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Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method

This study was carried out to develop a high-performance liquid chromatography method for short-time analysis of the main cannabinoids in the inflorescence of hemp (Cannabis sativa L.). We also performed decarboxylation of the raw material using our advanced analysis technique. In this study, the UV...

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Autores principales: Ryu, Byeong Ryeol, Islam, Md. Jahirul, Azad, Md. Obyedul Kalam, Go, Eun-Ji, Rahman, Md. Hafizur, Rana, Md. Soyel, Lim, Young-Seok, Lim, Jung-Dae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306309/
https://www.ncbi.nlm.nih.gov/pubmed/34299386
http://dx.doi.org/10.3390/molecules26144113
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author Ryu, Byeong Ryeol
Islam, Md. Jahirul
Azad, Md. Obyedul Kalam
Go, Eun-Ji
Rahman, Md. Hafizur
Rana, Md. Soyel
Lim, Young-Seok
Lim, Jung-Dae
author_facet Ryu, Byeong Ryeol
Islam, Md. Jahirul
Azad, Md. Obyedul Kalam
Go, Eun-Ji
Rahman, Md. Hafizur
Rana, Md. Soyel
Lim, Young-Seok
Lim, Jung-Dae
author_sort Ryu, Byeong Ryeol
collection PubMed
description This study was carried out to develop a high-performance liquid chromatography method for short-time analysis of the main cannabinoids in the inflorescence of hemp (Cannabis sativa L.). We also performed decarboxylation of the raw material using our advanced analysis technique. In this study, the UV spectrum was considered to analyze each of the four common cannabinoids, solvents, and samples, where the uniform elution of acidic cannabinoids without peak tailing and acids was tested. Optimal results were obtained when readings were taken at a wavelength of 220 nm using water and methanol containing trifluoroacetic acid as mobile phases in a solvent gradient system. The established conditions were further validated by system suitability, linearity, precision, detection limit, and quantitation limit tests. The decarboxylation index (DT(50)) confirmed that Δ9-THCA decarboxylated faster than CBDA, and both maintained a linear relationship with time and temperature. In addition, the loss of cannabidiol was better prevented during the decarboxylation process in the natural state than in the extracted state.
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spelling pubmed-83063092021-07-25 Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method Ryu, Byeong Ryeol Islam, Md. Jahirul Azad, Md. Obyedul Kalam Go, Eun-Ji Rahman, Md. Hafizur Rana, Md. Soyel Lim, Young-Seok Lim, Jung-Dae Molecules Article This study was carried out to develop a high-performance liquid chromatography method for short-time analysis of the main cannabinoids in the inflorescence of hemp (Cannabis sativa L.). We also performed decarboxylation of the raw material using our advanced analysis technique. In this study, the UV spectrum was considered to analyze each of the four common cannabinoids, solvents, and samples, where the uniform elution of acidic cannabinoids without peak tailing and acids was tested. Optimal results were obtained when readings were taken at a wavelength of 220 nm using water and methanol containing trifluoroacetic acid as mobile phases in a solvent gradient system. The established conditions were further validated by system suitability, linearity, precision, detection limit, and quantitation limit tests. The decarboxylation index (DT(50)) confirmed that Δ9-THCA decarboxylated faster than CBDA, and both maintained a linear relationship with time and temperature. In addition, the loss of cannabidiol was better prevented during the decarboxylation process in the natural state than in the extracted state. MDPI 2021-07-06 /pmc/articles/PMC8306309/ /pubmed/34299386 http://dx.doi.org/10.3390/molecules26144113 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ryu, Byeong Ryeol
Islam, Md. Jahirul
Azad, Md. Obyedul Kalam
Go, Eun-Ji
Rahman, Md. Hafizur
Rana, Md. Soyel
Lim, Young-Seok
Lim, Jung-Dae
Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method
title Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method
title_full Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method
title_fullStr Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method
title_full_unstemmed Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method
title_short Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method
title_sort conversion characteristics of some major cannabinoids from hemp (cannabis sativa l.) raw materials by new rapid simultaneous analysis method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306309/
https://www.ncbi.nlm.nih.gov/pubmed/34299386
http://dx.doi.org/10.3390/molecules26144113
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