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Cerebrospinal Fluid Chitinases as Biomarkers for Amyotrophic Lateral Sclerosis
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative neuromuscular disease that affects motor neurons controlling voluntary muscles. Survival is usually 2–5 years after onset, and death occurs due to respiratory failure. The identification of biomarkers would be very useful to help in disease...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305219/ https://www.ncbi.nlm.nih.gov/pubmed/34359293 http://dx.doi.org/10.3390/diagnostics11071210 |
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author | Costa, Júlia Gromicho, Marta Pronto-Laborinho, Ana Almeida, Conceição Gomes, Ricardo A. Guerreiro, Ana C. L. Oliva, Abel Pinto, Susana de Carvalho, Mamede |
author_facet | Costa, Júlia Gromicho, Marta Pronto-Laborinho, Ana Almeida, Conceição Gomes, Ricardo A. Guerreiro, Ana C. L. Oliva, Abel Pinto, Susana de Carvalho, Mamede |
author_sort | Costa, Júlia |
collection | PubMed |
description | Amyotrophic lateral sclerosis (ALS) is a neurodegenerative neuromuscular disease that affects motor neurons controlling voluntary muscles. Survival is usually 2–5 years after onset, and death occurs due to respiratory failure. The identification of biomarkers would be very useful to help in disease diagnosis and for patient stratification based on, e.g., progression rate, with implications in therapeutic trials. Neurofilaments constitute already-promising markers for ALS and, recently, chitinases have emerged as novel marker targets for the disease. Here, we investigated cerebrospinal fluid (CSF) chitinases as potential markers for ALS. Chitotriosidase (CHIT1), chitinase-3-like protein 1 (CHI3L1), chitinase-3-like protein 2 (CHI3L2) and the benchmark marker phosphoneurofilament heavy chain (pNFH) were quantified by an enzyme-linked immunosorbent assay (ELISA) from the CSF of 34 ALS patients and 24 control patients with other neurological diseases. CSF was also analyzed by UHPLC-mass spectrometry. All three chitinases, as well as pNFH, were found to correlate with disease progression rate. Furthermore, CHIT1 was elevated in ALS patients with high diagnostic performance, as was pNFH. On the other hand, CHIT1 correlated with forced vital capacity (FVC). The three chitinases correlated with pNFH, indicating a relation between degeneration and neuroinflammation. In conclusion, our results supported the value of CHIT1 as a diagnostic and progression rate biomarker, and its potential as respiratory function marker. The results opened novel perspectives to explore chitinases as biomarkers and their functional relevance in ALS. |
format | Online Article Text |
id | pubmed-8305219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83052192021-07-25 Cerebrospinal Fluid Chitinases as Biomarkers for Amyotrophic Lateral Sclerosis Costa, Júlia Gromicho, Marta Pronto-Laborinho, Ana Almeida, Conceição Gomes, Ricardo A. Guerreiro, Ana C. L. Oliva, Abel Pinto, Susana de Carvalho, Mamede Diagnostics (Basel) Article Amyotrophic lateral sclerosis (ALS) is a neurodegenerative neuromuscular disease that affects motor neurons controlling voluntary muscles. Survival is usually 2–5 years after onset, and death occurs due to respiratory failure. The identification of biomarkers would be very useful to help in disease diagnosis and for patient stratification based on, e.g., progression rate, with implications in therapeutic trials. Neurofilaments constitute already-promising markers for ALS and, recently, chitinases have emerged as novel marker targets for the disease. Here, we investigated cerebrospinal fluid (CSF) chitinases as potential markers for ALS. Chitotriosidase (CHIT1), chitinase-3-like protein 1 (CHI3L1), chitinase-3-like protein 2 (CHI3L2) and the benchmark marker phosphoneurofilament heavy chain (pNFH) were quantified by an enzyme-linked immunosorbent assay (ELISA) from the CSF of 34 ALS patients and 24 control patients with other neurological diseases. CSF was also analyzed by UHPLC-mass spectrometry. All three chitinases, as well as pNFH, were found to correlate with disease progression rate. Furthermore, CHIT1 was elevated in ALS patients with high diagnostic performance, as was pNFH. On the other hand, CHIT1 correlated with forced vital capacity (FVC). The three chitinases correlated with pNFH, indicating a relation between degeneration and neuroinflammation. In conclusion, our results supported the value of CHIT1 as a diagnostic and progression rate biomarker, and its potential as respiratory function marker. The results opened novel perspectives to explore chitinases as biomarkers and their functional relevance in ALS. MDPI 2021-07-05 /pmc/articles/PMC8305219/ /pubmed/34359293 http://dx.doi.org/10.3390/diagnostics11071210 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Costa, Júlia Gromicho, Marta Pronto-Laborinho, Ana Almeida, Conceição Gomes, Ricardo A. Guerreiro, Ana C. L. Oliva, Abel Pinto, Susana de Carvalho, Mamede Cerebrospinal Fluid Chitinases as Biomarkers for Amyotrophic Lateral Sclerosis |
title | Cerebrospinal Fluid Chitinases as Biomarkers for Amyotrophic Lateral Sclerosis |
title_full | Cerebrospinal Fluid Chitinases as Biomarkers for Amyotrophic Lateral Sclerosis |
title_fullStr | Cerebrospinal Fluid Chitinases as Biomarkers for Amyotrophic Lateral Sclerosis |
title_full_unstemmed | Cerebrospinal Fluid Chitinases as Biomarkers for Amyotrophic Lateral Sclerosis |
title_short | Cerebrospinal Fluid Chitinases as Biomarkers for Amyotrophic Lateral Sclerosis |
title_sort | cerebrospinal fluid chitinases as biomarkers for amyotrophic lateral sclerosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305219/ https://www.ncbi.nlm.nih.gov/pubmed/34359293 http://dx.doi.org/10.3390/diagnostics11071210 |
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