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CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression
Circular RNAs (circRNAs), a class of covalently closed RNAs formed by a back-splicing reaction, have been involved in the regulation of diverse oncogenic processes. In this article we describe circVAMP3, a novel circular RNA overexpressed in RH4, a representative cell line of alveolar rhabdomyosarco...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303801/ https://www.ncbi.nlm.nih.gov/pubmed/34203273 http://dx.doi.org/10.3390/genes12070985 |
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author | Rossi, Francesca Centrón-Broco, Alvaro Dattilo, Dario Di Timoteo, Gaia Guarnacci, Marco Colantoni, Alessio Beltran Nebot, Manuel Bozzoni, Irene |
author_facet | Rossi, Francesca Centrón-Broco, Alvaro Dattilo, Dario Di Timoteo, Gaia Guarnacci, Marco Colantoni, Alessio Beltran Nebot, Manuel Bozzoni, Irene |
author_sort | Rossi, Francesca |
collection | PubMed |
description | Circular RNAs (circRNAs), a class of covalently closed RNAs formed by a back-splicing reaction, have been involved in the regulation of diverse oncogenic processes. In this article we describe circVAMP3, a novel circular RNA overexpressed in RH4, a representative cell line of alveolar rhabdomyosarcoma. We demonstrated that circVAMP3 has a differential m(6)A pattern opposed to its linear counterpart, suggesting that the two isoforms can be differently regulated by such RNA modification. Moreover, we show how circVAMP3 depletion in alveolar rhabdomyosarcoma cells can impair cell cycle progression, through the alteration of the AKT-related pathways, pointing to this non-coding RNA as a novel regulator of the alveolar rhabdomyosarcoma progression and as a putative future therapeutic target. |
format | Online Article Text |
id | pubmed-8303801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83038012021-07-25 CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression Rossi, Francesca Centrón-Broco, Alvaro Dattilo, Dario Di Timoteo, Gaia Guarnacci, Marco Colantoni, Alessio Beltran Nebot, Manuel Bozzoni, Irene Genes (Basel) Article Circular RNAs (circRNAs), a class of covalently closed RNAs formed by a back-splicing reaction, have been involved in the regulation of diverse oncogenic processes. In this article we describe circVAMP3, a novel circular RNA overexpressed in RH4, a representative cell line of alveolar rhabdomyosarcoma. We demonstrated that circVAMP3 has a differential m(6)A pattern opposed to its linear counterpart, suggesting that the two isoforms can be differently regulated by such RNA modification. Moreover, we show how circVAMP3 depletion in alveolar rhabdomyosarcoma cells can impair cell cycle progression, through the alteration of the AKT-related pathways, pointing to this non-coding RNA as a novel regulator of the alveolar rhabdomyosarcoma progression and as a putative future therapeutic target. MDPI 2021-06-28 /pmc/articles/PMC8303801/ /pubmed/34203273 http://dx.doi.org/10.3390/genes12070985 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rossi, Francesca Centrón-Broco, Alvaro Dattilo, Dario Di Timoteo, Gaia Guarnacci, Marco Colantoni, Alessio Beltran Nebot, Manuel Bozzoni, Irene CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression |
title | CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression |
title_full | CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression |
title_fullStr | CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression |
title_full_unstemmed | CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression |
title_short | CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression |
title_sort | circvamp3: a circrna with a role in alveolar rhabdomyosarcoma cell cycle progression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303801/ https://www.ncbi.nlm.nih.gov/pubmed/34203273 http://dx.doi.org/10.3390/genes12070985 |
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