Cargando…

Effects of the Developmental Regulator BOLITA on the Plant Metabolome

Transcription factors are important regulators of gene expression. They can orchestrate the activation or repression of hundreds or thousands of genes and control diverse processes in a coordinated way. This work explores the effect of a master regulator of plant development, BOLITA (BOL), in plant...

Descripción completa

Detalles Bibliográficos
Autores principales: Lazcano-Ramírez, Hugo Gerardo, Gamboa-Becerra, Roberto, García-López, Irving J., Montes, Ricardo A. Chávez, Díaz-Ramírez, David, de la Vega, Octavio Martínez, Ordaz-Ortíz, José Juan, de Folter, Stefan, Tiessen-Favier, Axel, Winkler, Robert, Marsch-Martínez, Nayelli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305173/
https://www.ncbi.nlm.nih.gov/pubmed/34209960
http://dx.doi.org/10.3390/genes12070995
_version_ 1783727510858498048
author Lazcano-Ramírez, Hugo Gerardo
Gamboa-Becerra, Roberto
García-López, Irving J.
Montes, Ricardo A. Chávez
Díaz-Ramírez, David
de la Vega, Octavio Martínez
Ordaz-Ortíz, José Juan
de Folter, Stefan
Tiessen-Favier, Axel
Winkler, Robert
Marsch-Martínez, Nayelli
author_facet Lazcano-Ramírez, Hugo Gerardo
Gamboa-Becerra, Roberto
García-López, Irving J.
Montes, Ricardo A. Chávez
Díaz-Ramírez, David
de la Vega, Octavio Martínez
Ordaz-Ortíz, José Juan
de Folter, Stefan
Tiessen-Favier, Axel
Winkler, Robert
Marsch-Martínez, Nayelli
author_sort Lazcano-Ramírez, Hugo Gerardo
collection PubMed
description Transcription factors are important regulators of gene expression. They can orchestrate the activation or repression of hundreds or thousands of genes and control diverse processes in a coordinated way. This work explores the effect of a master regulator of plant development, BOLITA (BOL), in plant metabolism, with a special focus on specialized metabolism. For this, we used an Arabidopsis thaliana line in which the transcription factor activity can be induced. Fingerprinting metabolomic analyses of whole plantlets were performed at different times after induction. After 96 h, all induced replicas clustered as a single group, in contrast with all controls which did not cluster. Metabolomic analyses of shoot and root tissues enabled the putative identification of differentially accumulated metabolites in each tissue. Finally, the analysis of global gene expression in induced vs. non-induced root samples, together with enrichment analyses, allowed the identification of enriched metabolic pathways among the differentially expressed genes and accumulated metabolites after the induction. We concluded that the induction of BOL activity can modify the Arabidopsis metabolome. Future work should investigate whether its action is direct or indirect, and the implications of the metabolic changes for development regulation and bioprospection.
format Online
Article
Text
id pubmed-8305173
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83051732021-07-25 Effects of the Developmental Regulator BOLITA on the Plant Metabolome Lazcano-Ramírez, Hugo Gerardo Gamboa-Becerra, Roberto García-López, Irving J. Montes, Ricardo A. Chávez Díaz-Ramírez, David de la Vega, Octavio Martínez Ordaz-Ortíz, José Juan de Folter, Stefan Tiessen-Favier, Axel Winkler, Robert Marsch-Martínez, Nayelli Genes (Basel) Article Transcription factors are important regulators of gene expression. They can orchestrate the activation or repression of hundreds or thousands of genes and control diverse processes in a coordinated way. This work explores the effect of a master regulator of plant development, BOLITA (BOL), in plant metabolism, with a special focus on specialized metabolism. For this, we used an Arabidopsis thaliana line in which the transcription factor activity can be induced. Fingerprinting metabolomic analyses of whole plantlets were performed at different times after induction. After 96 h, all induced replicas clustered as a single group, in contrast with all controls which did not cluster. Metabolomic analyses of shoot and root tissues enabled the putative identification of differentially accumulated metabolites in each tissue. Finally, the analysis of global gene expression in induced vs. non-induced root samples, together with enrichment analyses, allowed the identification of enriched metabolic pathways among the differentially expressed genes and accumulated metabolites after the induction. We concluded that the induction of BOL activity can modify the Arabidopsis metabolome. Future work should investigate whether its action is direct or indirect, and the implications of the metabolic changes for development regulation and bioprospection. MDPI 2021-06-29 /pmc/articles/PMC8305173/ /pubmed/34209960 http://dx.doi.org/10.3390/genes12070995 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lazcano-Ramírez, Hugo Gerardo
Gamboa-Becerra, Roberto
García-López, Irving J.
Montes, Ricardo A. Chávez
Díaz-Ramírez, David
de la Vega, Octavio Martínez
Ordaz-Ortíz, José Juan
de Folter, Stefan
Tiessen-Favier, Axel
Winkler, Robert
Marsch-Martínez, Nayelli
Effects of the Developmental Regulator BOLITA on the Plant Metabolome
title Effects of the Developmental Regulator BOLITA on the Plant Metabolome
title_full Effects of the Developmental Regulator BOLITA on the Plant Metabolome
title_fullStr Effects of the Developmental Regulator BOLITA on the Plant Metabolome
title_full_unstemmed Effects of the Developmental Regulator BOLITA on the Plant Metabolome
title_short Effects of the Developmental Regulator BOLITA on the Plant Metabolome
title_sort effects of the developmental regulator bolita on the plant metabolome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305173/
https://www.ncbi.nlm.nih.gov/pubmed/34209960
http://dx.doi.org/10.3390/genes12070995
work_keys_str_mv AT lazcanoramirezhugogerardo effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT gamboabecerraroberto effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT garcialopezirvingj effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT montesricardoachavez effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT diazramirezdavid effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT delavegaoctaviomartinez effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT ordazortizjosejuan effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT defolterstefan effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT tiessenfavieraxel effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT winklerrobert effectsofthedevelopmentalregulatorbolitaontheplantmetabolome
AT marschmartineznayelli effectsofthedevelopmentalregulatorbolitaontheplantmetabolome