Cargando…

Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii

Microorganisms need to sense and adapt to fluctuations in the environmental pH. In fungal species, this response is mediated by the conserved pacC/RIM101 pathway. In Aspergillus nidulans, PacC activates alkaline-expressed genes and represses acid-controlled genes in response to alkaline pH and has i...

Descripción completa

Detalles Bibliográficos
Autores principales: Wasserstrom, Lisa, Wendland, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304098/
https://www.ncbi.nlm.nih.gov/pubmed/34209071
http://dx.doi.org/10.3390/jof7070527
_version_ 1783727251689308160
author Wasserstrom, Lisa
Wendland, Jürgen
author_facet Wasserstrom, Lisa
Wendland, Jürgen
author_sort Wasserstrom, Lisa
collection PubMed
description Microorganisms need to sense and adapt to fluctuations in the environmental pH. In fungal species, this response is mediated by the conserved pacC/RIM101 pathway. In Aspergillus nidulans, PacC activates alkaline-expressed genes and represses acid-controlled genes in response to alkaline pH and has important functions in regulating growth and conidia formation. In Saccharomyces cerevisiae, the PacC homolog Rim101 is required for adaptation to extracellular pH and to regulate transcription of IME1, the Initiator of MEiosis. S. cerevisiae rim101 mutants are defective in sporulation. In Ashbya gossypii, a filamentous fungus belonging to the family of Saccharomycetaceae, little is known about the role of pH in regulating growth and sporulation. Here, we deleted the AgRIM101 homolog (AFR190C). Our analyses show that Rim101 is important for growth and essential for sporulation at alkaline pH in A. gossypii. Acidic liquid sporulation media were alkalinized by sporulating strains, while the high pH of alkaline media (starting pH = 8.6) was reduced to a pH ~ 7.5 by these strains. However, Agrim101 mutants were unable to sporulate in alkaline media and failed to reduce the initial high pH, while they were capable of sporulation in acidic liquid media in which they increased the pH like the wild type.
format Online
Article
Text
id pubmed-8304098
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83040982021-07-25 Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii Wasserstrom, Lisa Wendland, Jürgen J Fungi (Basel) Communication Microorganisms need to sense and adapt to fluctuations in the environmental pH. In fungal species, this response is mediated by the conserved pacC/RIM101 pathway. In Aspergillus nidulans, PacC activates alkaline-expressed genes and represses acid-controlled genes in response to alkaline pH and has important functions in regulating growth and conidia formation. In Saccharomyces cerevisiae, the PacC homolog Rim101 is required for adaptation to extracellular pH and to regulate transcription of IME1, the Initiator of MEiosis. S. cerevisiae rim101 mutants are defective in sporulation. In Ashbya gossypii, a filamentous fungus belonging to the family of Saccharomycetaceae, little is known about the role of pH in regulating growth and sporulation. Here, we deleted the AgRIM101 homolog (AFR190C). Our analyses show that Rim101 is important for growth and essential for sporulation at alkaline pH in A. gossypii. Acidic liquid sporulation media were alkalinized by sporulating strains, while the high pH of alkaline media (starting pH = 8.6) was reduced to a pH ~ 7.5 by these strains. However, Agrim101 mutants were unable to sporulate in alkaline media and failed to reduce the initial high pH, while they were capable of sporulation in acidic liquid media in which they increased the pH like the wild type. MDPI 2021-06-30 /pmc/articles/PMC8304098/ /pubmed/34209071 http://dx.doi.org/10.3390/jof7070527 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Wasserstrom, Lisa
Wendland, Jürgen
Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii
title Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii
title_full Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii
title_fullStr Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii
title_full_unstemmed Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii
title_short Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii
title_sort role of rim101 for sporulation at alkaline ph in ashbya gossypii
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304098/
https://www.ncbi.nlm.nih.gov/pubmed/34209071
http://dx.doi.org/10.3390/jof7070527
work_keys_str_mv AT wasserstromlisa roleofrim101forsporulationatalkalinephinashbyagossypii
AT wendlandjurgen roleofrim101forsporulationatalkalinephinashbyagossypii