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Binding interactions with sevelamer and polystyrene sulfonate in vitro
This study explored the binding of 28 drugs, which were selected based on frequency of concomitant use and chemical properties, to sevelamer and polystyrene sulfonate in vitro. The relative binding was determined by dissolving the investigated drugs alone (=control), together with 800 mg of sevelame...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305432/ https://www.ncbi.nlm.nih.gov/pubmed/34302439 http://dx.doi.org/10.1002/prp2.834 |
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author | van Berlo‐van de Laar, Inge R.F. Prins‐Can, Ilona de Lange, Aliesa A. Taxis, Katja Jansman, Frank G.A. |
author_facet | van Berlo‐van de Laar, Inge R.F. Prins‐Can, Ilona de Lange, Aliesa A. Taxis, Katja Jansman, Frank G.A. |
author_sort | van Berlo‐van de Laar, Inge R.F. |
collection | PubMed |
description | This study explored the binding of 28 drugs, which were selected based on frequency of concomitant use and chemical properties, to sevelamer and polystyrene sulfonate in vitro. The relative binding was determined by dissolving the investigated drugs alone (=control), together with 800 mg of sevelamer and 15 g of polystyrene sulfonate at different pH levels (1.5, 5.5, and 7.4), respectively. After incubation at 37℃ and shaking for 60 min, the solutions were diluted and centrifuged, and the drug concentrations were quantified with validated analytical assays. The binding assays were performed in threefold. The mean relative binding (MRB) at each pH level was calculated, with a MRB >20% for at least one pH level to be considered as relevant binding. Fourteen and 23 potentially new binding interactions were identified with sevelamer and polystyrene sulfonate, respectively. These potentially new binding interactions have to be studied in vivo to assess their clinical relevance. |
format | Online Article Text |
id | pubmed-8305432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83054322021-07-28 Binding interactions with sevelamer and polystyrene sulfonate in vitro van Berlo‐van de Laar, Inge R.F. Prins‐Can, Ilona de Lange, Aliesa A. Taxis, Katja Jansman, Frank G.A. Pharmacol Res Perspect Original Articles This study explored the binding of 28 drugs, which were selected based on frequency of concomitant use and chemical properties, to sevelamer and polystyrene sulfonate in vitro. The relative binding was determined by dissolving the investigated drugs alone (=control), together with 800 mg of sevelamer and 15 g of polystyrene sulfonate at different pH levels (1.5, 5.5, and 7.4), respectively. After incubation at 37℃ and shaking for 60 min, the solutions were diluted and centrifuged, and the drug concentrations were quantified with validated analytical assays. The binding assays were performed in threefold. The mean relative binding (MRB) at each pH level was calculated, with a MRB >20% for at least one pH level to be considered as relevant binding. Fourteen and 23 potentially new binding interactions were identified with sevelamer and polystyrene sulfonate, respectively. These potentially new binding interactions have to be studied in vivo to assess their clinical relevance. John Wiley and Sons Inc. 2021-07-24 /pmc/articles/PMC8305432/ /pubmed/34302439 http://dx.doi.org/10.1002/prp2.834 Text en © 2021 The Authors. Pharmacology Research & Perspectives published by British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles van Berlo‐van de Laar, Inge R.F. Prins‐Can, Ilona de Lange, Aliesa A. Taxis, Katja Jansman, Frank G.A. Binding interactions with sevelamer and polystyrene sulfonate in vitro |
title | Binding interactions with sevelamer and polystyrene sulfonate in vitro
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title_full | Binding interactions with sevelamer and polystyrene sulfonate in vitro
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title_fullStr | Binding interactions with sevelamer and polystyrene sulfonate in vitro
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title_full_unstemmed | Binding interactions with sevelamer and polystyrene sulfonate in vitro
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title_short | Binding interactions with sevelamer and polystyrene sulfonate in vitro
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title_sort | binding interactions with sevelamer and polystyrene sulfonate in vitro |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305432/ https://www.ncbi.nlm.nih.gov/pubmed/34302439 http://dx.doi.org/10.1002/prp2.834 |
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