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Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles

This study involved the preparation and characterization of structures with a honeycomb-like pattern (HCP) formed using the phase separation method using a solution mixture of chloroform and methanol together with cellulose acetate. Fluorinated ethylene propylene modified by plasma treatment was use...

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Autores principales: Hurtuková, Klaudia, Fajstavrová, Klára, Rimpelová, Silvie, Vokatá, Barbora, Fajstavr, Dominik, Kasálková, Nikola Slepičková, Siegel, Jakub, Švorčík, Václav, Slepička, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306805/
https://www.ncbi.nlm.nih.gov/pubmed/34300969
http://dx.doi.org/10.3390/ma14144051
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author Hurtuková, Klaudia
Fajstavrová, Klára
Rimpelová, Silvie
Vokatá, Barbora
Fajstavr, Dominik
Kasálková, Nikola Slepičková
Siegel, Jakub
Švorčík, Václav
Slepička, Petr
author_facet Hurtuková, Klaudia
Fajstavrová, Klára
Rimpelová, Silvie
Vokatá, Barbora
Fajstavr, Dominik
Kasálková, Nikola Slepičková
Siegel, Jakub
Švorčík, Václav
Slepička, Petr
author_sort Hurtuková, Klaudia
collection PubMed
description This study involved the preparation and characterization of structures with a honeycomb-like pattern (HCP) formed using the phase separation method using a solution mixture of chloroform and methanol together with cellulose acetate. Fluorinated ethylene propylene modified by plasma treatment was used as a suitable substrate for the formation of the HCP structures. Further, we modified the HCP structures using silver sputtering (discontinuous Ag nanoparticles) or by adding Ag nanoparticles in PEG into the cellulose acetate solution. The material morphology was then determined using atomic force microscopy (AFM) and scanning electron microscopy (SEM), while the material surface chemistry was studied using energy dispersive spectroscopy (EDS) and wettability was analyzed with goniometry. The AFM and SEM results revealed that the surface morphology of pristine HCP with hexagonal pores changed after additional sample modification with Ag, both via the addition of nanoparticles and sputtering, accompanied with an increase in the roughness of the PEG-doped samples, which was caused by the high molecular weight of PEG and its gel-like structure. The highest amount (approx. 25 at %) of fluorine was detected using the EDS method on the sample with an HCP-like structure, while the lowest amount (0.08%) was measured on the PEG + Ag sample, which revealed the covering of the substrate with biopolymer (the greater fluorine extent means more of the fluorinated substrate is exposed). As expected, the thickness of the Ag layer on the HCP surface depended on the length of sputtering (either 150 s or 500 s). The sputtering times for Ag (150 s and 500 s) corresponded to layers with heights of about 8 nm (3.9 at % of Ag) and 22 nm (10.8 at % of Ag), respectively. In addition, we evaluated the antibacterial potential of the prepared substrate using two bacterial strains, one Gram-positive of S. epidermidis and one Gram-negative of E. coli. The most effective method for the construction of antibacterial surfaces was determined to be sputtering (150 s) of a silver nanolayer onto a HCP-like cellulose structure, which proved to have excellent antibacterial properties against both G+ and G− bacterial strains.
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spelling pubmed-83068052021-07-25 Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles Hurtuková, Klaudia Fajstavrová, Klára Rimpelová, Silvie Vokatá, Barbora Fajstavr, Dominik Kasálková, Nikola Slepičková Siegel, Jakub Švorčík, Václav Slepička, Petr Materials (Basel) Article This study involved the preparation and characterization of structures with a honeycomb-like pattern (HCP) formed using the phase separation method using a solution mixture of chloroform and methanol together with cellulose acetate. Fluorinated ethylene propylene modified by plasma treatment was used as a suitable substrate for the formation of the HCP structures. Further, we modified the HCP structures using silver sputtering (discontinuous Ag nanoparticles) or by adding Ag nanoparticles in PEG into the cellulose acetate solution. The material morphology was then determined using atomic force microscopy (AFM) and scanning electron microscopy (SEM), while the material surface chemistry was studied using energy dispersive spectroscopy (EDS) and wettability was analyzed with goniometry. The AFM and SEM results revealed that the surface morphology of pristine HCP with hexagonal pores changed after additional sample modification with Ag, both via the addition of nanoparticles and sputtering, accompanied with an increase in the roughness of the PEG-doped samples, which was caused by the high molecular weight of PEG and its gel-like structure. The highest amount (approx. 25 at %) of fluorine was detected using the EDS method on the sample with an HCP-like structure, while the lowest amount (0.08%) was measured on the PEG + Ag sample, which revealed the covering of the substrate with biopolymer (the greater fluorine extent means more of the fluorinated substrate is exposed). As expected, the thickness of the Ag layer on the HCP surface depended on the length of sputtering (either 150 s or 500 s). The sputtering times for Ag (150 s and 500 s) corresponded to layers with heights of about 8 nm (3.9 at % of Ag) and 22 nm (10.8 at % of Ag), respectively. In addition, we evaluated the antibacterial potential of the prepared substrate using two bacterial strains, one Gram-positive of S. epidermidis and one Gram-negative of E. coli. The most effective method for the construction of antibacterial surfaces was determined to be sputtering (150 s) of a silver nanolayer onto a HCP-like cellulose structure, which proved to have excellent antibacterial properties against both G+ and G− bacterial strains. MDPI 2021-07-20 /pmc/articles/PMC8306805/ /pubmed/34300969 http://dx.doi.org/10.3390/ma14144051 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hurtuková, Klaudia
Fajstavrová, Klára
Rimpelová, Silvie
Vokatá, Barbora
Fajstavr, Dominik
Kasálková, Nikola Slepičková
Siegel, Jakub
Švorčík, Václav
Slepička, Petr
Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles
title Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles
title_full Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles
title_fullStr Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles
title_full_unstemmed Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles
title_short Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles
title_sort antibacterial properties of a honeycomb-like pattern with cellulose acetate and silver nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306805/
https://www.ncbi.nlm.nih.gov/pubmed/34300969
http://dx.doi.org/10.3390/ma14144051
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