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Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress
Staphylococcus aureus is a common food pathogen and has a strong tolerance to environmental stress. Here, the biofilm formation of S. aureus strains after cold stress for 24 weeks were investigated. It was found that the biofilm formation of S. aureus CICC 21600, CICC 22942, W1, W3, and C1 cells was...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305040/ https://www.ncbi.nlm.nih.gov/pubmed/34361968 http://dx.doi.org/10.3390/microorganisms9071534 |
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author | Qiao, Jiaju Zheng, Liping Lu, Zhaoxin Meng, Fanqiang Bie, Xiaomei |
author_facet | Qiao, Jiaju Zheng, Liping Lu, Zhaoxin Meng, Fanqiang Bie, Xiaomei |
author_sort | Qiao, Jiaju |
collection | PubMed |
description | Staphylococcus aureus is a common food pathogen and has a strong tolerance to environmental stress. Here, the biofilm formation of S. aureus strains after cold stress for 24 weeks were investigated. It was found that the biofilm formation of S. aureus CICC 21600, CICC 22942, W1, W3, and C1 cells was enhanced after cold stress for 20 weeks. What is more, the mRNA levels of the clfA, icaA, icaB, icaC or icaD genes in these strains were increased for >2-fold. The increased gene transcription levels were consistent with the increase in the polysaccharide content in the biofilm matrix of these S. aureus strains after cold stress. Meanwhile, hydrophobicity and the adhesion proteins also played a role in the formation of biofilms. The biofilm of S. aureus cells can be effectively degraded by snailase and proteinase K (125 µg/mL + 20 µg/mL) mixture. In summary, S. aureus frozen at −20 °C for 12 to 20 weeks is still a potential hazard. Food factory equipment should be cleaned in a timely manner to avoid outbreaks of foodborne pathogenic bacteria due to contamination. |
format | Online Article Text |
id | pubmed-8305040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83050402021-07-25 Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress Qiao, Jiaju Zheng, Liping Lu, Zhaoxin Meng, Fanqiang Bie, Xiaomei Microorganisms Article Staphylococcus aureus is a common food pathogen and has a strong tolerance to environmental stress. Here, the biofilm formation of S. aureus strains after cold stress for 24 weeks were investigated. It was found that the biofilm formation of S. aureus CICC 21600, CICC 22942, W1, W3, and C1 cells was enhanced after cold stress for 20 weeks. What is more, the mRNA levels of the clfA, icaA, icaB, icaC or icaD genes in these strains were increased for >2-fold. The increased gene transcription levels were consistent with the increase in the polysaccharide content in the biofilm matrix of these S. aureus strains after cold stress. Meanwhile, hydrophobicity and the adhesion proteins also played a role in the formation of biofilms. The biofilm of S. aureus cells can be effectively degraded by snailase and proteinase K (125 µg/mL + 20 µg/mL) mixture. In summary, S. aureus frozen at −20 °C for 12 to 20 weeks is still a potential hazard. Food factory equipment should be cleaned in a timely manner to avoid outbreaks of foodborne pathogenic bacteria due to contamination. MDPI 2021-07-19 /pmc/articles/PMC8305040/ /pubmed/34361968 http://dx.doi.org/10.3390/microorganisms9071534 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Qiao, Jiaju Zheng, Liping Lu, Zhaoxin Meng, Fanqiang Bie, Xiaomei Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress |
title | Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress |
title_full | Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress |
title_fullStr | Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress |
title_full_unstemmed | Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress |
title_short | Research on the Biofilm Formation of Staphylococcus aureus after Cold Stress |
title_sort | research on the biofilm formation of staphylococcus aureus after cold stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305040/ https://www.ncbi.nlm.nih.gov/pubmed/34361968 http://dx.doi.org/10.3390/microorganisms9071534 |
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