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CRISPR/Cas9-Mediated Specific Integration of Fat-1 and IGF-1 at the pRosa26 Locus

Many researchers have focused on knock-in pigs for site-specific integration, but little attention has been given to genetically modified pigs with the targeted integration of multiple recombinant genes. To establish a multigene targeted knock-in editing system, we used the internal ribosome entry s...

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Detalles Bibliográficos
Autores principales: You, Wenni, Li, Mengjing, Qi, Yilin, Wang, Yanbing, Chen, Yiwu, Liu, Ying, Li, Li, Ouyang, Hongsheng, Pang, Daxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305104/
https://www.ncbi.nlm.nih.gov/pubmed/34356043
http://dx.doi.org/10.3390/genes12071027