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Is NRXN1 Gene Expression an Important Marker of Treatment of Depressive Disorders? A Pilot Study

Aim: Due to the fact that NRXN1 is associated with neurodevelopmental disorders, the aim of this study was to investigate the role of the NRXN1 gene in the etiology and epigenetics of depression by comparison of NRXN1 mRNA expression and NRXN1 protein level expression in patients suffering from depr...

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Autores principales: Skiba, Aleksandra, Talarowska, Monika, Szemraj, Janusz, Gałecki, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303668/
https://www.ncbi.nlm.nih.gov/pubmed/34357104
http://dx.doi.org/10.3390/jpm11070637
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author Skiba, Aleksandra
Talarowska, Monika
Szemraj, Janusz
Gałecki, Piotr
author_facet Skiba, Aleksandra
Talarowska, Monika
Szemraj, Janusz
Gałecki, Piotr
author_sort Skiba, Aleksandra
collection PubMed
description Aim: Due to the fact that NRXN1 is associated with neurodevelopmental disorders, the aim of this study was to investigate the role of the NRXN1 gene in the etiology and epigenetics of depression by comparison of NRXN1 mRNA expression and NRXN1 protein level expression in patients suffering from depression versus healthy controls, as well as to search for clinical variables related to expression of the analyzed gene. Material and Methods: A total of 180 people aged 19–64 qualified for the study. The experimental group consisted of 97 people who were psychiatrically hospitalized, diagnosed with recurrent depressive disorders (F33) or who met the diagnostic criteria of a depressive episode (F32) according to ICD-10. The control group included 83 healthy people who volunteered to participate in the study. A sample of peripheral blood was obtained from people who were positively qualified to participate in the study—twice in the experimental group and once in the control group for genetic testing. Sociodemographic variables and data on the course of the disorder were also gathered. Patients were examined on study entry and at the end of the hospitalization with the Hamilton Depression Scale. Obtained data were analyzed statistically. The study was approved by the University’s Bioethics Committee. Results: The gene expression of NRXN1 at both mRNA and protein level significantly differs and it is lower in the experimental group compared to expression in healthy people. The difference in gene expression of NRXN1 at both the mRNA and protein levels between the first and second measurement in the experimental group is also significant. The result demonstrates a higher expression level in the first measurement and lower expression level in the second measurement when reported depression symptoms are less severe. Conclusions: Results concerning expression of NRXN1 may play an important role in further researches about the etiopathogenesis of depressive disorders such as looking for depression biomarkers and identifying evidence which may be relevant to personalize treatment for depression.
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spelling pubmed-83036682021-07-25 Is NRXN1 Gene Expression an Important Marker of Treatment of Depressive Disorders? A Pilot Study Skiba, Aleksandra Talarowska, Monika Szemraj, Janusz Gałecki, Piotr J Pers Med Article Aim: Due to the fact that NRXN1 is associated with neurodevelopmental disorders, the aim of this study was to investigate the role of the NRXN1 gene in the etiology and epigenetics of depression by comparison of NRXN1 mRNA expression and NRXN1 protein level expression in patients suffering from depression versus healthy controls, as well as to search for clinical variables related to expression of the analyzed gene. Material and Methods: A total of 180 people aged 19–64 qualified for the study. The experimental group consisted of 97 people who were psychiatrically hospitalized, diagnosed with recurrent depressive disorders (F33) or who met the diagnostic criteria of a depressive episode (F32) according to ICD-10. The control group included 83 healthy people who volunteered to participate in the study. A sample of peripheral blood was obtained from people who were positively qualified to participate in the study—twice in the experimental group and once in the control group for genetic testing. Sociodemographic variables and data on the course of the disorder were also gathered. Patients were examined on study entry and at the end of the hospitalization with the Hamilton Depression Scale. Obtained data were analyzed statistically. The study was approved by the University’s Bioethics Committee. Results: The gene expression of NRXN1 at both mRNA and protein level significantly differs and it is lower in the experimental group compared to expression in healthy people. The difference in gene expression of NRXN1 at both the mRNA and protein levels between the first and second measurement in the experimental group is also significant. The result demonstrates a higher expression level in the first measurement and lower expression level in the second measurement when reported depression symptoms are less severe. Conclusions: Results concerning expression of NRXN1 may play an important role in further researches about the etiopathogenesis of depressive disorders such as looking for depression biomarkers and identifying evidence which may be relevant to personalize treatment for depression. MDPI 2021-07-06 /pmc/articles/PMC8303668/ /pubmed/34357104 http://dx.doi.org/10.3390/jpm11070637 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Skiba, Aleksandra
Talarowska, Monika
Szemraj, Janusz
Gałecki, Piotr
Is NRXN1 Gene Expression an Important Marker of Treatment of Depressive Disorders? A Pilot Study
title Is NRXN1 Gene Expression an Important Marker of Treatment of Depressive Disorders? A Pilot Study
title_full Is NRXN1 Gene Expression an Important Marker of Treatment of Depressive Disorders? A Pilot Study
title_fullStr Is NRXN1 Gene Expression an Important Marker of Treatment of Depressive Disorders? A Pilot Study
title_full_unstemmed Is NRXN1 Gene Expression an Important Marker of Treatment of Depressive Disorders? A Pilot Study
title_short Is NRXN1 Gene Expression an Important Marker of Treatment of Depressive Disorders? A Pilot Study
title_sort is nrxn1 gene expression an important marker of treatment of depressive disorders? a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303668/
https://www.ncbi.nlm.nih.gov/pubmed/34357104
http://dx.doi.org/10.3390/jpm11070637
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