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Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii
Microorganisms need to sense and adapt to fluctuations in the environmental pH. In fungal species, this response is mediated by the conserved pacC/RIM101 pathway. In Aspergillus nidulans, PacC activates alkaline-expressed genes and represses acid-controlled genes in response to alkaline pH and has i...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304098/ https://www.ncbi.nlm.nih.gov/pubmed/34209071 http://dx.doi.org/10.3390/jof7070527 |
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author | Wasserstrom, Lisa Wendland, Jürgen |
author_facet | Wasserstrom, Lisa Wendland, Jürgen |
author_sort | Wasserstrom, Lisa |
collection | PubMed |
description | Microorganisms need to sense and adapt to fluctuations in the environmental pH. In fungal species, this response is mediated by the conserved pacC/RIM101 pathway. In Aspergillus nidulans, PacC activates alkaline-expressed genes and represses acid-controlled genes in response to alkaline pH and has important functions in regulating growth and conidia formation. In Saccharomyces cerevisiae, the PacC homolog Rim101 is required for adaptation to extracellular pH and to regulate transcription of IME1, the Initiator of MEiosis. S. cerevisiae rim101 mutants are defective in sporulation. In Ashbya gossypii, a filamentous fungus belonging to the family of Saccharomycetaceae, little is known about the role of pH in regulating growth and sporulation. Here, we deleted the AgRIM101 homolog (AFR190C). Our analyses show that Rim101 is important for growth and essential for sporulation at alkaline pH in A. gossypii. Acidic liquid sporulation media were alkalinized by sporulating strains, while the high pH of alkaline media (starting pH = 8.6) was reduced to a pH ~ 7.5 by these strains. However, Agrim101 mutants were unable to sporulate in alkaline media and failed to reduce the initial high pH, while they were capable of sporulation in acidic liquid media in which they increased the pH like the wild type. |
format | Online Article Text |
id | pubmed-8304098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83040982021-07-25 Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii Wasserstrom, Lisa Wendland, Jürgen J Fungi (Basel) Communication Microorganisms need to sense and adapt to fluctuations in the environmental pH. In fungal species, this response is mediated by the conserved pacC/RIM101 pathway. In Aspergillus nidulans, PacC activates alkaline-expressed genes and represses acid-controlled genes in response to alkaline pH and has important functions in regulating growth and conidia formation. In Saccharomyces cerevisiae, the PacC homolog Rim101 is required for adaptation to extracellular pH and to regulate transcription of IME1, the Initiator of MEiosis. S. cerevisiae rim101 mutants are defective in sporulation. In Ashbya gossypii, a filamentous fungus belonging to the family of Saccharomycetaceae, little is known about the role of pH in regulating growth and sporulation. Here, we deleted the AgRIM101 homolog (AFR190C). Our analyses show that Rim101 is important for growth and essential for sporulation at alkaline pH in A. gossypii. Acidic liquid sporulation media were alkalinized by sporulating strains, while the high pH of alkaline media (starting pH = 8.6) was reduced to a pH ~ 7.5 by these strains. However, Agrim101 mutants were unable to sporulate in alkaline media and failed to reduce the initial high pH, while they were capable of sporulation in acidic liquid media in which they increased the pH like the wild type. MDPI 2021-06-30 /pmc/articles/PMC8304098/ /pubmed/34209071 http://dx.doi.org/10.3390/jof7070527 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Wasserstrom, Lisa Wendland, Jürgen Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii |
title | Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii |
title_full | Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii |
title_fullStr | Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii |
title_full_unstemmed | Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii |
title_short | Role of RIM101 for Sporulation at Alkaline pH in Ashbya gossypii |
title_sort | role of rim101 for sporulation at alkaline ph in ashbya gossypii |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304098/ https://www.ncbi.nlm.nih.gov/pubmed/34209071 http://dx.doi.org/10.3390/jof7070527 |
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