Cargando…
Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay
SIMPLE SUMMARY: The Neotropical brown stink bug Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) is currently one of the major soybean pests in Brazil, causing considerable and often irreversible damage to the crop. The main control strategy for regulating brown stink bug populations has been...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305007/ https://www.ncbi.nlm.nih.gov/pubmed/34357259 http://dx.doi.org/10.3390/insects12070599 |
_version_ | 1783727471354445824 |
---|---|
author | Tibola, Cristiane Maria Silva, Leandro Sgubin, Fernanda Omoto, Celso |
author_facet | Tibola, Cristiane Maria Silva, Leandro Sgubin, Fernanda Omoto, Celso |
author_sort | Tibola, Cristiane Maria |
collection | PubMed |
description | SIMPLE SUMMARY: The Neotropical brown stink bug Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) is currently one of the major soybean pests in Brazil, causing considerable and often irreversible damage to the crop. The main control strategy for regulating brown stink bug populations has been the use of insecticides, especially those belonging to the neonicotinoid class; however, the spraying of these insecticides does not achieve the desired control efficiency in some locations. Therefore, to improve the programs for detecting and monitoring E. heros resistance to insecticides, a new ingestion bioassay method by liquid diet encapsulation was evaluated in comparison with traditional bioassay methods. The new bioassay method of ingestion by encapsulation is very promising for detecting and monitoring resistance of E. heros populations to neonicotinoid insecticides. ABSTRACT: The novel ingestion bioassay method was developed for detecting and monitoring resistance of Euschistus heros by encapsulating an artificial liquid diet using Parafilm(®). This methodology was compared with the tarsal contact (vial test) and topical application methods for thiamethoxam, imidacloprid, and lambda-cyhalothrin. The best bioassay method for the neonicotinoid insecticides thiamethoxam and imidacloprid was ingestion. For pyrethroid insecticide lambda-cyhalothrin, the best result was obtained by topical application. Using the best bioassay method for each insecticide, the susceptibility to these insecticides was monitored in 30 populations of E. heros collected from soybean crops in Brazil from 2018 to 2020. High variations in susceptibility to thiamethoxam (resistance ratios, 1.6–22 times), imidacloprid (resistance ratios, 1.6–22 times), and lambda-cyhalothrin (resistance ratios, 5–40 times) were detected among the evaluated E. heros populations. In order to monitor the susceptibility of E. heros to insecticides, diagnostic concentrations were defined based on the LC(99) of the susceptible reference population: 5.65 µL of a.i./mL for thiamethoxam, 12.45 µL of a.i./mL for imidacloprid, and 0.20 µg of a.i./insect for lambda-cyhalothrin. Subsequently, we select an E. heros strain resistant to neonicotinoid insecticides and another to lambda-cyhalothrin. The resistance ratios obtained after seven selection cycles were 66, 41 and 44 times for thiamethoxam, imidacloprid and lambda-cyhalothrin, respectively. |
format | Online Article Text |
id | pubmed-8305007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83050072021-07-25 Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay Tibola, Cristiane Maria Silva, Leandro Sgubin, Fernanda Omoto, Celso Insects Article SIMPLE SUMMARY: The Neotropical brown stink bug Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) is currently one of the major soybean pests in Brazil, causing considerable and often irreversible damage to the crop. The main control strategy for regulating brown stink bug populations has been the use of insecticides, especially those belonging to the neonicotinoid class; however, the spraying of these insecticides does not achieve the desired control efficiency in some locations. Therefore, to improve the programs for detecting and monitoring E. heros resistance to insecticides, a new ingestion bioassay method by liquid diet encapsulation was evaluated in comparison with traditional bioassay methods. The new bioassay method of ingestion by encapsulation is very promising for detecting and monitoring resistance of E. heros populations to neonicotinoid insecticides. ABSTRACT: The novel ingestion bioassay method was developed for detecting and monitoring resistance of Euschistus heros by encapsulating an artificial liquid diet using Parafilm(®). This methodology was compared with the tarsal contact (vial test) and topical application methods for thiamethoxam, imidacloprid, and lambda-cyhalothrin. The best bioassay method for the neonicotinoid insecticides thiamethoxam and imidacloprid was ingestion. For pyrethroid insecticide lambda-cyhalothrin, the best result was obtained by topical application. Using the best bioassay method for each insecticide, the susceptibility to these insecticides was monitored in 30 populations of E. heros collected from soybean crops in Brazil from 2018 to 2020. High variations in susceptibility to thiamethoxam (resistance ratios, 1.6–22 times), imidacloprid (resistance ratios, 1.6–22 times), and lambda-cyhalothrin (resistance ratios, 5–40 times) were detected among the evaluated E. heros populations. In order to monitor the susceptibility of E. heros to insecticides, diagnostic concentrations were defined based on the LC(99) of the susceptible reference population: 5.65 µL of a.i./mL for thiamethoxam, 12.45 µL of a.i./mL for imidacloprid, and 0.20 µg of a.i./insect for lambda-cyhalothrin. Subsequently, we select an E. heros strain resistant to neonicotinoid insecticides and another to lambda-cyhalothrin. The resistance ratios obtained after seven selection cycles were 66, 41 and 44 times for thiamethoxam, imidacloprid and lambda-cyhalothrin, respectively. MDPI 2021-07-01 /pmc/articles/PMC8305007/ /pubmed/34357259 http://dx.doi.org/10.3390/insects12070599 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tibola, Cristiane Maria Silva, Leandro Sgubin, Fernanda Omoto, Celso Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
title | Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
title_full | Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
title_fullStr | Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
title_full_unstemmed | Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
title_short | Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
title_sort | monitoring resistance of euschistus heros (fabricius) (hemiptera: pentatomidae) to insecticides by using encapsulated artificial diet bioassay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305007/ https://www.ncbi.nlm.nih.gov/pubmed/34357259 http://dx.doi.org/10.3390/insects12070599 |
work_keys_str_mv | AT tibolacristianemaria monitoringresistanceofeuschistusherosfabriciushemipterapentatomidaetoinsecticidesbyusingencapsulatedartificialdietbioassay AT silvaleandro monitoringresistanceofeuschistusherosfabriciushemipterapentatomidaetoinsecticidesbyusingencapsulatedartificialdietbioassay AT sgubinfernanda monitoringresistanceofeuschistusherosfabriciushemipterapentatomidaetoinsecticidesbyusingencapsulatedartificialdietbioassay AT omotocelso monitoringresistanceofeuschistusherosfabriciushemipterapentatomidaetoinsecticidesbyusingencapsulatedartificialdietbioassay |