Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan

Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to disti...

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Autores principales: Krylov, Vadim B., Solovev, Arsenii S., Puchkin, Ilya A., Yashunsky, Dmitry V., Antonets, Anna V., Kutsevalova, Olga Y., Nifantiev, Nikolay E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303853/
https://www.ncbi.nlm.nih.gov/pubmed/34202579
http://dx.doi.org/10.3390/jof7070504
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author Krylov, Vadim B.
Solovev, Arsenii S.
Puchkin, Ilya A.
Yashunsky, Dmitry V.
Antonets, Anna V.
Kutsevalova, Olga Y.
Nifantiev, Nikolay E.
author_facet Krylov, Vadim B.
Solovev, Arsenii S.
Puchkin, Ilya A.
Yashunsky, Dmitry V.
Antonets, Anna V.
Kutsevalova, Olga Y.
Nifantiev, Nikolay E.
author_sort Krylov, Vadim B.
collection PubMed
description Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.
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spelling pubmed-83038532021-07-25 Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan Krylov, Vadim B. Solovev, Arsenii S. Puchkin, Ilya A. Yashunsky, Dmitry V. Antonets, Anna V. Kutsevalova, Olga Y. Nifantiev, Nikolay E. J Fungi (Basel) Article Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously. MDPI 2021-06-24 /pmc/articles/PMC8303853/ /pubmed/34202579 http://dx.doi.org/10.3390/jof7070504 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krylov, Vadim B.
Solovev, Arsenii S.
Puchkin, Ilya A.
Yashunsky, Dmitry V.
Antonets, Anna V.
Kutsevalova, Olga Y.
Nifantiev, Nikolay E.
Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan
title Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan
title_full Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan
title_fullStr Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan
title_full_unstemmed Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan
title_short Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan
title_sort reinvestigation of carbohydrate specificity of ebca-1 monoclonal antibody used for the detection of candida mannan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303853/
https://www.ncbi.nlm.nih.gov/pubmed/34202579
http://dx.doi.org/10.3390/jof7070504
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