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In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study
Bone injuries represent a major social and financial impairment, commonly requiring surgical intervention due to a limited healing capacity of the tissue, particularly regarding critical-sized defects and non-union fractures. Regenerative medicine with the application of bone implants has been devel...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303773/ https://www.ncbi.nlm.nih.gov/pubmed/34299274 http://dx.doi.org/10.3390/ijms22147655 |
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author | Branquinho, Mariana V. Ferreira, Sheila O. Alvites, Rui D. Magueta, Adriana F. Ivanov, Maxim Sousa, Ana Catarina Amorim, Irina Faria, Fátima Fernandes, M. H. V. Vilarinho, Paula M. Maurício, Ana Colette |
author_facet | Branquinho, Mariana V. Ferreira, Sheila O. Alvites, Rui D. Magueta, Adriana F. Ivanov, Maxim Sousa, Ana Catarina Amorim, Irina Faria, Fátima Fernandes, M. H. V. Vilarinho, Paula M. Maurício, Ana Colette |
author_sort | Branquinho, Mariana V. |
collection | PubMed |
description | Bone injuries represent a major social and financial impairment, commonly requiring surgical intervention due to a limited healing capacity of the tissue, particularly regarding critical-sized defects and non-union fractures. Regenerative medicine with the application of bone implants has been developing in the past decades towards the manufacturing of appropriate devices. This work intended to evaluate medical 316L stainless steel (SS)-based devices covered by a polymer poly (L-lactic acid) (PLLA) coating for bone lesion mechanical and functional support. SS316L devices were subjected to a previously described silanization process, following a three-layer PLLA film coating. Devices were further characterized and evaluated towards their cytocompatibility and osteogenic potential using human dental pulp stem cells, and biocompatibility via subcutaneous implantation in a rat animal model. Results demonstrated PLLA-SS316L devices to present superior in vitro and in vivo outcomes and suggested the PLLA coating to provide osteo-inductive properties to the device. Overall, this work represents a preliminary study on PLLA-SS316L devices’ potential towards bone tissue regenerative techniques, showing promising outcomes for bone lesion support. |
format | Online Article Text |
id | pubmed-8303773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83037732021-07-25 In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study Branquinho, Mariana V. Ferreira, Sheila O. Alvites, Rui D. Magueta, Adriana F. Ivanov, Maxim Sousa, Ana Catarina Amorim, Irina Faria, Fátima Fernandes, M. H. V. Vilarinho, Paula M. Maurício, Ana Colette Int J Mol Sci Article Bone injuries represent a major social and financial impairment, commonly requiring surgical intervention due to a limited healing capacity of the tissue, particularly regarding critical-sized defects and non-union fractures. Regenerative medicine with the application of bone implants has been developing in the past decades towards the manufacturing of appropriate devices. This work intended to evaluate medical 316L stainless steel (SS)-based devices covered by a polymer poly (L-lactic acid) (PLLA) coating for bone lesion mechanical and functional support. SS316L devices were subjected to a previously described silanization process, following a three-layer PLLA film coating. Devices were further characterized and evaluated towards their cytocompatibility and osteogenic potential using human dental pulp stem cells, and biocompatibility via subcutaneous implantation in a rat animal model. Results demonstrated PLLA-SS316L devices to present superior in vitro and in vivo outcomes and suggested the PLLA coating to provide osteo-inductive properties to the device. Overall, this work represents a preliminary study on PLLA-SS316L devices’ potential towards bone tissue regenerative techniques, showing promising outcomes for bone lesion support. MDPI 2021-07-17 /pmc/articles/PMC8303773/ /pubmed/34299274 http://dx.doi.org/10.3390/ijms22147655 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Branquinho, Mariana V. Ferreira, Sheila O. Alvites, Rui D. Magueta, Adriana F. Ivanov, Maxim Sousa, Ana Catarina Amorim, Irina Faria, Fátima Fernandes, M. H. V. Vilarinho, Paula M. Maurício, Ana Colette In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study |
title | In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study |
title_full | In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study |
title_fullStr | In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study |
title_full_unstemmed | In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study |
title_short | In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering—A Preliminary Study |
title_sort | in vitro and in vivo characterization of plla-316l stainless steel electromechanical devices for bone tissue engineering—a preliminary study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303773/ https://www.ncbi.nlm.nih.gov/pubmed/34299274 http://dx.doi.org/10.3390/ijms22147655 |
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