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Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography–Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells

Oxygen deficiency in cells, tissues, and organs can not only prevent the proper development of biological functions but it can also lead to several diseases and disorders. In this sense, the kidney deserves special attention since hypoxia can be considered an important factor in the pathophysiology...

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Autores principales: Bernardo-Bermejo, Samuel, Sánchez-López, Elena, Tan, Lei, Benito-Martínez, Selma, Jiang, Zhengjin, Castro-Puyana, María, Lucio-Cazaña, Francisco Javier, Marina, María Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304667/
https://www.ncbi.nlm.nih.gov/pubmed/34299017
http://dx.doi.org/10.3390/ijms22147399
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author Bernardo-Bermejo, Samuel
Sánchez-López, Elena
Tan, Lei
Benito-Martínez, Selma
Jiang, Zhengjin
Castro-Puyana, María
Lucio-Cazaña, Francisco Javier
Marina, María Luisa
author_facet Bernardo-Bermejo, Samuel
Sánchez-López, Elena
Tan, Lei
Benito-Martínez, Selma
Jiang, Zhengjin
Castro-Puyana, María
Lucio-Cazaña, Francisco Javier
Marina, María Luisa
author_sort Bernardo-Bermejo, Samuel
collection PubMed
description Oxygen deficiency in cells, tissues, and organs can not only prevent the proper development of biological functions but it can also lead to several diseases and disorders. In this sense, the kidney deserves special attention since hypoxia can be considered an important factor in the pathophysiology of both acute kidney injury and chronic kidney disease. To provide better knowledge to unveil the molecular mechanisms involved, new studies are necessary. In this sense, this work aims to study, for the first time, an in vitro model of hypoxia-induced metabolic alterations in human proximal tubular HK-2 cells because renal proximal tubules are particularly susceptible to hypoxia. Different groups of cells, cultivated under control and hypoxia conditions at 0.5, 5, 24, and 48 h, were investigated using untargeted metabolomic approaches based on reversed-phase liquid chromatography–mass spectrometry. Both intracellular and extracellular fluids were studied to obtain a large metabolite coverage. On the other hand, multivariate and univariate analyses were carried out to find the differences among the cell groups and to select the most relevant variables. The molecular features identified as affected metabolites were mainly amino acids and Amadori compounds. Insights about their biological relevance are also provided.
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spelling pubmed-83046672021-07-25 Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography–Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells Bernardo-Bermejo, Samuel Sánchez-López, Elena Tan, Lei Benito-Martínez, Selma Jiang, Zhengjin Castro-Puyana, María Lucio-Cazaña, Francisco Javier Marina, María Luisa Int J Mol Sci Article Oxygen deficiency in cells, tissues, and organs can not only prevent the proper development of biological functions but it can also lead to several diseases and disorders. In this sense, the kidney deserves special attention since hypoxia can be considered an important factor in the pathophysiology of both acute kidney injury and chronic kidney disease. To provide better knowledge to unveil the molecular mechanisms involved, new studies are necessary. In this sense, this work aims to study, for the first time, an in vitro model of hypoxia-induced metabolic alterations in human proximal tubular HK-2 cells because renal proximal tubules are particularly susceptible to hypoxia. Different groups of cells, cultivated under control and hypoxia conditions at 0.5, 5, 24, and 48 h, were investigated using untargeted metabolomic approaches based on reversed-phase liquid chromatography–mass spectrometry. Both intracellular and extracellular fluids were studied to obtain a large metabolite coverage. On the other hand, multivariate and univariate analyses were carried out to find the differences among the cell groups and to select the most relevant variables. The molecular features identified as affected metabolites were mainly amino acids and Amadori compounds. Insights about their biological relevance are also provided. MDPI 2021-07-09 /pmc/articles/PMC8304667/ /pubmed/34299017 http://dx.doi.org/10.3390/ijms22147399 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bernardo-Bermejo, Samuel
Sánchez-López, Elena
Tan, Lei
Benito-Martínez, Selma
Jiang, Zhengjin
Castro-Puyana, María
Lucio-Cazaña, Francisco Javier
Marina, María Luisa
Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography–Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells
title Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography–Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells
title_full Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography–Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells
title_fullStr Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography–Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells
title_full_unstemmed Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography–Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells
title_short Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography–Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells
title_sort exploratory metabolomic analysis based on reversed-phase liquid chromatography–mass spectrometry to study an in vitro model of hypoxia-induced metabolic alterations in hk-2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304667/
https://www.ncbi.nlm.nih.gov/pubmed/34299017
http://dx.doi.org/10.3390/ijms22147399
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