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Effects of the Developmental Regulator BOLITA on the Plant Metabolome
Transcription factors are important regulators of gene expression. They can orchestrate the activation or repression of hundreds or thousands of genes and control diverse processes in a coordinated way. This work explores the effect of a master regulator of plant development, BOLITA (BOL), in plant...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305173/ https://www.ncbi.nlm.nih.gov/pubmed/34209960 http://dx.doi.org/10.3390/genes12070995 |
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author | Lazcano-Ramírez, Hugo Gerardo Gamboa-Becerra, Roberto García-López, Irving J. Montes, Ricardo A. Chávez Díaz-Ramírez, David de la Vega, Octavio Martínez Ordaz-Ortíz, José Juan de Folter, Stefan Tiessen-Favier, Axel Winkler, Robert Marsch-Martínez, Nayelli |
author_facet | Lazcano-Ramírez, Hugo Gerardo Gamboa-Becerra, Roberto García-López, Irving J. Montes, Ricardo A. Chávez Díaz-Ramírez, David de la Vega, Octavio Martínez Ordaz-Ortíz, José Juan de Folter, Stefan Tiessen-Favier, Axel Winkler, Robert Marsch-Martínez, Nayelli |
author_sort | Lazcano-Ramírez, Hugo Gerardo |
collection | PubMed |
description | Transcription factors are important regulators of gene expression. They can orchestrate the activation or repression of hundreds or thousands of genes and control diverse processes in a coordinated way. This work explores the effect of a master regulator of plant development, BOLITA (BOL), in plant metabolism, with a special focus on specialized metabolism. For this, we used an Arabidopsis thaliana line in which the transcription factor activity can be induced. Fingerprinting metabolomic analyses of whole plantlets were performed at different times after induction. After 96 h, all induced replicas clustered as a single group, in contrast with all controls which did not cluster. Metabolomic analyses of shoot and root tissues enabled the putative identification of differentially accumulated metabolites in each tissue. Finally, the analysis of global gene expression in induced vs. non-induced root samples, together with enrichment analyses, allowed the identification of enriched metabolic pathways among the differentially expressed genes and accumulated metabolites after the induction. We concluded that the induction of BOL activity can modify the Arabidopsis metabolome. Future work should investigate whether its action is direct or indirect, and the implications of the metabolic changes for development regulation and bioprospection. |
format | Online Article Text |
id | pubmed-8305173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83051732021-07-25 Effects of the Developmental Regulator BOLITA on the Plant Metabolome Lazcano-Ramírez, Hugo Gerardo Gamboa-Becerra, Roberto García-López, Irving J. Montes, Ricardo A. Chávez Díaz-Ramírez, David de la Vega, Octavio Martínez Ordaz-Ortíz, José Juan de Folter, Stefan Tiessen-Favier, Axel Winkler, Robert Marsch-Martínez, Nayelli Genes (Basel) Article Transcription factors are important regulators of gene expression. They can orchestrate the activation or repression of hundreds or thousands of genes and control diverse processes in a coordinated way. This work explores the effect of a master regulator of plant development, BOLITA (BOL), in plant metabolism, with a special focus on specialized metabolism. For this, we used an Arabidopsis thaliana line in which the transcription factor activity can be induced. Fingerprinting metabolomic analyses of whole plantlets were performed at different times after induction. After 96 h, all induced replicas clustered as a single group, in contrast with all controls which did not cluster. Metabolomic analyses of shoot and root tissues enabled the putative identification of differentially accumulated metabolites in each tissue. Finally, the analysis of global gene expression in induced vs. non-induced root samples, together with enrichment analyses, allowed the identification of enriched metabolic pathways among the differentially expressed genes and accumulated metabolites after the induction. We concluded that the induction of BOL activity can modify the Arabidopsis metabolome. Future work should investigate whether its action is direct or indirect, and the implications of the metabolic changes for development regulation and bioprospection. MDPI 2021-06-29 /pmc/articles/PMC8305173/ /pubmed/34209960 http://dx.doi.org/10.3390/genes12070995 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lazcano-Ramírez, Hugo Gerardo Gamboa-Becerra, Roberto García-López, Irving J. Montes, Ricardo A. Chávez Díaz-Ramírez, David de la Vega, Octavio Martínez Ordaz-Ortíz, José Juan de Folter, Stefan Tiessen-Favier, Axel Winkler, Robert Marsch-Martínez, Nayelli Effects of the Developmental Regulator BOLITA on the Plant Metabolome |
title | Effects of the Developmental Regulator BOLITA on the Plant Metabolome |
title_full | Effects of the Developmental Regulator BOLITA on the Plant Metabolome |
title_fullStr | Effects of the Developmental Regulator BOLITA on the Plant Metabolome |
title_full_unstemmed | Effects of the Developmental Regulator BOLITA on the Plant Metabolome |
title_short | Effects of the Developmental Regulator BOLITA on the Plant Metabolome |
title_sort | effects of the developmental regulator bolita on the plant metabolome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305173/ https://www.ncbi.nlm.nih.gov/pubmed/34209960 http://dx.doi.org/10.3390/genes12070995 |
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