10.3390/microorganisms9071421
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