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Binding interactions with sevelamer and polystyrene sulfonate in vitro

This study explored the binding of 28 drugs, which were selected based on frequency of concomitant use and chemical properties, to sevelamer and polystyrene sulfonate in vitro. The relative binding was determined by dissolving the investigated drugs alone (=control), together with 800 mg of sevelame...

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Autores principales: van Berlo‐van de Laar, Inge R.F., Prins‐Can, Ilona, de Lange, Aliesa A., Taxis, Katja, Jansman, Frank G.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305432/
https://www.ncbi.nlm.nih.gov/pubmed/34302439
http://dx.doi.org/10.1002/prp2.834
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author van Berlo‐van de Laar, Inge R.F.
Prins‐Can, Ilona
de Lange, Aliesa A.
Taxis, Katja
Jansman, Frank G.A.
author_facet van Berlo‐van de Laar, Inge R.F.
Prins‐Can, Ilona
de Lange, Aliesa A.
Taxis, Katja
Jansman, Frank G.A.
author_sort van Berlo‐van de Laar, Inge R.F.
collection PubMed
description This study explored the binding of 28 drugs, which were selected based on frequency of concomitant use and chemical properties, to sevelamer and polystyrene sulfonate in vitro. The relative binding was determined by dissolving the investigated drugs alone (=control), together with 800 mg of sevelamer and 15 g of polystyrene sulfonate at different pH levels (1.5, 5.5, and 7.4), respectively. After incubation at 37℃ and shaking for 60 min, the solutions were diluted and centrifuged, and the drug concentrations were quantified with validated analytical assays. The binding assays were performed in threefold. The mean relative binding (MRB) at each pH level was calculated, with a MRB >20% for at least one pH level to be considered as relevant binding. Fourteen and 23 potentially new binding interactions were identified with sevelamer and polystyrene sulfonate, respectively. These potentially new binding interactions have to be studied in vivo to assess their clinical relevance.
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spelling pubmed-83054322021-07-28 Binding interactions with sevelamer and polystyrene sulfonate in vitro van Berlo‐van de Laar, Inge R.F. Prins‐Can, Ilona de Lange, Aliesa A. Taxis, Katja Jansman, Frank G.A. Pharmacol Res Perspect Original Articles This study explored the binding of 28 drugs, which were selected based on frequency of concomitant use and chemical properties, to sevelamer and polystyrene sulfonate in vitro. The relative binding was determined by dissolving the investigated drugs alone (=control), together with 800 mg of sevelamer and 15 g of polystyrene sulfonate at different pH levels (1.5, 5.5, and 7.4), respectively. After incubation at 37℃ and shaking for 60 min, the solutions were diluted and centrifuged, and the drug concentrations were quantified with validated analytical assays. The binding assays were performed in threefold. The mean relative binding (MRB) at each pH level was calculated, with a MRB >20% for at least one pH level to be considered as relevant binding. Fourteen and 23 potentially new binding interactions were identified with sevelamer and polystyrene sulfonate, respectively. These potentially new binding interactions have to be studied in vivo to assess their clinical relevance. John Wiley and Sons Inc. 2021-07-24 /pmc/articles/PMC8305432/ /pubmed/34302439 http://dx.doi.org/10.1002/prp2.834 Text en © 2021 The Authors. Pharmacology Research & Perspectives published by British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
van Berlo‐van de Laar, Inge R.F.
Prins‐Can, Ilona
de Lange, Aliesa A.
Taxis, Katja
Jansman, Frank G.A.
Binding interactions with sevelamer and polystyrene sulfonate in vitro
title Binding interactions with sevelamer and polystyrene sulfonate in vitro
title_full Binding interactions with sevelamer and polystyrene sulfonate in vitro
title_fullStr Binding interactions with sevelamer and polystyrene sulfonate in vitro
title_full_unstemmed Binding interactions with sevelamer and polystyrene sulfonate in vitro
title_short Binding interactions with sevelamer and polystyrene sulfonate in vitro
title_sort binding interactions with sevelamer and polystyrene sulfonate in vitro
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305432/
https://www.ncbi.nlm.nih.gov/pubmed/34302439
http://dx.doi.org/10.1002/prp2.834
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