Cargando…
Human Species D Adenoviruses Isolated from Diarrheal Feces Show Low Infection Rates in Primary Nasal Epithelial Cells
The importance of adenovirus (Ad) research is significantly increasing with respect to virotherapy for vaccine development, tumor, and gene therapy. Due to the different species and subtypes of this virus, the characterization of the biological significance of especially rare Ad is necessary. Previo...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307086/ https://www.ncbi.nlm.nih.gov/pubmed/34208817 http://dx.doi.org/10.3390/children8070563 |
_version_ | 1783727966411292672 |
---|---|
author | Aydin, Malik Schellhorn, Sebastian Wirth, Stefan Zhang, Wenli Ehrhardt, Anja |
author_facet | Aydin, Malik Schellhorn, Sebastian Wirth, Stefan Zhang, Wenli Ehrhardt, Anja |
author_sort | Aydin, Malik |
collection | PubMed |
description | The importance of adenovirus (Ad) research is significantly increasing with respect to virotherapy for vaccine development, tumor, and gene therapy. Due to the different species and subtypes of this virus, the characterization of the biological significance of especially rare Ad is necessary. Previously, rare Ad types 70, 73, and 74 were originally isolated from fecal samples of immunocompromised patients and they represent recombinants of other Ad types. Here we investigated transduction experiments of these reporter gene tagged Ad types in primary cells exemplified by subject-derived primary nasal epithelial cells (NAEPCs). To analyze the transduction rates, we performed flow cytometry, quantitative polymerase chain reaction (PCR), and cytokine analyses 25 h post-infection. We found that, in contrast to Ad type 5 (as a positive control), the transduction rates of NAEPCs with Ad types 70, 73, and 74 were interestingly low. The major Ad receptor (coxsackievirus-adenovirus receptor and CD46) expression levels showed no significant change after infection with Ad types 70, 73 and 74. Moreover, Interleukin 6 (IL-6) was not released after in vitro Ad transduction. Due to the high risk of developing life-threatening complications in immunocompromised patients by these human species D Ads, even more attention needs to be investigated into the development of diagnostic and therapeutic concepts to prevent and treat those opportunistic infections in susceptible patients. |
format | Online Article Text |
id | pubmed-8307086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83070862021-07-25 Human Species D Adenoviruses Isolated from Diarrheal Feces Show Low Infection Rates in Primary Nasal Epithelial Cells Aydin, Malik Schellhorn, Sebastian Wirth, Stefan Zhang, Wenli Ehrhardt, Anja Children (Basel) Brief Report The importance of adenovirus (Ad) research is significantly increasing with respect to virotherapy for vaccine development, tumor, and gene therapy. Due to the different species and subtypes of this virus, the characterization of the biological significance of especially rare Ad is necessary. Previously, rare Ad types 70, 73, and 74 were originally isolated from fecal samples of immunocompromised patients and they represent recombinants of other Ad types. Here we investigated transduction experiments of these reporter gene tagged Ad types in primary cells exemplified by subject-derived primary nasal epithelial cells (NAEPCs). To analyze the transduction rates, we performed flow cytometry, quantitative polymerase chain reaction (PCR), and cytokine analyses 25 h post-infection. We found that, in contrast to Ad type 5 (as a positive control), the transduction rates of NAEPCs with Ad types 70, 73, and 74 were interestingly low. The major Ad receptor (coxsackievirus-adenovirus receptor and CD46) expression levels showed no significant change after infection with Ad types 70, 73 and 74. Moreover, Interleukin 6 (IL-6) was not released after in vitro Ad transduction. Due to the high risk of developing life-threatening complications in immunocompromised patients by these human species D Ads, even more attention needs to be investigated into the development of diagnostic and therapeutic concepts to prevent and treat those opportunistic infections in susceptible patients. MDPI 2021-06-30 /pmc/articles/PMC8307086/ /pubmed/34208817 http://dx.doi.org/10.3390/children8070563 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Aydin, Malik Schellhorn, Sebastian Wirth, Stefan Zhang, Wenli Ehrhardt, Anja Human Species D Adenoviruses Isolated from Diarrheal Feces Show Low Infection Rates in Primary Nasal Epithelial Cells |
title | Human Species D Adenoviruses Isolated from Diarrheal Feces Show Low Infection Rates in Primary Nasal Epithelial Cells |
title_full | Human Species D Adenoviruses Isolated from Diarrheal Feces Show Low Infection Rates in Primary Nasal Epithelial Cells |
title_fullStr | Human Species D Adenoviruses Isolated from Diarrheal Feces Show Low Infection Rates in Primary Nasal Epithelial Cells |
title_full_unstemmed | Human Species D Adenoviruses Isolated from Diarrheal Feces Show Low Infection Rates in Primary Nasal Epithelial Cells |
title_short | Human Species D Adenoviruses Isolated from Diarrheal Feces Show Low Infection Rates in Primary Nasal Epithelial Cells |
title_sort | human species d adenoviruses isolated from diarrheal feces show low infection rates in primary nasal epithelial cells |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307086/ https://www.ncbi.nlm.nih.gov/pubmed/34208817 http://dx.doi.org/10.3390/children8070563 |
work_keys_str_mv | AT aydinmalik humanspeciesdadenovirusesisolatedfromdiarrhealfecesshowlowinfectionratesinprimarynasalepithelialcells AT schellhornsebastian humanspeciesdadenovirusesisolatedfromdiarrhealfecesshowlowinfectionratesinprimarynasalepithelialcells AT wirthstefan humanspeciesdadenovirusesisolatedfromdiarrhealfecesshowlowinfectionratesinprimarynasalepithelialcells AT zhangwenli humanspeciesdadenovirusesisolatedfromdiarrhealfecesshowlowinfectionratesinprimarynasalepithelialcells AT ehrhardtanja humanspeciesdadenovirusesisolatedfromdiarrhealfecesshowlowinfectionratesinprimarynasalepithelialcells |