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Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress

Staphylococcus aureus is a common food pathogen and has a strong tolerance to environmental stress. Here, the biofilm formation of S. aureus strains after cold stress for 24 weeks were investigated. It was found that the biofilm formation of S. aureus CICC 21600, CICC 22942, W1, W3, and C1 cells was...

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Autores principales: Qiao, Jiaju, Zheng, Liping, Lu, Zhaoxin, Meng, Fanqiang, Bie, Xiaomei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305040/
https://www.ncbi.nlm.nih.gov/pubmed/34361968
http://dx.doi.org/10.3390/microorganisms9071534
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author Qiao, Jiaju
Zheng, Liping
Lu, Zhaoxin
Meng, Fanqiang
Bie, Xiaomei
author_facet Qiao, Jiaju
Zheng, Liping
Lu, Zhaoxin
Meng, Fanqiang
Bie, Xiaomei
author_sort Qiao, Jiaju
collection PubMed
description Staphylococcus aureus is a common food pathogen and has a strong tolerance to environmental stress. Here, the biofilm formation of S. aureus strains after cold stress for 24 weeks were investigated. It was found that the biofilm formation of S. aureus CICC 21600, CICC 22942, W1, W3, and C1 cells was enhanced after cold stress for 20 weeks. What is more, the mRNA levels of the clfA, icaA, icaB, icaC or icaD genes in these strains were increased for >2-fold. The increased gene transcription levels were consistent with the increase in the polysaccharide content in the biofilm matrix of these S. aureus strains after cold stress. Meanwhile, hydrophobicity and the adhesion proteins also played a role in the formation of biofilms. The biofilm of S. aureus cells can be effectively degraded by snailase and proteinase K (125 µg/mL + 20 µg/mL) mixture. In summary, S. aureus frozen at −20 °C for 12 to 20 weeks is still a potential hazard. Food factory equipment should be cleaned in a timely manner to avoid outbreaks of foodborne pathogenic bacteria due to contamination.
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spelling pubmed-83050402021-07-25 Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress Qiao, Jiaju Zheng, Liping Lu, Zhaoxin Meng, Fanqiang Bie, Xiaomei Microorganisms Article Staphylococcus aureus is a common food pathogen and has a strong tolerance to environmental stress. Here, the biofilm formation of S. aureus strains after cold stress for 24 weeks were investigated. It was found that the biofilm formation of S. aureus CICC 21600, CICC 22942, W1, W3, and C1 cells was enhanced after cold stress for 20 weeks. What is more, the mRNA levels of the clfA, icaA, icaB, icaC or icaD genes in these strains were increased for >2-fold. The increased gene transcription levels were consistent with the increase in the polysaccharide content in the biofilm matrix of these S. aureus strains after cold stress. Meanwhile, hydrophobicity and the adhesion proteins also played a role in the formation of biofilms. The biofilm of S. aureus cells can be effectively degraded by snailase and proteinase K (125 µg/mL + 20 µg/mL) mixture. In summary, S. aureus frozen at −20 °C for 12 to 20 weeks is still a potential hazard. Food factory equipment should be cleaned in a timely manner to avoid outbreaks of foodborne pathogenic bacteria due to contamination. MDPI 2021-07-19 /pmc/articles/PMC8305040/ /pubmed/34361968 http://dx.doi.org/10.3390/microorganisms9071534 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qiao, Jiaju
Zheng, Liping
Lu, Zhaoxin
Meng, Fanqiang
Bie, Xiaomei
Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress
title Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress
title_full Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress
title_fullStr Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress
title_full_unstemmed Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress
title_short Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress
title_sort research on the biofilm formation of staphylococcus aureus after cold stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305040/
https://www.ncbi.nlm.nih.gov/pubmed/34361968
http://dx.doi.org/10.3390/microorganisms9071534
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