Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study

OBJECTIVE: Extracellular histones have been identified as one molecular factor that can cause and sustain alveolar damage and were linked to high mortality rates in critically ill patients. In this pilot study, we wanted to validate the proinflammatory in vivo effects of local histone application in...

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Autores principales: Ruemmler, Robert, Ziebart, Alexander, Britten, Elisabeth, Gosling, Moritz, Rissel, Rene, Hartmann, Erik K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306385/
https://www.ncbi.nlm.nih.gov/pubmed/34301315
http://dx.doi.org/10.1186/s13104-021-05704-7
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author Ruemmler, Robert
Ziebart, Alexander
Britten, Elisabeth
Gosling, Moritz
Rissel, Rene
Hartmann, Erik K.
author_facet Ruemmler, Robert
Ziebart, Alexander
Britten, Elisabeth
Gosling, Moritz
Rissel, Rene
Hartmann, Erik K.
author_sort Ruemmler, Robert
collection PubMed
description OBJECTIVE: Extracellular histones have been identified as one molecular factor that can cause and sustain alveolar damage and were linked to high mortality rates in critically ill patients. In this pilot study, we wanted to validate the proinflammatory in vivo effects of local histone application in a prospective translational porcine model. This was combined with the evaluation of an experimental acute lung injury model using intrabronchial lipopolysaccharides, which has been published previously. RESULTS: The targeted application of histones was successful in all animals. Animals showed decreased oxygenation after instillation, but no differences could be detected between the sham and histone treatments. The histologic analyses and inflammatory responses indicated that there were no differences in tissue damage between the groups. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-021-05704-7.
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spelling pubmed-83063852021-07-28 Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study Ruemmler, Robert Ziebart, Alexander Britten, Elisabeth Gosling, Moritz Rissel, Rene Hartmann, Erik K. BMC Res Notes Research Note OBJECTIVE: Extracellular histones have been identified as one molecular factor that can cause and sustain alveolar damage and were linked to high mortality rates in critically ill patients. In this pilot study, we wanted to validate the proinflammatory in vivo effects of local histone application in a prospective translational porcine model. This was combined with the evaluation of an experimental acute lung injury model using intrabronchial lipopolysaccharides, which has been published previously. RESULTS: The targeted application of histones was successful in all animals. Animals showed decreased oxygenation after instillation, but no differences could be detected between the sham and histone treatments. The histologic analyses and inflammatory responses indicated that there were no differences in tissue damage between the groups. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-021-05704-7. BioMed Central 2021-07-23 /pmc/articles/PMC8306385/ /pubmed/34301315 http://dx.doi.org/10.1186/s13104-021-05704-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Ruemmler, Robert
Ziebart, Alexander
Britten, Elisabeth
Gosling, Moritz
Rissel, Rene
Hartmann, Erik K.
Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study
title Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study
title_full Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study
title_fullStr Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study
title_full_unstemmed Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study
title_short Intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study
title_sort intrabronchial application of extracellular histones shows no proinflammatory effects in swine in a translational pilot study
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306385/
https://www.ncbi.nlm.nih.gov/pubmed/34301315
http://dx.doi.org/10.1186/s13104-021-05704-7
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