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Optimizing Transformation Frequency of Cryptococcus neoformans and Cryptococcus gattii Using Agrobacterium tumefaciens

The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell wall damage, and agar concentration. Agar con...

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Detalles Bibliográficos
Autores principales:	Fu, Jianmin, Brockman, Nohelli E., Wickes, Brian L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Review
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305055/ https://www.ncbi.nlm.nih.gov/pubmed/34209781 http://dx.doi.org/10.3390/jof7070520

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