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Estimating Real-Time qPCR Amplification Efficiency from Single-Reaction Data
Methods for estimating the qPCR amplification efficiency E from data for single reactions are tested on six multireplicate datasets, with emphasis on their performance as a function of the range of cycles n(1)–n(2) included in the analysis. The two-parameter exponential growth (EG) model that has be...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MDPI
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303528/ https://www.ncbi.nlm.nih.gov/pubmed/34357065 http://dx.doi.org/10.3390/life11070693 |