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High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature

Primary ciliary dyskinesia (PCD) is a rare disease resulting from a defect in ciliary function that generates, among other issues, chronic upper and lower respiratory tract infections. European guidelines recommend studying ciliary function (pattern (CBP) and frequency (CBF)), together with characte...

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Autores principales: Reula, Ana, Pitarch-Fabregat, Javier, Milara, Javier, Cortijo, Julio, Mata-Roig, Manuel, Milian, Lara, Armengot, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305583/
https://www.ncbi.nlm.nih.gov/pubmed/34359383
http://dx.doi.org/10.3390/diagnostics11071301
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author Reula, Ana
Pitarch-Fabregat, Javier
Milara, Javier
Cortijo, Julio
Mata-Roig, Manuel
Milian, Lara
Armengot, Miguel
author_facet Reula, Ana
Pitarch-Fabregat, Javier
Milara, Javier
Cortijo, Julio
Mata-Roig, Manuel
Milian, Lara
Armengot, Miguel
author_sort Reula, Ana
collection PubMed
description Primary ciliary dyskinesia (PCD) is a rare disease resulting from a defect in ciliary function that generates, among other issues, chronic upper and lower respiratory tract infections. European guidelines recommend studying ciliary function (pattern (CBP) and frequency (CBF)), together with characteristic clinical symptoms, as one of the definitive tests. However, there is no “gold standard”. The present study aims to use high-speed video microscopy to describe how CBF and CBP alter over time and at different temperatures to reduce the error rate in the diagnosis of PCD. Samples of nasal epithelium from 27 healthy volunteers were studied to assess CBF and CBP at 0, 3, 24, 48, and 72 h, at room temperature and 4 °C. It was observed that CBF increased while CBP became dyskinetic, both at room temperature and at 4 °C, as time passed, especially after 3 h. In order to preserve all ciliary function parameters and to perform a reliable analysis to improve the diagnostic process of PCD, analysis should be performed within the first 3 h of sample collection, preferably in reference centers.
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spelling pubmed-83055832021-07-25 High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature Reula, Ana Pitarch-Fabregat, Javier Milara, Javier Cortijo, Julio Mata-Roig, Manuel Milian, Lara Armengot, Miguel Diagnostics (Basel) Article Primary ciliary dyskinesia (PCD) is a rare disease resulting from a defect in ciliary function that generates, among other issues, chronic upper and lower respiratory tract infections. European guidelines recommend studying ciliary function (pattern (CBP) and frequency (CBF)), together with characteristic clinical symptoms, as one of the definitive tests. However, there is no “gold standard”. The present study aims to use high-speed video microscopy to describe how CBF and CBP alter over time and at different temperatures to reduce the error rate in the diagnosis of PCD. Samples of nasal epithelium from 27 healthy volunteers were studied to assess CBF and CBP at 0, 3, 24, 48, and 72 h, at room temperature and 4 °C. It was observed that CBF increased while CBP became dyskinetic, both at room temperature and at 4 °C, as time passed, especially after 3 h. In order to preserve all ciliary function parameters and to perform a reliable analysis to improve the diagnostic process of PCD, analysis should be performed within the first 3 h of sample collection, preferably in reference centers. MDPI 2021-07-20 /pmc/articles/PMC8305583/ /pubmed/34359383 http://dx.doi.org/10.3390/diagnostics11071301 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reula, Ana
Pitarch-Fabregat, Javier
Milara, Javier
Cortijo, Julio
Mata-Roig, Manuel
Milian, Lara
Armengot, Miguel
High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature
title High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature
title_full High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature
title_fullStr High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature
title_full_unstemmed High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature
title_short High-Speed Video Microscopy for Primary Ciliary Dyskinesia Diagnosis: A Study of Ciliary Motility Variations with Time and Temperature
title_sort high-speed video microscopy for primary ciliary dyskinesia diagnosis: a study of ciliary motility variations with time and temperature
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305583/
https://www.ncbi.nlm.nih.gov/pubmed/34359383
http://dx.doi.org/10.3390/diagnostics11071301
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