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Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes

Lymph node mapping is important in cancer immunotherapy because the morphology of lymph nodes is one of the crucial evaluation criteria of immune responses. We developed new theragnostic glycol-chitosan-coated gold nanoparticles (GC-AuNPs), which highlighted lymph nodes in ultrasound-guided photoaco...

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Autores principales: Sun, In-Cheol, Jo, SeongHoon, Dumani, Diego, Yun, Wan Su, Yoon, Hong Yeol, Lim, Dong-Kwon, Ahn, Cheol-Hee, Emelianov, Stanislav, Kim, Kwangmeyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307152/
https://www.ncbi.nlm.nih.gov/pubmed/34203541
http://dx.doi.org/10.3390/nano11071700
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author Sun, In-Cheol
Jo, SeongHoon
Dumani, Diego
Yun, Wan Su
Yoon, Hong Yeol
Lim, Dong-Kwon
Ahn, Cheol-Hee
Emelianov, Stanislav
Kim, Kwangmeyung
author_facet Sun, In-Cheol
Jo, SeongHoon
Dumani, Diego
Yun, Wan Su
Yoon, Hong Yeol
Lim, Dong-Kwon
Ahn, Cheol-Hee
Emelianov, Stanislav
Kim, Kwangmeyung
author_sort Sun, In-Cheol
collection PubMed
description Lymph node mapping is important in cancer immunotherapy because the morphology of lymph nodes is one of the crucial evaluation criteria of immune responses. We developed new theragnostic glycol-chitosan-coated gold nanoparticles (GC-AuNPs), which highlighted lymph nodes in ultrasound-guided photoacoustic (US/PA) imaging. Moreover, the ovalbumin epitope was conjugated GC-AuNPs (OVA-GC-AuNPs) for delivering tumor antigen to lymph node resident macrophage. In vitro studies proved the vigorous endocytosis activity of J774A.1 macrophage and consequent strong photoacoustic signals from them. The macrophages also presented a tumor antigen when OVA-GC-AuNPs were used for cellular uptake. After the lingual injection of GC-AuNPs into healthy mice, cervical lymph nodes were visible in a US/PA imaging system with high contrast. Three-dimensional analysis of lymph nodes revealed that the accumulation of GC-AuNPs in the lymph node increased as the post-injection time passed. Histological analysis showed GC-AuNPs or OVA-GC-AuNPs located in subcapsular and medullar sinuses where macrophages are abundant. Our new theragnostic GC-AuNPs present a superior performance in US/PA imaging of lymph nodes without targeting moieties or complex surface modification. Simultaneously, GC-AuNPs were able to deliver tumor antigens to cause macrophages to present the OVA epitope at targeted lymph nodes, which would be valuable for cancer immunotherapy.
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spelling pubmed-83071522021-07-25 Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes Sun, In-Cheol Jo, SeongHoon Dumani, Diego Yun, Wan Su Yoon, Hong Yeol Lim, Dong-Kwon Ahn, Cheol-Hee Emelianov, Stanislav Kim, Kwangmeyung Nanomaterials (Basel) Article Lymph node mapping is important in cancer immunotherapy because the morphology of lymph nodes is one of the crucial evaluation criteria of immune responses. We developed new theragnostic glycol-chitosan-coated gold nanoparticles (GC-AuNPs), which highlighted lymph nodes in ultrasound-guided photoacoustic (US/PA) imaging. Moreover, the ovalbumin epitope was conjugated GC-AuNPs (OVA-GC-AuNPs) for delivering tumor antigen to lymph node resident macrophage. In vitro studies proved the vigorous endocytosis activity of J774A.1 macrophage and consequent strong photoacoustic signals from them. The macrophages also presented a tumor antigen when OVA-GC-AuNPs were used for cellular uptake. After the lingual injection of GC-AuNPs into healthy mice, cervical lymph nodes were visible in a US/PA imaging system with high contrast. Three-dimensional analysis of lymph nodes revealed that the accumulation of GC-AuNPs in the lymph node increased as the post-injection time passed. Histological analysis showed GC-AuNPs or OVA-GC-AuNPs located in subcapsular and medullar sinuses where macrophages are abundant. Our new theragnostic GC-AuNPs present a superior performance in US/PA imaging of lymph nodes without targeting moieties or complex surface modification. Simultaneously, GC-AuNPs were able to deliver tumor antigens to cause macrophages to present the OVA epitope at targeted lymph nodes, which would be valuable for cancer immunotherapy. MDPI 2021-06-28 /pmc/articles/PMC8307152/ /pubmed/34203541 http://dx.doi.org/10.3390/nano11071700 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, In-Cheol
Jo, SeongHoon
Dumani, Diego
Yun, Wan Su
Yoon, Hong Yeol
Lim, Dong-Kwon
Ahn, Cheol-Hee
Emelianov, Stanislav
Kim, Kwangmeyung
Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes
title Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes
title_full Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes
title_fullStr Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes
title_full_unstemmed Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes
title_short Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes
title_sort theragnostic glycol chitosan-conjugated gold nanoparticles for photoacoustic imaging of regional lymph nodes and delivering tumor antigen to lymph nodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307152/
https://www.ncbi.nlm.nih.gov/pubmed/34203541
http://dx.doi.org/10.3390/nano11071700
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