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Saliva Molecular Testing for SARS-CoV-2 Surveillance in Two Italian Primary Schools

Background: No evidence has so far proven a significant role of schools in SARS-CoV-2 transmission, while the negative effects of their closure on children and adolescents are well documented. Surveillance, by means of frequent students and staff testing, has been advocated in order to implement sch...

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Autores principales: Carmagnola, Daniela, Pellegrini, Gaia, Canciani, Elena, Henin, Dolaji, Perrotta, Mariachiara, Forlanini, Federica, Barcellini, Lucia, Dellavia, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304905/
https://www.ncbi.nlm.nih.gov/pubmed/34202882
http://dx.doi.org/10.3390/children8070544
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author Carmagnola, Daniela
Pellegrini, Gaia
Canciani, Elena
Henin, Dolaji
Perrotta, Mariachiara
Forlanini, Federica
Barcellini, Lucia
Dellavia, Claudia
author_facet Carmagnola, Daniela
Pellegrini, Gaia
Canciani, Elena
Henin, Dolaji
Perrotta, Mariachiara
Forlanini, Federica
Barcellini, Lucia
Dellavia, Claudia
author_sort Carmagnola, Daniela
collection PubMed
description Background: No evidence has so far proven a significant role of schools in SARS-CoV-2 transmission, while the negative effects of their closure on children and adolescents are well documented. Surveillance, by means of frequent students and staff testing, has been advocated in order to implement school safety. Our aim was to report the results of a school surveillance program for the early detection of SARS-CoV-2 infection in pre- and asymptomatic subjects, by means of molecular salivary testing (MST). Methods: School surveillance in two schools in Milan, Italy, was carried out for six weeks. Each participant received a saliva collection kit, to be self-performed. Results: 401 students and 12 teachers were enrolled, and 5 positive children in 5 different classes were observed. All the cases were asymptomatic. Their nasopharyngeal swab was positive on the same day in four cases, while in one case it resulted negative on the same day and positive 3 days later. In one positive case, the whole family was set under surveillance. The positive child did not develop symptoms and no family member was infected. Conclusions: MST might represent an efficient way to actively survey communities in order to detect asymptomatic cases, thus limiting SARS-CoV-2 transmission.
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spelling pubmed-83049052021-07-25 Saliva Molecular Testing for SARS-CoV-2 Surveillance in Two Italian Primary Schools Carmagnola, Daniela Pellegrini, Gaia Canciani, Elena Henin, Dolaji Perrotta, Mariachiara Forlanini, Federica Barcellini, Lucia Dellavia, Claudia Children (Basel) Article Background: No evidence has so far proven a significant role of schools in SARS-CoV-2 transmission, while the negative effects of their closure on children and adolescents are well documented. Surveillance, by means of frequent students and staff testing, has been advocated in order to implement school safety. Our aim was to report the results of a school surveillance program for the early detection of SARS-CoV-2 infection in pre- and asymptomatic subjects, by means of molecular salivary testing (MST). Methods: School surveillance in two schools in Milan, Italy, was carried out for six weeks. Each participant received a saliva collection kit, to be self-performed. Results: 401 students and 12 teachers were enrolled, and 5 positive children in 5 different classes were observed. All the cases were asymptomatic. Their nasopharyngeal swab was positive on the same day in four cases, while in one case it resulted negative on the same day and positive 3 days later. In one positive case, the whole family was set under surveillance. The positive child did not develop symptoms and no family member was infected. Conclusions: MST might represent an efficient way to actively survey communities in order to detect asymptomatic cases, thus limiting SARS-CoV-2 transmission. MDPI 2021-06-24 /pmc/articles/PMC8304905/ /pubmed/34202882 http://dx.doi.org/10.3390/children8070544 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carmagnola, Daniela
Pellegrini, Gaia
Canciani, Elena
Henin, Dolaji
Perrotta, Mariachiara
Forlanini, Federica
Barcellini, Lucia
Dellavia, Claudia
Saliva Molecular Testing for SARS-CoV-2 Surveillance in Two Italian Primary Schools
title Saliva Molecular Testing for SARS-CoV-2 Surveillance in Two Italian Primary Schools
title_full Saliva Molecular Testing for SARS-CoV-2 Surveillance in Two Italian Primary Schools
title_fullStr Saliva Molecular Testing for SARS-CoV-2 Surveillance in Two Italian Primary Schools
title_full_unstemmed Saliva Molecular Testing for SARS-CoV-2 Surveillance in Two Italian Primary Schools
title_short Saliva Molecular Testing for SARS-CoV-2 Surveillance in Two Italian Primary Schools
title_sort saliva molecular testing for sars-cov-2 surveillance in two italian primary schools
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304905/
https://www.ncbi.nlm.nih.gov/pubmed/34202882
http://dx.doi.org/10.3390/children8070544
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