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In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study

Bone injuries represent a major social and financial impairment, commonly requiring surgical intervention due to a limited healing capacity of the tissue, particularly regarding critical-sized defects and non-union fractures. Regenerative medicine with the application of bone implants has been devel...

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Autores principales: Branquinho, Mariana V., Ferreira, Sheila O., Alvites, Rui D., Magueta, Adriana F., Ivanov, Maxim, Sousa, Ana Catarina, Amorim, Irina, Faria, Fátima, Fernandes, M. H. V., Vilarinho, Paula M., Maurício, Ana Colette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303773/
https://www.ncbi.nlm.nih.gov/pubmed/34299274
http://dx.doi.org/10.3390/ijms22147655
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author Branquinho, Mariana V.
Ferreira, Sheila O.
Alvites, Rui D.
Magueta, Adriana F.
Ivanov, Maxim
Sousa, Ana Catarina
Amorim, Irina
Faria, Fátima
Fernandes, M. H. V.
Vilarinho, Paula M.
Maurício, Ana Colette
author_facet Branquinho, Mariana V.
Ferreira, Sheila O.
Alvites, Rui D.
Magueta, Adriana F.
Ivanov, Maxim
Sousa, Ana Catarina
Amorim, Irina
Faria, Fátima
Fernandes, M. H. V.
Vilarinho, Paula M.
Maurício, Ana Colette
author_sort Branquinho, Mariana V.
collection PubMed
description Bone injuries represent a major social and financial impairment, commonly requiring surgical intervention due to a limited healing capacity of the tissue, particularly regarding critical-sized defects and non-union fractures. Regenerative medicine with the application of bone implants has been developing in the past decades towards the manufacturing of appropriate devices. This work intended to evaluate medical 316L stainless steel (SS)-based devices covered by a polymer poly (L-lactic acid) (PLLA) coating for bone lesion mechanical and functional support. SS316L devices were subjected to a previously described silanization process, following a three-layer PLLA film coating. Devices were further characterized and evaluated towards their cytocompatibility and osteogenic potential using human dental pulp stem cells, and biocompatibility via subcutaneous implantation in a rat animal model. Results demonstrated PLLA-SS316L devices to present superior in vitro and in vivo outcomes and suggested the PLLA coating to provide osteo-inductive properties to the device. Overall, this work represents a preliminary study on PLLA-SS316L devices’ potential towards bone tissue regenerative techniques, showing promising outcomes for bone lesion support.
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spelling pubmed-83037732021-07-25 In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study Branquinho, Mariana V. Ferreira, Sheila O. Alvites, Rui D. Magueta, Adriana F. Ivanov, Maxim Sousa, Ana Catarina Amorim, Irina Faria, Fátima Fernandes, M. H. V. Vilarinho, Paula M. Maurício, Ana Colette Int J Mol Sci Article Bone injuries represent a major social and financial impairment, commonly requiring surgical intervention due to a limited healing capacity of the tissue, particularly regarding critical-sized defects and non-union fractures. Regenerative medicine with the application of bone implants has been developing in the past decades towards the manufacturing of appropriate devices. This work intended to evaluate medical 316L stainless steel (SS)-based devices covered by a polymer poly (L-lactic acid) (PLLA) coating for bone lesion mechanical and functional support. SS316L devices were subjected to a previously described silanization process, following a three-layer PLLA film coating. Devices were further characterized and evaluated towards their cytocompatibility and osteogenic potential using human dental pulp stem cells, and biocompatibility via subcutaneous implantation in a rat animal model. Results demonstrated PLLA-SS316L devices to present superior in vitro and in vivo outcomes and suggested the PLLA coating to provide osteo-inductive properties to the device. Overall, this work represents a preliminary study on PLLA-SS316L devices’ potential towards bone tissue regenerative techniques, showing promising outcomes for bone lesion support. MDPI 2021-07-17 /pmc/articles/PMC8303773/ /pubmed/34299274 http://dx.doi.org/10.3390/ijms22147655 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Branquinho, Mariana V.
Ferreira, Sheila O.
Alvites, Rui D.
Magueta, Adriana F.
Ivanov, Maxim
Sousa, Ana Catarina
Amorim, Irina
Faria, Fátima
Fernandes, M. H. V.
Vilarinho, Paula M.
Maurício, Ana Colette
In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study
title In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study
title_full In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study
title_fullStr In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study
title_full_unstemmed In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study
title_short In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study
title_sort in vitro and in vivo characterization of plla-316l stainless steel electromechanical devices for bone tissue engineering—a preliminary study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303773/
https://www.ncbi.nlm.nih.gov/pubmed/34299274
http://dx.doi.org/10.3390/ijms22147655
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