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Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients

Alterations in lipid metabolism mediated by oxidative stress play a key role in the process of atherosclerosis and superimposed thrombosis; these can lead to acute coronary syndrome (ACS) and acute ischemic stroke (AIS). Multiple studies have shown that the formation of atheromatous lesions is initi...

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Autores principales: Malý, Martin, Hajšl, Martin, Bechyňská, Kamila, Kučerka, Ondřej, Šrámek, Martin, Suttnar, Jiří, Hlaváčková, Alžběta, Hajšlová, Jana, Kosek, Vít
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304850/
https://www.ncbi.nlm.nih.gov/pubmed/34201850
http://dx.doi.org/10.3390/metabo11070412
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author Malý, Martin
Hajšl, Martin
Bechyňská, Kamila
Kučerka, Ondřej
Šrámek, Martin
Suttnar, Jiří
Hlaváčková, Alžběta
Hajšlová, Jana
Kosek, Vít
author_facet Malý, Martin
Hajšl, Martin
Bechyňská, Kamila
Kučerka, Ondřej
Šrámek, Martin
Suttnar, Jiří
Hlaváčková, Alžběta
Hajšlová, Jana
Kosek, Vít
author_sort Malý, Martin
collection PubMed
description Alterations in lipid metabolism mediated by oxidative stress play a key role in the process of atherosclerosis and superimposed thrombosis; these can lead to acute coronary syndrome (ACS) and acute ischemic stroke (AIS). Multiple studies have shown that the formation of atheromatous lesions is initiated by oxidation of low-density lipoproteins incorporated into the intima of the vessel wall. Here, we studied lipids in plasma samples from three cohorts: 61 patients with ACS (group A), 49 patients with AIS (group D), and 82 controls (group K). Untargeted lipidomics based on high-performance liquid chromatography coupled to mass spectrometry (UHPLC-HRMS) was employed to obtain comprehensive information on whether relationships exist between these patient categories based on lipid patterns. In addition, malondialdehyde (MDA) as a standard marker of oxidative stress was monitored. The most characteristic lipids in group K were fatty acyls of hydroxyfatty acids (FAHFAs). As expected, MDA concentrations were the lowest in group K. Our findings can better explain ongoing pathologies, both acute and chronic, with the potential for future diagnosis and treatment.
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spelling pubmed-83048502021-07-25 Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients Malý, Martin Hajšl, Martin Bechyňská, Kamila Kučerka, Ondřej Šrámek, Martin Suttnar, Jiří Hlaváčková, Alžběta Hajšlová, Jana Kosek, Vít Metabolites Article Alterations in lipid metabolism mediated by oxidative stress play a key role in the process of atherosclerosis and superimposed thrombosis; these can lead to acute coronary syndrome (ACS) and acute ischemic stroke (AIS). Multiple studies have shown that the formation of atheromatous lesions is initiated by oxidation of low-density lipoproteins incorporated into the intima of the vessel wall. Here, we studied lipids in plasma samples from three cohorts: 61 patients with ACS (group A), 49 patients with AIS (group D), and 82 controls (group K). Untargeted lipidomics based on high-performance liquid chromatography coupled to mass spectrometry (UHPLC-HRMS) was employed to obtain comprehensive information on whether relationships exist between these patient categories based on lipid patterns. In addition, malondialdehyde (MDA) as a standard marker of oxidative stress was monitored. The most characteristic lipids in group K were fatty acyls of hydroxyfatty acids (FAHFAs). As expected, MDA concentrations were the lowest in group K. Our findings can better explain ongoing pathologies, both acute and chronic, with the potential for future diagnosis and treatment. MDPI 2021-06-23 /pmc/articles/PMC8304850/ /pubmed/34201850 http://dx.doi.org/10.3390/metabo11070412 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malý, Martin
Hajšl, Martin
Bechyňská, Kamila
Kučerka, Ondřej
Šrámek, Martin
Suttnar, Jiří
Hlaváčková, Alžběta
Hajšlová, Jana
Kosek, Vít
Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients
title Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients
title_full Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients
title_fullStr Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients
title_full_unstemmed Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients
title_short Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients
title_sort lipidomic analysis to assess oxidative stress in acute coronary syndrome and acute stroke patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304850/
https://www.ncbi.nlm.nih.gov/pubmed/34201850
http://dx.doi.org/10.3390/metabo11070412
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