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Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age

Although breast milk is considered the gold standard of nutrition for infant feeding, some circumstances may make breastfeeding difficult. Several commercial milk preparations include synthetic human milk oligosaccharides (HMOs) in their composition. However, the effect of HMOs on the establishment...

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Autores principales: Nogacka, Alicja M., Arboleya, Silvia, Nikpoor, Naghmeh, Auger, Jeremie, Salazar, Nuria, Cuesta, Isabel, Mantecón, Laura, Solís, Gonzalo, Gueimonde, Miguel, Tompkins, Thomas A., de los Reyes-Gavilán, Clara G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304384/
https://www.ncbi.nlm.nih.gov/pubmed/34361914
http://dx.doi.org/10.3390/microorganisms9071478
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author Nogacka, Alicja M.
Arboleya, Silvia
Nikpoor, Naghmeh
Auger, Jeremie
Salazar, Nuria
Cuesta, Isabel
Mantecón, Laura
Solís, Gonzalo
Gueimonde, Miguel
Tompkins, Thomas A.
de los Reyes-Gavilán, Clara G.
author_facet Nogacka, Alicja M.
Arboleya, Silvia
Nikpoor, Naghmeh
Auger, Jeremie
Salazar, Nuria
Cuesta, Isabel
Mantecón, Laura
Solís, Gonzalo
Gueimonde, Miguel
Tompkins, Thomas A.
de los Reyes-Gavilán, Clara G.
author_sort Nogacka, Alicja M.
collection PubMed
description Although breast milk is considered the gold standard of nutrition for infant feeding, some circumstances may make breastfeeding difficult. Several commercial milk preparations include synthetic human milk oligosaccharides (HMOs) in their composition. However, the effect of HMOs on the establishment of the intestinal microbiota remains incompletely understood. Independent batch fermentations were performed with feces from six full-term infant donors of two months of age (three breastfed and three formula-fed, exclusively) in the presence of 2′fucosyllactose (2′FL), one of the most abundant HMOs in human milk. Microbiota composition was analyzed by 16S rRNA gene sequencing at baseline and at 24 h of incubation. The 2′FL consumption, gas accumulation, and levels of different metabolites were determined by chromatography. Microbiota profiles at baseline were clearly influenced by the mode of feeding and by the intrinsic ability of microbiotas to degrade 2′FL. The 2′FL degradation rate clustered fecal cultures into slow and fast degraders, regardless of feeding type, this being a determinant factor influencing the evolution of the microbiota during incubation, although the low number of donors precludes drawing sound conclusions. More studies are needed to decipher the extent to which the early intervention with HMOs could influence the microbiota as a function of its ability to utilize 2′FL.
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spelling pubmed-83043842021-07-25 Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age Nogacka, Alicja M. Arboleya, Silvia Nikpoor, Naghmeh Auger, Jeremie Salazar, Nuria Cuesta, Isabel Mantecón, Laura Solís, Gonzalo Gueimonde, Miguel Tompkins, Thomas A. de los Reyes-Gavilán, Clara G. Microorganisms Article Although breast milk is considered the gold standard of nutrition for infant feeding, some circumstances may make breastfeeding difficult. Several commercial milk preparations include synthetic human milk oligosaccharides (HMOs) in their composition. However, the effect of HMOs on the establishment of the intestinal microbiota remains incompletely understood. Independent batch fermentations were performed with feces from six full-term infant donors of two months of age (three breastfed and three formula-fed, exclusively) in the presence of 2′fucosyllactose (2′FL), one of the most abundant HMOs in human milk. Microbiota composition was analyzed by 16S rRNA gene sequencing at baseline and at 24 h of incubation. The 2′FL consumption, gas accumulation, and levels of different metabolites were determined by chromatography. Microbiota profiles at baseline were clearly influenced by the mode of feeding and by the intrinsic ability of microbiotas to degrade 2′FL. The 2′FL degradation rate clustered fecal cultures into slow and fast degraders, regardless of feeding type, this being a determinant factor influencing the evolution of the microbiota during incubation, although the low number of donors precludes drawing sound conclusions. More studies are needed to decipher the extent to which the early intervention with HMOs could influence the microbiota as a function of its ability to utilize 2′FL. MDPI 2021-07-09 /pmc/articles/PMC8304384/ /pubmed/34361914 http://dx.doi.org/10.3390/microorganisms9071478 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nogacka, Alicja M.
Arboleya, Silvia
Nikpoor, Naghmeh
Auger, Jeremie
Salazar, Nuria
Cuesta, Isabel
Mantecón, Laura
Solís, Gonzalo
Gueimonde, Miguel
Tompkins, Thomas A.
de los Reyes-Gavilán, Clara G.
Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age
title Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age
title_full Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age
title_fullStr Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age
title_full_unstemmed Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age
title_short Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age
title_sort influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304384/
https://www.ncbi.nlm.nih.gov/pubmed/34361914
http://dx.doi.org/10.3390/microorganisms9071478
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