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Identification of Amyloidogenic Regions in Pseudomonas aeruginosa Ribosomal S1 Protein

Bacterial S1 protein is a functionally important ribosomal protein. It is a part of the 30S ribosomal subunit and is also able to interact with mRNA and tmRNA. An important feature of the S1 protein family is a strong tendency towards aggregation. To study the amyloidogenic properties of S1, we isol...

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Autores principales: Grishin, Sergei Y., Dzhus, Ulyana F., Glukhov, Anatoly S., Selivanova, Olga M., Surin, Alexey K., Galzitskaya, Oxana V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305250/
https://www.ncbi.nlm.nih.gov/pubmed/34298910
http://dx.doi.org/10.3390/ijms22147291
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author Grishin, Sergei Y.
Dzhus, Ulyana F.
Glukhov, Anatoly S.
Selivanova, Olga M.
Surin, Alexey K.
Galzitskaya, Oxana V.
author_facet Grishin, Sergei Y.
Dzhus, Ulyana F.
Glukhov, Anatoly S.
Selivanova, Olga M.
Surin, Alexey K.
Galzitskaya, Oxana V.
author_sort Grishin, Sergei Y.
collection PubMed
description Bacterial S1 protein is a functionally important ribosomal protein. It is a part of the 30S ribosomal subunit and is also able to interact with mRNA and tmRNA. An important feature of the S1 protein family is a strong tendency towards aggregation. To study the amyloidogenic properties of S1, we isolated and purified the recombinant ribosomal S1 protein of Pseudomonas aeruginosa. Using the FoldAmyloid, Waltz, Pasta 2.0, and AGGRESCAN programs, amyloidogenic regions of the protein were predicted, which play a key role in its aggregation. The method of limited proteolysis in combination with high performance liquid chromatography and mass spectrometric analysis of the products, made it possible to identify regions of the S1 protein from P. aeruginosa that are protected from the action of proteinase K, trypsin, and chymotrypsin. Sequences of theoretically predicted and experimentally identified amyloidogenic regions were used to synthesize four peptides, three of which demonstrated the ability to form amyloid-like fibrils, as shown by electron microscopy and fluorescence spectroscopy. The identified amyloidogenic sites can further serve as a basis for the development of new antibacterial peptides against the pathogenic microorganism P. aeruginosa.
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spelling pubmed-83052502021-07-25 Identification of Amyloidogenic Regions in Pseudomonas aeruginosa Ribosomal S1 Protein Grishin, Sergei Y. Dzhus, Ulyana F. Glukhov, Anatoly S. Selivanova, Olga M. Surin, Alexey K. Galzitskaya, Oxana V. Int J Mol Sci Article Bacterial S1 protein is a functionally important ribosomal protein. It is a part of the 30S ribosomal subunit and is also able to interact with mRNA and tmRNA. An important feature of the S1 protein family is a strong tendency towards aggregation. To study the amyloidogenic properties of S1, we isolated and purified the recombinant ribosomal S1 protein of Pseudomonas aeruginosa. Using the FoldAmyloid, Waltz, Pasta 2.0, and AGGRESCAN programs, amyloidogenic regions of the protein were predicted, which play a key role in its aggregation. The method of limited proteolysis in combination with high performance liquid chromatography and mass spectrometric analysis of the products, made it possible to identify regions of the S1 protein from P. aeruginosa that are protected from the action of proteinase K, trypsin, and chymotrypsin. Sequences of theoretically predicted and experimentally identified amyloidogenic regions were used to synthesize four peptides, three of which demonstrated the ability to form amyloid-like fibrils, as shown by electron microscopy and fluorescence spectroscopy. The identified amyloidogenic sites can further serve as a basis for the development of new antibacterial peptides against the pathogenic microorganism P. aeruginosa. MDPI 2021-07-07 /pmc/articles/PMC8305250/ /pubmed/34298910 http://dx.doi.org/10.3390/ijms22147291 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grishin, Sergei Y.
Dzhus, Ulyana F.
Glukhov, Anatoly S.
Selivanova, Olga M.
Surin, Alexey K.
Galzitskaya, Oxana V.
Identification of Amyloidogenic Regions in Pseudomonas aeruginosa Ribosomal S1 Protein
title Identification of Amyloidogenic Regions in Pseudomonas aeruginosa Ribosomal S1 Protein
title_full Identification of Amyloidogenic Regions in Pseudomonas aeruginosa Ribosomal S1 Protein
title_fullStr Identification of Amyloidogenic Regions in Pseudomonas aeruginosa Ribosomal S1 Protein
title_full_unstemmed Identification of Amyloidogenic Regions in Pseudomonas aeruginosa Ribosomal S1 Protein
title_short Identification of Amyloidogenic Regions in Pseudomonas aeruginosa Ribosomal S1 Protein
title_sort identification of amyloidogenic regions in pseudomonas aeruginosa ribosomal s1 protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305250/
https://www.ncbi.nlm.nih.gov/pubmed/34298910
http://dx.doi.org/10.3390/ijms22147291
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