Cargando…

Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin

Endophytes associated with halophytes may contribute to the host’s adaptation to adverse environmental conditions through improving their stress tolerance and protecting them from various soil-borne pathogens. In this study, the diversity and antifungal activity of endophytic bacteria associated wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Lei, Ma, Jinbiao, Liu, Yonghong, Huang, Yin, Mohamad, Osama Abdalla Abdelshafy, Jiang, Hongchen, Egamberdieva, Dilfuza, Li, Wenjun, Li, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303770/
https://www.ncbi.nlm.nih.gov/pubmed/34361884
http://dx.doi.org/10.3390/microorganisms9071448
_version_ 1783727169221951488
author Gao, Lei
Ma, Jinbiao
Liu, Yonghong
Huang, Yin
Mohamad, Osama Abdalla Abdelshafy
Jiang, Hongchen
Egamberdieva, Dilfuza
Li, Wenjun
Li, Li
author_facet Gao, Lei
Ma, Jinbiao
Liu, Yonghong
Huang, Yin
Mohamad, Osama Abdalla Abdelshafy
Jiang, Hongchen
Egamberdieva, Dilfuza
Li, Wenjun
Li, Li
author_sort Gao, Lei
collection PubMed
description Endophytes associated with halophytes may contribute to the host’s adaptation to adverse environmental conditions through improving their stress tolerance and protecting them from various soil-borne pathogens. In this study, the diversity and antifungal activity of endophytic bacteria associated with halophytic samples growing on the shore of the western Aral Sea in Uzbekistan were investigated. The endophytic bacteria were isolated from the nine halophytic samples by using the culture-dependent method and identified according to their 16S rRNA gene sequences. The screening of endophytic bacterial isolates with the ability to inhibit pathogenic fungi was completed by the plate confrontation method. A total of 289 endophytic bacterial isolates were isolated from the nine halophytes, and they belong to Firmicutes, Actinobacteria, and Proteobacteria. The predominant genera of the isolated endophytic bacteria were Bacillus, Staphylococcus, and Streptomyces, accounting for 38.5%, 24.7%, and 12.5% of the total number of isolates, respectively. The comparative analysis indicated that the isolation effect was better for the sample S8, with the highest diversity and richness indices. The diversity index of the sample S7 was the lowest, while the richness index of samples S5 and S6 was the lowest. By comparing the isolation effect of 12 different media, it was found that the M7 medium had the best performance for isolating endophytic bacteria associated with halophytes in the western Aral Sea Basin. In addition, the results showed that only a few isolates have the ability to produce ex-enzymes, and eight and four endophytic bacterial isolates exhibited significant inhibition to the growth of Valsa mali and Verticillium dahlia, respectively. The results of this study indicated that halophytes are an important source for the selection of microbes that may protect plant from soil-borne pathogens.
format Online
Article
Text
id pubmed-8303770
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83037702021-07-25 Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin Gao, Lei Ma, Jinbiao Liu, Yonghong Huang, Yin Mohamad, Osama Abdalla Abdelshafy Jiang, Hongchen Egamberdieva, Dilfuza Li, Wenjun Li, Li Microorganisms Article Endophytes associated with halophytes may contribute to the host’s adaptation to adverse environmental conditions through improving their stress tolerance and protecting them from various soil-borne pathogens. In this study, the diversity and antifungal activity of endophytic bacteria associated with halophytic samples growing on the shore of the western Aral Sea in Uzbekistan were investigated. The endophytic bacteria were isolated from the nine halophytic samples by using the culture-dependent method and identified according to their 16S rRNA gene sequences. The screening of endophytic bacterial isolates with the ability to inhibit pathogenic fungi was completed by the plate confrontation method. A total of 289 endophytic bacterial isolates were isolated from the nine halophytes, and they belong to Firmicutes, Actinobacteria, and Proteobacteria. The predominant genera of the isolated endophytic bacteria were Bacillus, Staphylococcus, and Streptomyces, accounting for 38.5%, 24.7%, and 12.5% of the total number of isolates, respectively. The comparative analysis indicated that the isolation effect was better for the sample S8, with the highest diversity and richness indices. The diversity index of the sample S7 was the lowest, while the richness index of samples S5 and S6 was the lowest. By comparing the isolation effect of 12 different media, it was found that the M7 medium had the best performance for isolating endophytic bacteria associated with halophytes in the western Aral Sea Basin. In addition, the results showed that only a few isolates have the ability to produce ex-enzymes, and eight and four endophytic bacterial isolates exhibited significant inhibition to the growth of Valsa mali and Verticillium dahlia, respectively. The results of this study indicated that halophytes are an important source for the selection of microbes that may protect plant from soil-borne pathogens. MDPI 2021-07-06 /pmc/articles/PMC8303770/ /pubmed/34361884 http://dx.doi.org/10.3390/microorganisms9071448 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gao, Lei
Ma, Jinbiao
Liu, Yonghong
Huang, Yin
Mohamad, Osama Abdalla Abdelshafy
Jiang, Hongchen
Egamberdieva, Dilfuza
Li, Wenjun
Li, Li
Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin
title Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin
title_full Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin
title_fullStr Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin
title_full_unstemmed Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin
title_short Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin
title_sort diversity and biocontrol potential of cultivable endophytic bacteria associated with halophytes from the west aral sea basin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303770/
https://www.ncbi.nlm.nih.gov/pubmed/34361884
http://dx.doi.org/10.3390/microorganisms9071448
work_keys_str_mv AT gaolei diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin
AT majinbiao diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin
AT liuyonghong diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin
AT huangyin diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin
AT mohamadosamaabdallaabdelshafy diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin
AT jianghongchen diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin
AT egamberdievadilfuza diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin
AT liwenjun diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin
AT lili diversityandbiocontrolpotentialofcultivableendophyticbacteriaassociatedwithhalophytesfromthewestaralseabasin